Bone Marrow Mesenchymal Cell Contribution in Maintenance of Periodontal Ligament Homeostasis

In general, remodeling phenomenon of the periodontal ligament (PDL) is occurring in all times. Thus, in the chapter, the word “maintenance” was used, and the chapter title is “Maintenance of Periodontal Ligament Homeostasis.” Our experimental data on the remodeling of the PDL with cell acceleration at the furcation area in this experimental model are recovered using the cells in situ and the bone marrow-derived cells (BMCs). BMC migration into the PDL tissues using green fluorescent protein (GFP) bone marrow-transplanted model mouse was examined. BMCs have abilities of cell migration and differentiation into tissues/organs in the body. The immunohistochemistry revealed that GFP-positive cells were detected in the PDL. GFP-positive cells were also positive to CD31, CD68, and Runx2 suggesting that fibroblasts differentiated into osteoclasts and tissue macrophages. In this way, Notch signaling involvement considered in our tentative examinations revealed that the experimentally induced periodontal polyp was examined; the cytological dynamics of the cells in granulation tissue are mainly from migration of undifferentiated mesenchymal cells of the bone marrow and differentiate into the tissue-specified cells. Furthermore, the data suggest that cell differentiation is due to Notch signaling

歯科矯正学的メカニカルストレスによるマウス歯周組織改造における細胞動態

The purpose of the study was to determine the cell dynamics in periodontal ligament in response to mechanical stress during orthodontic movement. Following Waldo’s method, a square sheet of rubber dam was inserted in between the first and second maxillary molars in 10 ddY mice leaving the stress load for 3 hours. After 3 days and at 1 week, cell count on pressure and tension sides of the periodontal ligament was determined. Furthermore, the type of cell present after mechanical stress was identified using GFP bone marrow transplantation mouse model. Immunohistochemistry was carried out at 0 min (immediately after mechanical stress), 24 hours, 1 week, 2 weeks and 6 months. Temporal changes in the expression of GFP-positive bone marrow derived cells were examined. Moreover, double immunofluorescent staining was performed to determine the type of cell in the periodontal ligament. Cell count on the tension side tremendously increased 3 days after mechanical stress. At 1 week, spindle and round cell count increased compared to the control group. These changes were observed on both tension and pressure sides. Cell count on pressure side at 3 days (22.11+/-13.98) and at 1 week (33.23+/-11.39) was higher compared to the control group (15.26+/-8.29). On the tension side, there was a significantly increased at 3 days (35.46+/-11.85), but decreased at 1 week (29.23+/-13.89) although it is still higher compared to the control group (AD+/-SD: 10.37+/-8.69). Using GFP bone marrow transplantation mouse model, GFP positive cell count increased gradually over time in 6 months. GFP positive cells were also positive to CD31, CD68 and Runx2 suggesting that fibroblasts differentiated into osteoclasts and tissue macrophages. In conclusion, mechanical stress during orthodontic movement promoted the increase in the number of cells in the periodontal ligament on both tension and pressure sides. The increase in the number of cells in the periodontal ligament is believed to be due to the migration and cell division of undifferentiated mesenchymal cells.2015博士（歯学）松本歯科大

Chapter 6 : Bone marrow masenchymal cell contribution in maintenance of periodontal ligament homeostasis.

In general, remodeling phenomenon of the periodontal ligament (PDL) is occurring in all times. Thus, in the chapter, the word “maintenance” was used, and the chapter title is “Maintenance of Periodontal Ligament Homeostasis.” Our experimental data on the remodeling of the PDL with cell acceleration at the furcation area in this experimental model are recovered using the cells in situ and the bone marrow-derived cells (BMCs).BMC migration into the PDL tissues using green Ěuorescent protein (GFP)bone　marrow-transplanted model mouse was examined. BMCs have abilities of　cell migration and diěerentiation into　tissues/organs in the body. The immunohistochemistry revealed that GFP-positive cells were detected in the PDL.GFP-positive cells were also positive to CD31, CD68, and Runx2 suggesting that ębroblasts diěerentiated into osteoclasts and tissue macrophages. In this　way, Notch signaling involvement considered in our tentative examinations revealed　that the experimentally induced periodontal polyp was examined; the cytological　dynamics of the cells in granulation tissue are mainly from migration of　undiěerentiated mesenchymal cells of the bone marrow and diěerentiate into the　tissue-specięed cells. Furthermore, the data suggest that cell diěerentiation is due to　Notch signaling.Edited by Thomas Heinbockel and Vonnie D.C. Shields : Published in London : IntechOpen, 2019

看護実践力育成のための発展型データベース・シミュレーション教材「Web 聖隷タウン」の開発 : 第1報

報告Reports　本稿は、保健師助産師看護師学校養成所指定規則の一部改正（2022 年４月１日施行）に伴う、本学看護学部のカリキュラム改革推進の一環として、2021 年度より検討が開始された看護実践力育成のための発展型データベース・シミュレーション教材「Web 聖隷タウン」の開発に関する第一報である。本学シミュレーション教育委員会は、「Web 聖隷タウン」の実用に向けた開発として、（１）地域の中の看護視点を育てるためのプロットの開発、（２）Web 聖隷タウン制作の技術的課題解決に向けた実用可能な制作環境の試用および検討を行っている。本稿では、「Web 聖隷タウン」の開発経緯と開発推進のための資源確保について、本教材の持つ機能、これまでの活動経過および今後の課題について報告する

Reconstruction from Two Projections with Prohibited Subregion : Algorithm, Switching Graph and Consistency

We consider the two projection tomography problem initiated by Lorentz, Gale and Ryser. Here we assume that a priori knowledge is available that presents a prohibited region for the original figure. We show that a modification of Ryser's reconstruction algorithm gives a solution. We then study the relation of the switching graph for the solution sets with and without the prohibited region. We apply our idea to get a better reconstruction figure imposing prohibited region artificially. Finally, we discuss the condition for a set to be prohibited region