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Not AvailableThe ability of NAD3 (subunit 3 of the NADH ubiquinone oxidoreductase) encoded by unedited nad3 (unad3) gene, when targeted into mitochondria, to cause ablation of tapetum was investigated. A gene construct was developed by cloning the coxIV presequence from yeast and the u-nad3 from safflower under the tapetum specific TA29 promoter to target U-NAD3 into mitochondria in the tapetal cell layer. Transgenic tobacco plants were realized with this construct using the Agrobacterium mediated transformation method and confirmed for the presence of the transgene. Among these plants, three were completely sterile and four were semi-sterile. The male sterile plants were morphologically similar to fertile plants, but the anthers were shorter and remained regressed below the stigma surface at anthesis. RT-PCR and western blotting confirmed anther specific expression of u-nad3 in these plants. Stable inheritance of the induced male sterility and its co-segregation with the introduced gene cassette was confirmed in the test cross progeny. This study demonstrates the proof-of-concept that u-nad3 could be used as a candidate to induce transgenic male sterility in plants.ICAR Cess fund and DB

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Not AvailableExpressed sequence tag (EST) databases offer opportunity for the rapid development of simple sequence repeat (SSR) markers in crops. Sequence assembly and clustering of 57 895 ESTs of castor bean resulted in the identification of 10 960 unigenes (6459 singletons and 4501 contigs) having 7429 SSRs. On an average, the unigenes contained 1 SSR for every 1.23 kb of unigene sequence. The identified SSRs mostly consisted of dinucleotide (62.4%) and trinucleotide (33.5%) repeats. The AG class was the most common among the dinucleotide motifs (68.9%), whereas the AAG class (25.9%) was predominant among the trinucleotide motifs. A total of 611 primer pairs were designed for the SSRs, having repeat length more than or equal to 20 nucleotides, of which a set of 130 markers were tested and 92 of these yielding robust amplicons were analyzed for their utility in genetic purity assessment of castor bean hybrids. Nine markers were able to detect polymorphism between the parental lines of nine commercial castor bean hybrids (DCH-32, DCH-177, DCH-519, GCH-2, GCH-4, GCH-5, GCH-6, GCH-7, and RHC-1), and their utility in genetic purity testing was demonstrated. These novel EST–SSR markers would be a valuable addition to the growing molecular marker resources that could be used in genetic improvement programmes of castor bean.Institut

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Not AvailableThe present study was undertaken with the objective to determine the degree of association between yield and it`s component characters and their direct, indirect effects on seed yield in sesame (Sesamum indicum L.). 41 genotypes were evaluated for identifying their efficiency with respect to 10 quantitative characters. Character association studies revealed significant positive association of seed yield per plant with plant height, number of branches per plant, first capsule height, capsules per plant, and 1000-seed weight. This indicated the possibility of simultaneous selection of all these characters for yield improvement. A critical path analysis revealed that the number of capsules per plant, diameter of stem, capsule length, number of branches per plant and plant height directly affected the seed yield per plant. This indicated that direct selection for yield improvement via these traits would be possibleNot Availabl

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Not AvailableAssessment of genetic purity of two sunflower (Helianthus annuus L.) hybrids using sequence characterized amplified region (SCAR) markersNot Availabl

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Not AvailableSterility in the universally exploited PET1-CMS system of sunflower is associated with the expression of orfH522, a novel mitochondrial gene. Definitive evidence that ORFH522 is directly responsible for male sterility is lacking. To test the hypothesis that ORFH522 is sufficient to induce male sterility, a set of chimeric constructs were developed. The cDNA of orfH522 was cloned in-frame with yeast coxIV pre-sequence, and was expressed under tapetum-specific promoter TA29 (construct designated as TCON). For developing control vectors, orfH522 was cloned without the transit peptide under TA29 promoter (TON) or orfH522 was cloned with or without transit peptide under the constitutive CaMV35S promoter (SCOP and SOP). Among several independent transformants obtained with each of the gene cassettes, one third of the transgenics (6/17) with TCON were completely male sterile while more than 10 independent transformants obtained with each of the control vectors were fertile. The male sterile plants were morphologically similar to fertile plants, but had anthers that remained below the stigmatic surface at anthesis. RT-PCR analysis of the sterile plants confirmed the anther-specific expression of orfH522 and bright-field microscopy demonstrated ablation of the tapetal cell layer. Premature DNA fragmentation and programmed cell death was observed at meiosis stage in the anthers of sterile plants. Stable transmission of induced male sterility trait was confirmed in test cross progeny. This constitutes the first report at demonstrating the induction of male sterility by introducing orfH522 gene that could be useful for genetic engineering of male sterility.Not Availabl

