Microscopy of bacterial translocation during small bowel obstruction and ischemia in vivo – a new animal model

BACKGROUND: Existing animal models provide only indirect information about the pathogenesis of infections caused by indigenous gastrointestinal microflora and the kinetics of bacterial translocation. The aim of this study was to develop a novel animal model to assess bacterial translocation and intestinal barrier function in vivo. METHODS: In anaesthetized male Wistar rats, 0.5 ml of a suspension of green fluorescent protein-transfected E. coli was administered by intraluminal injection in a model of small bowel obstruction. Animals were randomly subjected to non-ischemic or ischemic bowel obstruction. Ischemia was induced by selective clamping of the terminal mesenteric vessels feeding the obstructed bowel loop. Time intervals necessary for translocation of E. coli into the submucosal stroma and the muscularis propria was assessed using intravital microscopy. RESULTS: Bacterial translocation into the submucosa and muscularis propria took a mean of 36 ± 8 min and 80 ± 10 min, respectively, in small bowel obstruction. Intestinal ischemia significantly accelerated bacterial translocation into the submucosa (11 ± 5 min, p < 0.0001) and muscularis (66 ± 7 min; p = 0.004). Green fluorescent protein-transfected E. coli were visible in frozen sections of small bowel, mesentery, liver and spleen taken two hours after E. coli administration. CONCLUSIONS: Intravital microscopy of fluorescent bacteria is a novel approach to study bacterial translocation in vivo. We have applied this technique to define minimal bacterial transit time as a functional parameter of intestinal barrier function

MHD simulations of small and large scale dynamos

Isotropic homogeneous hydromagnetic turbulence is studied using numerical simulations at resolutions of up to 1024^3 meshpoints. It is argued that, in contrast to the kinematic regime, the nonlinear regime is characterized by a spectral magnetic power that is decreasing with increasing wavenumber, regardless of whether or not the turbulence has helicity. This means that the root-mean-square field strength converges to a limiting value at large magnetic Reynolds numbers. The total (magnetic and kinetic) energy spectrum tends to be somewhat shallower than k^{-5/3}, in agreement with the findings of other groups. In the presence of helicity, an inverse cascade develops, provided the scale separation between the size of the computational box and the scale of the energy carrying eddies exceeds a ratio of at least two. Finally, the constraints imposed by magnetic helicity conservation on mean-field theory are reviewed and new results of simulations are presented