An Integrated Analysis of miRNA and mRNA Expressions in Non-Small Cell Lung Cancers

Using DNA microarrays, we generated both mRNA and miRNA expression data from 6 non-small cell lung cancer (NSCLC) tissues and their matching normal control from adjacent tissues to identify potential miRNA markers for diagnostics. We demonstrated that hsa-miR-96 is significantly and consistently up-regulated in all 6 NSCLCs. We validated this result in an independent set of 35 paired tumors and their adjacent normal tissues, as well as their sera that are collected before surgical resection or chemotherapy, and the results suggested that hsa-miR-96 may play an important role in NSCLC development and has great potential to be used as a noninvasive marker for diagnosing NSCLC. We predicted potential miRNA target mRNAs based on different methods (TargetScan and miRanda). Further classification of miRNA regulated genes based on their relationship with miRNAs revealed that hsa-miR-96 and certain other miRNAs tend to down-regulate their target mRNAs in NSCLC development, which have expression levels permissive to direct interaction between miRNAs and their target mRNAs. In addition, we identified a significant correlation of miRNA regulation with genes coincide with high density of CpG islands, which suggests that miRNA may represent a primary regulatory mechanism governing basic cellular functions and cell differentiations, and such mechanism may be complementary to DNA methylation in repressing or activating gene expression

Enhancing the resilience of the power system to accommodate the construction of the new power system: key technologies and challenges

The increasingly frequent extreme events pose a serious threat to the resilience of the power system. At the same time, the power grid is transforming into a new type of clean and low-carbon power system due to severe environmental issues. The system shows strong randomness with a high proportion of renewable energy, which has increased the difficulty of maintaining the safe and stable operation of the power system. Therefore, it is urgent to improve the resilience of the new power system. This paper first elaborates on the concept of power system resilience, listing the characteristics of new power systems and their impact on grid resilience. Secondly, the evaluation methods for resilient power grids are classified into two categories, and measures to improve the resilience of the new power system are reviewed from various stages of disasters. Then, the critical technologies for improving the resilience of the new power system are summarized. Finally, the prospective research directions for new power system resilience enhancement are expounded

Intracoronary artery retrograde thrombolysis combined with percutaneous coronary interventions for ST-segment elevation myocardial infarction complicated with diabetes mellitus: A case report and literature review

BackgroundThe management of a large thrombus burden in patients with acute myocardial infarction and diabetes is still a worldwide problem.Case presentationA 74-year-old Chinese woman presented with ST-segment elevation myocardial infarction (STEMI) complicated with diabetes mellitus and hypertension. Angiography revealed massive thrombus formation in the mid-segment of the right coronary artery leading to vascular occlusion. The sheared balloon was placed far from the occlusion segment and urokinase (100,000 u) was administered for intracoronary artery retrograde thrombolysis, and thrombolysis in myocardial infarction (TIMI) grade 3 blood flow was restored within 7 min. At last, one stent was accurately implanted into the culprit’s vessel. No-reflow, coronary slow flow, and reperfusion arrhythmia were not observed during this process.ConclusionIntracoronary artery retrograde thrombolysis (ICART) can be effectively and safely used in patients with STEMI along with diabetes mellitus and hypertension, even if the myocardial infarction exceeds 12 h (REST or named ICART ClinicalTrials.gov number, ChiCTR1900023849)

Effect of Solution Treatment and Aging Treatment on Mechanical Properties of 2060 Al-Li Alloy and Process Multi-Objective Optimization

Solution and aging heat treatment are effective means to improve the mechanical properties of Al-Li alloys. The solution treatment and aging treatment tests of 2060 Al-Li alloy were carried out. The yield strength, tensile strength, elongation, Vickers hardness, and microstructure of specimens after heat treatment were obtained by the tensile test at room temperature, Vickers hardness test, SEM analysis, TEM analysis, and EDS analysis. The effects of solution and aging heat treatment parameters on mechanical properties of 2060 Al-Li alloy were analyzed by response surface model and test results. The results show that sufficient solution can make the Cu-rich second phase of the alloy continuously dissolves into the aluminum matrix, and consequently obtain the supersaturated solid solution, the insoluble second phase is mainly θ’ (Al2Cu) phase, T(Al2Cu2Mg3) phase, and S’ (Al2CuMg) phase. The strength and hardness of the alloy are improved, but the ductility worsens with the degree of solution treatment enhances. With the increase of aging temperature and aging time, the strength and hardness of the alloy increase, but the elongation decreases. Taking tensile strength, elongation, and Vickers hardness of the alloy as the optimization objectives, the NSGA-II multi-objective optimization algorithm was used to optimize the process parameters of solution and aging heat treatment, and the heat treatment experiment was carried out. The optimization results show that the best mechanical properties of the alloy with matching strength and toughness can be obtained under the solution treatment at 466 °C/60 min and aging treatment at 185 °C/13 h

