UHPLC-ESI/TOFMS Determination of Salicylate-like Phenolic Gycosides in Populus tremula Leaves

Associations of salicylate-like phenolic glycosides (PGs) with biological activity have been reported in Salix and Populus trees, but only for a few compounds, and in relation to a limited number of herbivores. By considering the full diversity of PGs, we may improve our ability to recognize genotypes or chemotype groups and enhance our understanding of their ecological function. Here, we present a fast and efficient general method for salicylate determination in leaves of Eurasian aspen that uses ultra-high performance liquid chromatography-electrospray ionization/time-of-flight mass spectrometry (UHPLC-ESI/TOFMS). The time required for the liquid chromatography separations was 13.5 min per sample, compared to around 60 min per sample for most HPLC protocols. In leaf samples from identical P. tremula genotypes with diverse propagation and treatment histories, we identified nine PGs. We found the compound-specific mass chromatograms to be more informative than the UV-visible chromatograms for compound identification and when quantitating samples with large variability in PG content. Signature compounds previously reported for P. tremoloides (tremulacin, tremuloidin, salicin, and salicortin) always were present, and five PGs (2'-O-cinnamoyl-salicortin, 2'-O-acetyl-salicortin, 2'-O-acetyl-salicin, acetyl-tremulacin, and salicyloyl-salicin) were detected for the first time in P. tremula. By using information about the formic acid adduct that appeared for PGs in the LTQ-Orbitrap MS environment, novel compounds like acetyl-tremulacin could be tentatively identified without the use of standards. The novel PGs were consistently either present in genotypes regardless of propagation and damage treatment or were not detectable. In some genotypes, concentrations of 2'-O-acetyl-salicortin and 2'-O-cinnamoyl-salicortin were similar to levels of biologically active PGs in other Salicaceous trees. Our study suggests that we may expect a wide variation in PG content in aspen populations which is of interest both for studies of interactions with herbivores and for mapping population structure

A factor analytic study of symptoms in acute schizophrenia

The three-syndrome model of schizophrenic phenomenology has been well established in chronic illness. We report an attempt to replicate this concept in a sample of acutely unwell schizophrenic patients. Factor analysis was performed using PSE data collected within a week of admission from 114 patients who met DSMIII(R) diagnostic criteria for schizophrenia. Four main factors accounted for 58% of the variance. The first two factors closely resembled Liddle's disorganisation and psychomotor poverty syndromes. Hallucinations and delusions loaded separately under the third and fourth factors. To examine the validity of these four syndromes, the relationships between factor scores and other clinical data were examined. The disorganisation syndrome was associated with a history of multiple past admissions and a longer lifetime duration of in-patient treatment.link_to_subscribed_fulltex

Departure from linearity of fibre Bragg grating temperature coefficients

We have measured the Bragg wavelength shift of three gratings from -30degreesC to +80degreesC. The observed deviation from linearity is a quadratic function of temperature, arising from the non-linear thermo-optic coefficient of silica glass

A fibre optic microsensor for measurement of temperature dynamics in gas turbine systems

The use of computational fluid dynamics (CFD) to model the temperature and pressure distributions which drive complex thermodynamic processes in gas turbine systems contributes to more cost efficient turbine design and development. However, experimental measurements are still required for validation of full-field CFD models, particularly in regions of highly unsteady flow where model stability is most severely tested. THis work concerns the development of fiber optic temperature and pressure microsensors for measurements combining high spatial resolution and high bandwidth in unsteady gas flows. The objective of the work presented in this paper was to measure gas total temperature in a large- scale turbomachinery test-rig at DERA Pyestock, known as the Isentropic Light Piston Facility. We have accordingly designed a dual sensor system, in which one of the elements is heated, so that gas total temperature can be measured independently of the convective heat transfer coefficient. While such dual element probes based on thin-film resistance gauges have been developed previously for aerodynamic studies, this is the first report of their development using optical sensors. ©2003 Copyright SPIE - The International Society for Optical Engineering