Citokemija stanica koštane srži u ovaca

The present study was conducted on bone marrow samples obtained from 15 clinically normal Libyan Barbary sheep. Haemopoietic cells, including those of myelocytic series, erythrocytic series, megakaryocytic series, lymphocytes, plasma cells, monocytes and mitotic cells were identified on the basis of their morphological characteristics in May-Grünwald-Giemsa stained bone marrow smears. Cytochemical reactions of bone marrow cells revealed that all granulocytes, except myeloblasts and basophils, were peroxidase positive. Monocytes were peroxidase negative, but very few of them showed weak activity. Cells of the erythrocytic, megakaryocytic and lymphocytic series were peroxidase negative. Granulocytic series cells were Sudan black B positive, except myeloblasts. Some monocytes were positive with a few scattered black granules, while others were negative. Cells of erythrocytic, megakaryocytic and lymphocytic series were Sudan black B negative. Granulocytes at all stages of development reacted positively to periodic acid-Shiff, except myeloblasts. Monocytes were either completely negative or positive. Some lymphocytes showed a few fine or even coarse periodic acid-Shiff positive granules in the cytoplasm. Megakaryocytes were periodic acid-Shiff positive, while all cells of the erythrocytic series were periodic acid-Shiff negative. There was agreement in the mean values of the percentages of the three cytochemical stains positive cells and May-Grünwald-Giemsa stained bone marrow granulocytes.Istraživanje je provedeno na uzorcima koštane srži 15 klinički zdravih libijskih berberskih ovaca. Hemopoetske stanice, uključujući one mijeloične, eritrocitne i megakariocitne linije, limfociti, plazma stanice, monociti i mitotičke stanice bile su identificirane na osnovi njihovih morfoloških značajki u razmascima koštane srži obojenima po May-Grünwald-Giemsi. Citokemijske reakcije stanica koštane srži pokazale su da su svi granulociti, osim mijeloblasta i bazofila, bili pozitivni na peroksidazu. Monociti su bili negativni na peroksidazu, iako je vrlo malo njih pokazivalo slabu aktivnost. Stanice eritrocitne, megakariocitne i limfocitne linije bile su peroksidaza negativne. Stanice granulocitne linije bile su pozitivne na Sudan crnilo B, osim mijeloblasta. Neki monociti bili su pozitivni s nekoliko diseminiranih crnih granula, dok su drugi bili negativni. Stanice eritrocitne, megakariocitne i limfocitne linije bile su Sudan crnilom B negativne. Svi razvojni oblici granulocita reagirali su pozitivno na oksidaciju perjodnom kiselinom i bojenjem Shiffovim reagensom (PAS), osim mijeloblasta. Monociti su bili, ili negativni ili pozitivni. Neki limfociti pokazivali su nekoliko sitnih ili čak krupnih granula pozitivnih na PAS. Megakariociti su bili pozitivni na PAS, dok su sve stanice eritrocitne linije bile tim bojenjem negativne. Ustanovljena je sukladnost srednjih vrijednosti postotaka pozitivnih stanica obojenih trima citokemijskim bojenjima i broja granulocita koštane srži obojenih po May-Grünwald-Giemsi