El Diario de Pontevedra : periódico liberal: Ano LI Número 15301 - 1937 novembro 2

Fig. S1. Viability of FaDu and Cal27 cells at low concentrations of methylglyoxal. Assessment of the cytotoxic effect of MG at low concentration in a colony-forming assay. (TIFF 92 kb

Perfiles paleokarsticos en el techo de la unidad intermedia del mioceno de la cuenca de Madrid

An intra-Vallesian (Upper Miocene) paleokarst developed at the top of the Intermediate Miocene Unit in the continental Madrid basin is recognized. This paleokarst is a early shallow, tabular-shaped karst that shows a marked control by the depositional facies pattern and lithologies. By integrating morphological, petrological and geochemica1 data, three hydrogeological or environmental zones have been established throughout the paleokarstic profiles: (i) a vadose zone, characterized by vertically elongated caves and discontinuous speleothems and vadose cements (ii) a 3-7 m thick water table fringe, characterized by the wide development of stratiform breccia bodies, the superimposition of both vadose and phreatic features, and the lowest Fe and Mn contents in host-rock carbonates; and (iii) a phreatic zone characterized by an increase of 6I3C values and the predominance of phreatic cementation. The paleogeographic reconstruction for the intra-Vallesian paleokarst using paleokarstic profiles reveals relative topographic highs to the north and topographic lows to the south drawing the paleokarst landscape.<br><br>En el techo de la Unidad Intermedia del Mioceno de la Cuenca de Madrid se ha desarrollado un paleokarst temprano, somero y de forma tabular que muestra un marcado control litol&#243;gico y del dispositivo de facies deposicionales en su g&#233;nesis. Integrando criterios geomorfol&#243;gicos, petrogr&#225;ficos y geoqu&#237;micos se ha establecido una zonaci&#243;n hidrogeol&#243;gica en los perfiles paleok&#225;rsticos estudiados, diferenci&#225;ndose: (i) una zona vadosa caracterizada por la existencia de cavidades alargadas en la vertical tapizadas discontinuamente por espeleotemas y otros cementos vadosos; (ii) una franja de oscilaci&#243;n del nivel fre&#225;tico de unos 3-7 metros de espesor, caracterizado por el desarrollo extensivo de cuerpos brechoides estratiformes, la yuxtaposici&#243;n de cementos vadosos y fre&#225;ticos y los contenidos m&#225;s bajos en Fe y Mn en el material encajante, y (iii) una zona fre&#225;tica caracterizada por un aumento en los valores de 613C y el predominio de la cementaci&#243;n fre&#225;tica. La correlaci&#243;n de los perfiles paleok&#225;rticos revela una paleogeograf&#237;a para el techo de la Unidad Intermedia con un paisaje topogr&#225;ficamente descendente de norte a sur en la cuenca para el Vallesiense

Additional file 7: of Glyoxalase 1 expression is associated with an unfavorable prognosis of oropharyngeal squamous cell carcinoma

Table S5. Univariate analysis of distinct risk factors for progression-free and disease-specific survival. (DOCX 72 kb

Veldaangeleenthede en beweging van wild in die Kalahari - Gemsbokpark

Veldaangeleenthede en beweging van wild in die Kalahari - Gemsbokpar

Additional file 3: Figure S2. of Impaired aldehyde dehydrogenase 1 subfamily member 2A-dependent retinoic acid signaling is related with a mesenchymal-like phenotype and an unfavorable prognosis of head and neck squamous cell carcinoma

ALDH1A2 expression in HNSCC cell lines and morphological phenotype upon inhibition of ALDH1A2-RAR signaling. Western blot analysis with whole cell lysate demonstrates protein expression of ALDH1A2 and key regulators of RA signaling in UMSCC-17B, Detroit562, FaDu, Cal27 and SCC25 cells (A), and ALDH1A2 protein levels in newly established HNSCC cell lines from Lausanne (B). Detection of β-Actin served as control for quantity and quality of protein lysates. Relative RA levels were determined by HPLC analysis with whole cell lysate of untreated (C), and DMSO-treated control or WIN18.446-treated Cal27 and FaDu cells (D). Bars represent mean values ¹ SD of three independent replicates. Graphs indicate relative survival fraction of Cal27 and FaDu cells, which were treated with the indicated concentration of Adapalene (E) or Fenretinide (F). Data represent mean values ¹ SD of three independent replicates. (TIF 301 kb

