Guía de trabajos prácticos de laboratorio de Microbiología

Guide des Travaux Pratiques de Microbiologieinfo:eu-repo/semantics/publishe

Fundamentos y aplicaciones de la Microbiología

Fondements et applications de la Microbiologieinfo:eu-repo/semantics/publishe

The relationship between pellet size and production of Mn (II) peroxidase by Phanerochaete chrysosporium in submerged culture

info:eu-repo/semantics/publishe

Production of a high level of laccase by submerged fermentation at 120-L scale of Cerrena unicolor C-139 grown on wheat bran

Submerged fermentation in a stirred bioreactor of the white rot fungus Cerrena unicolor C- 139 was done at a 120-L scale in the presence of wheat bran as a cheap lignocellulosic substrate for fungus growth and laccase production. Enzyme monitoring showed that laccase production started after 2 days of cultivation, attaining a maximum activity of 416.4 U·mL-1 at day 12 of fermentation. After treatment of culture liquid by successive micro- and ultrafiltration (5 kDa), a liquid concentrate containing 22203176 units of laccase was obtained. Obtaining large amount of laccase is essential for various industrial applications, including detoxification of industrial effluents, textile and petrochemical industries, polymer synthesis, bioremediation of contaminated area, stabilization of beverages, production of cosmetics, manufacture of anti-cancer drugs, and nanobiotechnology. The cultivation method and the fungal strain used here provided a substantial amount of enzyme produced at a price lower than 0.01 € cent/unit enzyme.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

In situ localization of manganese peroxidase production in mycelial pellets of Phanerochaete chrysosporium.

The ultrastructure of Phanerochaete chrysosporium hyphae from pellets in submerged liquid cultures was investigated in order to learn more about the interrelation between fungal architecture and manganese peroxidase (MnP) production. At day 2 of cultivation, some subapical regions of hyphae in the outer and middle zones of the pellet initiated differentiation into intercalary thick-walled chlamydospore-like cells of about 10 micro m diameter. At the periphery of the cytoplasm of these cells, a large number of mitochondria and Golgi-like vesicles were observed. The sites of MnP production were localized at different stages of cultivation by an immunolabelling procedure. The immunomarker of MnP was mainly concentrated in the chlamydospore-like cells and principally distributed in Golgi-like vesicles located at the periphery of the cytoplasm. The apices of hyphae in the outer layer of the pellets were apparently minor sites of MnP production. Maximal MnP release into the culture supernatant coincided with apparent autolysis of the chlamydospore-like cells. Production of extracellular autolytic chitinase and protease coincided with the disappearance of these structures from the pellets. The chlamydospore-like cells observed in the mycelial pellets of P. chrysosporium could be metabolically active entities operating as an enzyme reservoir, delivering their content into the surrounding medium possibly by an enzyme-mediated autolytic process.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe

Immobilized Coriolopsis sp. laccase for continuous elimination and transformation of phenolic micropollutants

The evaluation of continuous elimination and transformation of toxic endocrine disruptors micropollutants (MPs) (Bisphenol A, Nonylphenol [NP] and Triclosan [TCS]) using immobilized laccase of Coriolopsis sp. showed the high potential of this laccase as a bio-agent for the elimination and transformation process of selected MPs. A toxicology test using Escherichia coli has shown that toxicity completely disappeared after transformation and elimination of Bisphenol A, while in cases of NP and TCS 95% and 90% toxicity, respectively, disappeared. As compared to initial activity, immobilized laccase activity remained practically constant at the end of several runs of elimination and transformation processes of MPs. This feature is promising for the use of immobilized Coriolopsis sp. laccase in a future industrial depollution process.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Fungi autochthonous from the tropical forest of Colombia for dye decolourisation and ligninolytic enzymes production

info:eu-repo/semantics/publishe

Removal of two organophosphate pesticides by a bacterial consortium immobilized in alginate or tezontle.

In order to remove methyl-parathion (MP) and tetrachlorvinphos (TCF), a bacterial consortium was immobilized with two supports consisting of alginate beads or stones of tezontle colonized by biofilm. Removal kinetics were recorded for suspended and immobilized consortium using a mineral salt medium supplemented with MP and TCF at 25mg/L and with 0.1% (w/v) glucose as a co-substrate. The viability of the consortium cultivated in suspension was maintained for 6 days, whereas the viability of the consortium immobilized in alginate and tezontle supports was maintained for up to 11 and 13 days, respectively. Growth was enhanced when using glucose as a co-substrate. The percentage of MP removed was significantly higher (alpha=0.05) when consortium was immobilized in alginate beads and biofilm on tezontle as compared to suspension culture.Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe

OXEROM :the potential of white rot fungi oxidative enzymes systems for removal of organic micropollutants in urban and industrial wastewaters: Rapport semestriel N°3

info:eu-repo/semantics/nonPublishe

Production of Cerrena unicolor laccase and its insolubilization for transformation of phenolics content in red and white Porto wines

info:eu-repo/semantics/nonPublishe