Additional file 1: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Figure S1. Example of gating used in data analysis. The isotype (and FMO) was used to set the negative gate, and then the percentage of positive cells was taken as the percentage above this gate. Separate healthy and sepsis isotypes were used (Figure S1a and b respectively). Figure S1c shows an example of MFI signalling in healthy and sepsis isotypes, and healthy, sepsis survivor and sepsis non-survivor samples. (DOCX 441 kb

Additional file 15: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Figure S10. Serum level comparison. Comparison of levels of serum PD-1 and PD-L1 between patients with sepsis and healthy controls. (DOCX 175 kb

Additional file 10: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Figure S5. CD4+ T cell percentage positivity. Comparison of the expression levels as determined by percentage positivity of PD-1, PD-L1 and PD-L2 on CD4+ T cell subsets (CD27+ and CD27-) between patients with sepsis and healthy controls. (DOCX 465 kb

Additional file 17: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Table S6. sPD-1/sPD-L1 studies in sepsis. Summary of previous studies measuring soluble serum PD-1 and PD-L1 (sPD-1; sPD-L1) levels in sepsis. (DOCX 12 kb

Additional file 4: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Table S3. Proportion of positive B and T cells. Proportion of B and CD4+ T cells that express PD-1, PD-L1 and PD-L2 in patients with sepsis compared to healthy controls. (DOCX 12 kb

Additional file 14: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Figure S9. Comparison by survival status. Comparison of PD-1, PD-L1 and PD-L2 expression by B and CD4+ T cells between sepsis survivors and non-survivors. (DOCX 278 kb

Additional file 16: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Figure S11. Serum versus cell surface expression. Serum levels of PD-1 and PD-L1 are plotted against cell surface expression levels on B cells and CD4+ T cells. (DOCX 286 kb

Additional file 5: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Table S4. Proportion of positive cells by CD27 expression status. Proportion of CD27+ B cells, CD27- B cells, CD27+ CD4+ T cells and CD27- CD4+ T cells that express PD-1, PD-L1 and PD-L2 in patients with sepsis compared to healthy controls. (DOCX 12 kb

Additional file 3: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Table S2. Survival status, microbiology results and nosocomial infection site in patients who developed nosocomial infection. (DOCX 12 kb

Additional file 9: of Lymphocyte subset expression and serum concentrations of PD-1/PD-L1 in sepsis - pilot study

Figure S4. CD4+ T cell subset MFI. Comparison of expression of PD-1, PD-L1 and PD-L2 as determined by MFI on CD4+ T cell subsets (CD27+ and CD27-) between patients with sepsis and healthy controls. (DOCX 257 kb