Design sensitivity analysis of boundary element substructures

The ability to reduce or condense a three-dimensional model exactly, and then iterate on this reduced size model representing the parts of the design that are allowed to change in an optimization loop is discussed. The discussion presents the results obtained from an ongoing research effort to exploit the concept of substructuring within the structural shape optimization context using a Boundary Element Analysis (BEA) formulation. The first part contains a formulation for the exact condensation of portions of the overall boundary element model designated as substructures. The use of reduced boundary element models in shape optimization requires that structural sensitivity analysis can be performed. A reduced sensitivity analysis formulation is then presented that allows for the calculation of structural response sensitivities of both the substructured (reduced) and unsubstructured parts of the model. It is shown that this approach produces significant computational economy in the design sensitivity analysis and reanalysis process by facilitating the block triangular factorization and forward reduction and backward substitution of smaller matrices. The implementatior of this formulation is discussed and timings and accuracies of representative test cases presented

A preliminary summary of the observations of the 16 February 1984 solar flare (STIP Interval XV, 12-21 February 1984)

The solar flare on 16 Feb. 1984 (0900 UT) and the associated photon and particle emissions were perhaps the most interesting solar and interplanetary phenomena during STIP Interval XV, 12 to 21 Feb. 1984. The X-ray and microwave radio emissions, as observed from the Earth, were relatively weak and no optical flare was reported. However, the hard X-ray and low energy gamma-ray observations made with the Pioneer Venus Orbiter spacecraft behind the west limb of the Sun indicate that the flare was, in reality, very intense. There is evidence that the flare was located approx 40 deg behind the west limb of the Sun and hence, for instruments located near the Earth, the most intense parts of the X-ray and microwave radio sources were occulted by the photosphere. However, the effect of occultation on the metric type II, type III, and type IV and decimetric (type DCIM) radio sources appeared to be relatively small. Following the flare, a large increase in the counting rates was recorded by several ground level neutron monitors and energetic particle detectors located in interplanetary space. A preliminary analysis of the 16 Feb. 1984 flare observations follows

Lipopolysaccharide (LPS)-binding protein is carried on lipoproteins and acts as a cofactor in the neutralization of LPS.

Lipoproteins isolated from normal human plasma can bind and neutralize bacterial lipopolysaccharide (LPS) and may represent an important mechanism in host defense against gram-negative septic shock. Recent studies have shown that experimentally elevating the levels of circulating high-density lipoproteins (HDL) provides protection against death in animal models of endotoxic shock. We sought to define the components of HDL that are required for neutralization of LPS. To accomplish this we have studied the functional neutralization of LPS by native and reconstituted HDL using a rapid assay that measures the CD14-dependent activation of leukocyte integrins on human neutrophils. We report here that reconstituted HDL particles (R-HDL), prepared from purified apolipoprotein A-I (apoA-I) combined with phospholipid and free cholesterol, are not sufficient to neutralize the biologic activity of LPS. However, addition of recombinant LPS binding protein (LBP), a protein known to transfer LPS to CD14 and enhance responses of cells to LPS, enabled prompt binding and neutralization of LPS by R-HDL. Thus, LBP appears capable of transferring LPS not only to CD14 but also to lipoprotein particles. In contrast with R-HDL, apoA-I containing lipoproteins (LpA-I) isolated from plasma by selected affinity immunosorption (SAIS) on an anti-apoA-I column, neutralized LPS without addition of exogenous LBP. Several lines of evidence demonstrated that LBP is a constituent of LpA-I in plasma. Passage of plasma over an anti-apoA-I column removed more than 99% of the LBP detectable by ELISA, whereas 31% of the LBP was recovered by elution of the column. Similarly, the ability of plasma to enable activation of neutrophils by LPS (LBP/Septin activity) was depleted and recovered by the same process. Furthermore, an immobilized anti-LBP monoclonal antibody coprecipitated apoA-I. The results described here suggest that in addition to its ability to transfer LPS to CD14, LBP may also transfer LPS to lipoproteins. Since LBP appears to be physically associated with lipoproteins in plasma, it is positioned to play an important role in the neutralization of LPS

When is an alternative possibility robust?

According to some, free will requires alternative possibilities. But not any old alternative possibility will do. Sometimes, being able to bring about an alternative does not bestow any control on an agent. In order to bestow control, and so be directly relevant qua alternative to grounding the agent's moral responsibility, alternatives need to be robust. Here, I investigate the nature of robust alternatives. I argue that Derk Pereboom's latest robustness criterion is too strong, and I suggest a different criterion based on the idea that what agents need to be able to do is keep open the possibility of securing their blamelessness, rather than needing to directly ensure their own blamelessness at the time of decision