Additional file 1: Figure S1. of Impaired aldehyde dehydrogenase 1 subfamily member 2A-dependent retinoic acid signaling is related with a mesenchymal-like phenotype and an unfavorable prognosis of head and neck squamous cell carcinoma

Association between subgroups with high or low protein expression of CRABP2 (A), FABP5 (B), RARα (C), RARβ (D) or PPARα/δ (E) and overall survival of OPSCC patients was assessed by univariate Kaplan-Meier analysis. (F) Kaplan-Meier analysis demonstrates overall survival probability for subgroups with indicated staining patterns for ALDH1A2, CRABP2 and FABP5. Number at risk indicates the total amount of patients per subgroup, which were alive and not censored at the indicated time points and were considered to calculate the overall survival probability. P values were calculated by log-rank tests. (TIF 1257 kb

Additional file 5: Figure S4. of Impaired aldehyde dehydrogenase 1 subfamily member 2A-dependent retinoic acid signaling is related with a mesenchymal-like phenotype and an unfavorable prognosis of head and neck squamous cell carcinoma

(A) Representative phase contrast pictures of FaDu, Detroit562 and UMSCC-17B cells, which were treated with DMSO, 3 μM WIN18.446 or 3 μM BMS493 for four days. (B) Representative fluorescent pictures of FaDu and Detroit562 cells, which were treated as described in (A), and were stained with Phalloidin-Alexa488 (green signal). Nuclear staining was done with H33342 (blue signal). Migration of Detroit562 (C) and FaDu cells (D), which were treated with DMSO (white bars), 3 μM WIN18.446 (grey bars) or 3 μM BMS493 (black bars), in a scratch wounding assay was determined by the relative gap closure at the indicated time points. * p value ≤ 0.05. (TIF 4129 kb

Co-Immunofluorescence staining with anti-vimentin (green) and anti-pan cytokeratin (red) of an ACC sample (A) with no overexpression of vimentin, B) Co-Immunofluorescence staining with anti-vimentin (green) and anti-e-cadherin (red) of an ACC sample without vimentin expression but e-cadherin overexpression, C) with moderate vimentin overexpression marked by green and detectable e-cadherin expression marked by red arrows, D) high vimentin expression in almost all tumor cells.

<p>Co-Immunofluorescence staining with anti-vimentin (green) and anti-pan cytokeratin (red) of an ACC sample (A) with no overexpression of vimentin, B) Co-Immunofluorescence staining with anti-vimentin (green) and anti-e-cadherin (red) of an ACC sample without vimentin expression but e-cadherin overexpression, C) with moderate vimentin overexpression marked by green and detectable e-cadherin expression marked by red arrows, D) high vimentin expression in almost all tumor cells.</p

Representative microscopic images of tumor samples from 2 patients with an ACC.

<p>A) shows a tumor sample with a high SOX2 expression using immunohistochemistry (in 4x and 40x magnification) and B) an almost absent expression of ki67. C) shows a tumor sample with no detectable SOX2 expression and D) with elevated levels of ki67.</p

A) solid growth patterns of ACC show a trend towards a worse clinical outcome as assessed by Kaplan-Meier survival analysis, B) extra-parotideal ACCs show significantly worse overall survival C) SOX2 positive ACCs show prolonged overall survival compared to tumors with a loss of SOX2.

<p>M+ positive were excluded. Fig 1A post-hoc power: 30.7%, Fig 1B post-hoc power: 49.9%, Fig 1C post-hoc power: 15.8%.</p