The effect of aqueous and ethanolic extracts of Artemisia herba alba on human laryngeal carcinoma and murine mammary adenocarcinoma cell lines

The present study was carried out to evaluate the cytological effects of aqueous (AE) and ethanolic (EE) extracts of Artemisia herba alba on human laryngeal carcinoma (Hep-2) cell line and murine mammary adenocarcinoma (AMN-3) cell line in vitro. The cytological study performed simultaneously with cell growth assay. The results of study revealed concentration-dependent cytological changes like patchy growth inhibition, loss of confluent feature and cellular degeneration after exposure to the lowest concentrations (156.25 and 312.5 μg/ml). The early findings of cytolysis were seen after exposure to 625 μg/ml. While the highest concentrations (1250, 2500 and 5000 μg/ml) caused severe growth inhibition with marked cytolytic features including loss of cellular outlines, large numbers of dead cells and high content of cellular debris. In conclusion, the results of this study revealed the high cytological effect of Artemisia herba alba extracts on Hep-2 and AMN-3 cell lines in vitro

SNUFFLES DISEASE IN RABBITS 1-CLINICAL AND BACTERIOLOGICAL STUDY

ABSTRACT Snuffles disease is a major problem in rabbits caused by pasteurella multocida, for the importance of this disease, this study aimed to identify clinical and bacteriological findings associated with experimental P. multocida infection in rabbits. Two groups of rabbits: 1 st group (7) control group injected 1/p with pbs. 2 nd group infected group (27) injected I/p with 10 2 CFU/ml of P. multocida CLD50 was 10 7 CFU/ml. Different clinical signs were observed in rabbits at 1 st -20 th day post infection, the clinical signs included excitation, then dullness, anorexia, fever, congestion of conjunctiva and loss of weight and mucopurulent nasal discharge later on bacterial dissemination, P. multocida were isolated from the different organs of the infected rabbits at 4 th , 8 th , 12 th , 16 th and 20 th days post infection. The levels of the bacterial isolates were very heavy, heavy and moderate at the beginning and mild and very mild isolates at the end of infection