Indicators of replicative damage in equine tendon fibroblast monolayers

<p>Background: Superficial digital flexor tendon (SDFT) injuries of horses usually follow cumulative matrix microdamage; it is not known why the reparative abilities of tendon fibroblasts are overwhelmed or subverted. Relevant in vitro studies of this process require fibroblasts not already responding to stresses caused by the cell culture protocols. We investigated indicators of replicative damage in SDFT fibroblast monolayers, effects of this on their reparative ability, and measures that can be taken to reduce it.</p> <p>Results: We found significant evidence of replicative stress, initially observing consistently large numbers of binucleate (BN) cells. A more variable but prominent feature was the presence of numerous gammaH2AX (γH2AX) puncta in nuclei, this being a histone protein that is phosphorylated in response to DNA double-stranded breaks (DSBs). Enrichment for injury detection and cell cycle arrest factors (p53 (ser15) and p21) occurred most frequently in BN cells; however, their numbers did not correlate with DNA damage levels and it is likely that the two processes have different causative mechanisms. Such remarkable levels of injury and binucleation are usually associated with irradiation, or treatment with cytoskeletal-disrupting agents.</p> <p>Both DSBs and BN cells were greatest in subconfluent (replicating) monolayers. The DNA-damaged cells co-expressed the replication markers TPX2/repp86 and centromere protein F. Once damaged in the early stages of culture establishment, fibroblasts continued to express DNA breaks with each replicative cycle. However, significant levels of cell death were not measured, suggesting that DNA repair was occurring. Comet assays showed that DNA repair was delayed in proportion to levels of genotoxic stress.</p> <p>Conclusions: Researchers using tendon fibroblast monolayers should assess their “health” using γH2AX labelling. Continued use of early passage cultures expressing initially high levels of γH2AX puncta should be avoided for mechanistic studies and ex-vivo therapeutic applications, as this will not be resolved with further replicative cycling. Low density cell culture should be avoided as it enriches for both DNA damage and mitotic defects (polyploidy). As monolayers differing only slightly in baseline DNA damage levels showed markedly variable responses to a further injury, studies of effects of various stressors on tendon cells must be very carefully controlled.</p&gt

Results of known sustained-tone devices vs. known different-pitched-tone devices in improving pitch of "uncertain singers" in grades one and two

Thesis (Ed.M.)--Boston UniversityThe purpose of this study is to try to find out through an experiment if one type of device to improve pitch in children will work more effectively in less time than another. The two types of devices used in the experiment are "sustained-tone" and "different-pitched-tone" devices. Of the forty-six children used in the experiment, 48% had improved their pitch in singing enough to return to their classrooms and sing with the class correctly. Of the above group, 59% had been in the "sustained-tone" group. here were nine children, or 20% of the total number, who, at the end of the ten sessions, had completed only 50% of the devices of their respective groups. There were thirteen chilciren, or 28% of the group, who, at the end of the experiment, were still unsuccessful in pitch placement. There were two children, or 4% of the entire group, who showed no progress

Treatment of body forces in boundary element design sensitivity analysis

The inclusion of body forces has received a good deal of attention in boundary element research. The consideration of such forces is essential in the desgin of high performance components such as fan and turbine disks in a gas turbine engine. Due to their critical performance requirements, optimal shapes are often desired for these components. The boundary element method (BEM) offers the possibility of being an efficient method for such iterative analysis as shape optimization. The implicit-differentiation of the boundary integral equations is performed to obtain the sensitivity equations. The body forces are accounted for by either the particular integrals for uniform body forces or by a surface integration for non-uniform body forces. The corresponding sensitivity equations for both these cases are presented. The validity of present formulations is established through a close agreement with exact analytical results

Commuting, transitions and belonging: the experiences of students living at home in their first year at university

In this study, our cross-case analysis of students’ lives challenges the conventional home–university model of transition and highlights the importance of acknowledging the influence of this complex symbiotic relationship for students who attend university and live at home. We argue that as with stay-at-home holidays, or “staycations”, which are of such crucial importance to the tourism industry, so stay-at-home students or commuter students are vital to higher education and the term utilised here is “stayeducation”. Through the narratives of “stayeducation” students, we see how family and community aspects of students’ lives are far more significant than previously realised, and our study suggests that these heavily influence the development of a student sense of belonging. Drawing upon biographical narrative method, this paper introduces three first-year Business and Economics students enrolled at different universities in London and explores their journeys through their transition through home, school and early university life. Ways in which key themes play out in the transition stories of our students and the challenges and obstacles for the individual are drawn out through the cross-case analysis. Findings support the existing literature around gender, class and identity; however, new insights into the importance, for these students, of family, friendships and community are presented. Our work has implications for academic staff, those writing institutional policies, and argues for the creation of different spaces within which students can integrate into their new environment

Predictors of homelessness among families and single adults after exit from homelessness prevention and rapid re-housing programs: evidence from the Department of Veterans Affairs Supportive Services for Veteran Families Program

This article assesses the extent and predictors of homelessness among Veterans (both Veterans in families with children and single adults Veterans) exiting the Supportive Services for Veteran Families (SSVF) program, which is a nationwide homelessness prevention and rapid re-housing program geared primarily towards those experiencing crisis homelessness. Among rapid re-housing participants, 16% and 26% of single adult Veterans experienced an episode of homelessness at one and two years post-SSVF exit; the comparable figures at those follow-up times for Veterans in families were 9.4% and 15.5%, respectively. Relatively fewer single adult Veterans and Veterans in families receiving homelessness prevention services experienced an episode of homelessness at one and two years post-SSVF exit. Veteran-level characteristics, including age, gender, prior history of homelessness and recent engagement with VA healthcare were generally more salient predictors of homelessness following SSVF exit than variables measuring SSVF program factors, or community-level housing market conditions.U.S. Department of Veterans Affairs, National Center on Homelessness Among Veteran