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Not AvailableWith the aim of developing additional genomic resources in safflower, a set of 41,011 ESTs of safflower were mined for the presence of SSRs. 18,773 SSR containing ESTs (SSR-ESTs) were identified and were analyzed to remove redundant sequences leading to identification of 8,810 nonredundant SSR-ESTs (categorized into 6104 singletons and 2,706 contigs) having 13,085 non-redundant SSRs. The average number of non-redundant SSRs per ESTwas 0.32 and they predominantly consisted of dinucleotide (57.7 %), and trinucleotide (37.7%) repeat motifs. 500 primer pairs were designed for the non-redundant EST-SSRs of which, 151 were tested. 60 markers which gave robust amplicons, were validated in a set of 19 Carthamus lines. A subset of EST-SSR markers, having average polymorphism information content (PIC) ≥0.4 could precisely elucidate the pedigree relatedness among these lines. Further, these markers exhibited high cross-species transferability to five other wild species of Carthamus. The markers reported here would be a valuable addition to existing safflower marker resources aiding in hastening its improvement.Not Availabl

Hybrid Technology–a new vista in pigeonpea breeding

Once designated as an ‘orphan crop’ to now being crowned as a mainstream ‘commercial crop’, pigeonpea has evolved over the decades as lifeline for millions of resource poor farmers in the semi-arid tropics, where it is cultivated for both subsistence and commercial purpose.Pigeonpea [Cajanus cajan (L.)] is the sixth most important legume crop, grown predominantly in the tropical and sub-tropical regions of Asia, Africa and Latin America. India is considered as the center of origin of pigeonpea (Van der Maesen, 1980) because of its natural genetic variability available in the local germplasm and the presence of its wild relatives in the country..

Genetic variability and correlation in pigeonpea genotypes

Forty nine pigeon pea genotypes were evaluated at Agricultural Research Station Tandur during kharif 2015-16. Genotypes were grouped into six clusters based on Mahalonobis D2 statistics. Days to maturity contributed to maximum genetic divergence followed by days to 50% flowering. Maximum inter cluster distance was observed between clusters II and VI and intra cluster distance in cluster I and II. Genotypes in cluster V recorded highest mean values for number of secondary branches/plant and number of pods/plant and seed yield. Broad sense heritability estimates were highest for days to maturity and days to 50% flowering. Significant and positive genotypic and phenotypic correlation was observed between seed yield and number of pods/plant and number of secondary branches/plant. The range of GCV observed was 4.55 to 22.07% for the traits under study indicating the extent of variability present among the pigeon pea genotypes. Path coefficient analysis revealed that days to maturity exhibited maximum direct effect followed by number of pods/plant

Genotyping-by-sequencing of three mapping populations for identification of candidate genomic regions for resistance to sterility mosaic disease in pigeonpea

Sterility mosaic disease (SMD) is one of the serious production constraints that may lead to complete yield loss in pigeonpea. Three mapping populations including two recombinant inbred lines and one F2, were used for phenotyping for SMD resistance at two locations in three different years. Genotyping-by-sequencing approach was used for simultaneous identification and genotyping of SNPs on above mentioned populations. In total, 212,464, 89,699 and 64,798 SNPs were identified in ICPL 20096 × ICPL 332 (PRIL_B), ICPL 20097 × ICP 8863 (PRIL_C) and ICP 8863 × ICPL 87119 (F2) respectively. By using high-quality SNPs, genetic maps were developed for PRIL_B (1,101 SNPs; 921.21 cM), PRIL_C (484 SNPs; 798.25 cM) and F2 (996 SNPs; 1,597.30 cM) populations. The average inter marker distance on these maps varied from 0.84 cM to 1.65 cM, which was lowest in all genetic mapping studies in pigeonpea. Composite interval mapping based QTL analysis identified a total of 10 QTLs including three major QTLs across the three populations. The phenotypic variance of the identified QTLs ranged from 3.6 to 34.3%. One candidate genomic region identified on CcLG11 seems to be promising QTL for molecular breeding in developing superior lines with enhanced resistance to SMD

Indel-seq: a fast-forward genetics approach for identification of trait-associated putative candidate genomic regions and its application in pigeonpea (Cajanus cajan)

Identification of candidate genomic regions associated with target traits using conventional mapping methods is challenging and time-consuming. In recent years, a number of single nucleotide polymorphism (SNP)-based mapping approaches have been developed and used for identification of candidate/putative genomic regions. However, in the majority of these studies, insertion–deletion (Indel) were largely ignored. For efficient use of Indels in mapping target traits, we propose Indel-seq approach, which is a combination of whole-genome resequencing (WGRS) and bulked segregant analysis (BSA) and relies on the Indel frequencies in extreme bulks. Deployment of Indel-seq approach for identification of candidate genomic regions associated with fusarium wilt (FW) and sterility mosaic disease (SMD) resistance in pigeonpea has identified 16 Indels affecting 26 putative candidate genes. Of these 26 affected putative candidate genes, 24 genes showed effect in the upstream/downstream of the genic region and two genes showed effect in the genes. Validation of these 16 candidate Indels in other FW- and SMD-resistant and FW- and SMD-susceptible genotypes revealed a significant association of five Indels (three for FW and two for SMD resistance). Comparative analysis of Indel-seq with other genetic mapping approaches highlighted the importance of the approach in identification of significant genomic regions associated with target traits. Therefore, the Indel-seq approach can be used for quick and precise identification of candidate genomic regions for any target traits in any crop species