2 research outputs found
Analysis and comparison of fragrant gene sequence in some rice cultivars
It is known that the fragrant trait in rice (Oryza sativa L.) is largely
controlled by fgr gene on chromosome 8 and it has been specified that the
existence of an 8 bp deletion and three single nucleotide polymorphism (SNP)
in exon 7 is effective on this trait. In this study, sequence alignment
analysis of fgr exon7 on chromosome 8 for 11 different fragrant and
non-fragrant cultivars revealed that 5 aromatic rice cultivars carried 3 SNPs
and 8 bp deletion in exon7 which terminates prematurely at a TAA stop codon.
However, 5 of the non-aromatics showed a sequence identical to the published
Nipponbare, being non-fragrant Japonica variety sequence. An exception among
them was Bejar, which had 8 bp deletion and 3SNPs but it was non-aromatic.
Sequencing can determine nucleotide alignment of a gene and give beneficial
information about gene function. In silico prediction showed proteins
sequences alignment of fgr gene for Khazar and Domsiah genotypes were
different. Betaine aldehyde dehydrogenase complete enzyme belongs to Khazar
non-fragrant genotype that has complete length and 503 amino acids while
non-functional BADH2 enzyme for Domsiah fragrant genotype has 251 amino acids
that result in accumulate 2-acetyl-1-pyrroline (2AP) and produces aroma in
fragrant genotypes