18 research outputs found
The development of a single-step solid-phase immuno-extraction for morphine
No full textIf suitable adsorbents can be developed, solid-phase extraction using antibodies to selected analytes is potentially much more selective than liquid-liquid or solid-phase extraction. This preliminary study uses antisera to morphine immobilized on controlled-pore glass to investigate the feasibility of developing a selective extraction procedure. Columns are washed with 0.3% HCl and primed with phosphate-buffered saline. Phosphate-buffered saline of different pH and with different organic modifiers was investigated as elution solvent. The results indicate that morphine could be extracted from aqueous solution using an immune-extraction column and desorbed in a 1-mL fraction using 60% ethanol in phosphate-buffered saline of pH 2
Immunoaffinity-based extraction of phenylurea herbicides using mixed antibodies against isoproturon and chlortoluron.
No full textThe development of a single-step solid-phase immuno-extraction for morphine
No full textIf suitable adsorbents can be developed, solid-phase extraction using antibodies to selected analytes is potentially much more selective than liquid-liquid or solid-phase extraction. This preliminary study uses antisera to morphine immobilized on controlled-pore glass to investigate the feasibility of developing a selective extraction procedure. Columns are washed with 0.3% HCl and primed with phosphate-buffered saline. Phosphate-buffered saline of different pH and with different organic modifiers was investigated as elution solvent. The results indicate that morphine could be extracted from aqueous solution using an immune-extraction column and desorbed in a 1-mL fraction using 60% ethanol in phosphate-buffered saline of pH 2
A SENSITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR THE DETECTION OF STAPHYLOCOCCAL PROTEIN-A (SPA) PRESENT AS A TRACE CONTAMINANT OF MURINE IMMUNOGLOBULINS PURIFIED ON IMMOBILIZED PROTEIN-A
No full textASSESSMENT OF THE SUITABILITY OF COMMERCIALLY AVAILABLE SPA AFFINITY SOLID-PHASES FOR THE PURIFICATION OF MURINE MONOCLONAL-ANTIBODIES AT PROCESS SCALE
No full textA SENSITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR THE DETECTION OF STAPHYLOCOCCAL PROTEIN-A (SPA) PRESENT AS A TRACE CONTAMINANT OF MURINE IMMUNOGLOBULINS PURIFIED ON IMMOBILIZED PROTEIN-A
No full textDEVELOPMENT AND OPTIMIZATION OF AN IMMUNOAFFINITY-BASED SOLID-PHASE EXTRACTION FOR CHLORTOLURON
No full textASSESSMENT OF THE SUITABILITY OF COMMERCIALLY AVAILABLE SPA AFFINITY SOLID-PHASES FOR THE PURIFICATION OF MURINE MONOCLONAL-ANTIBODIES AT PROCESS SCALE
No full text