Effect of Solution Treatment and Aging Treatment on Mechanical Properties of 2060 Al-Li Alloy and Process Multi-Objective Optimization

Solution and aging heat treatment are effective means to improve the mechanical properties of Al-Li alloys. The solution treatment and aging treatment tests of 2060 Al-Li alloy were carried out. The yield strength, tensile strength, elongation, Vickers hardness, and microstructure of specimens after heat treatment were obtained by the tensile test at room temperature, Vickers hardness test, SEM analysis, TEM analysis, and EDS analysis. The effects of solution and aging heat treatment parameters on mechanical properties of 2060 Al-Li alloy were analyzed by response surface model and test results. The results show that sufficient solution can make the Cu-rich second phase of the alloy continuously dissolves into the aluminum matrix, and consequently obtain the supersaturated solid solution, the insoluble second phase is mainly θ’ (Al2Cu) phase, T(Al2Cu2Mg3) phase, and S’ (Al2CuMg) phase. The strength and hardness of the alloy are improved, but the ductility worsens with the degree of solution treatment enhances. With the increase of aging temperature and aging time, the strength and hardness of the alloy increase, but the elongation decreases. Taking tensile strength, elongation, and Vickers hardness of the alloy as the optimization objectives, the NSGA-II multi-objective optimization algorithm was used to optimize the process parameters of solution and aging heat treatment, and the heat treatment experiment was carried out. The optimization results show that the best mechanical properties of the alloy with matching strength and toughness can be obtained under the solution treatment at 466 °C/60 min and aging treatment at 185 °C/13 h

Expression and Clinical Significance of ZO-1 in Patients with Non-small Cell Lung Cancer

Background and objective It has been proven that the expression of ZO-1 (zonula occluden-1) was correlated with the growth and metastasis of cancer. The aim of this study is to investigate the relationship between ZO-1 gene expression and clinicopathologic parameters and the survival rates in the patients with non-small cell lung cancer (NSCLC). Methods The expression levels of ZO-1 gene in 101 patients with NSCLC and lung tissues of 61 patients with benign lung disease were detected by real-time PCR, Western blot and immunohistochemical techniques. Results There was a significant statistic difference of ZO-1 mRNA expression levels between carcinoma group and control group (P < 0.01). In carcinoma group, adjacent group and control group, ZO-1 protein expression levels were with significantly statistic difference between each other (P < 0.01 or P < 0.05). There was a significantly statistic difference between ZO-1 mRNA expression levels in survival during 2 years follow-up group and death during 2 years follow-up group (t=-5.61, P < 0.01). Conclusion ZO-1 is a valuable predictive marker for NSCLC patients

Gain of UBE2D1 facilitates hepatocellular carcinoma progression and is associated with DNA damage caused by continuous IL-6

Abstract Background Hepatocellular carcinoma (HCC) is the most common type of liver cancer with increasing incidence and poor prognosis. Ubiquitination regulators are reported to play crucial roles in HCC carcinogenesis. UBE2D1, one of family member of E2 ubiquitin conjugating enzyme, mediates the ubiquitination and degradation of tumor suppressor protein p53. However, the expression and functional roles of UBE2D1 in HCC was unknown. Methods Immunohistochemistry (IHC), western blotting, and real-time PCR were used to detect the protein, transcription and genomic levels of UBE2D1 in HCC tissues with paired nontumor tissues, precancerous lesions and hepatitis liver tissues. Four HCC cell lines and two immortalized hepatic cell lines were used to evaluate the functional roles and underlying mechanisms of UBE2D1 in HCC initiation and progression in vitro and in vivo. The contributors to UBE2D1 genomic amplification were first evaluated by performing a correlation analysis between UBE2D1 genomic levels with clinical data of HCC patients, and then evaluated in HCC and hepatic cell lines. Results Expression of UBE2D1 was significantly increased in HCC tissues and precancerous lesions and was associated with reduced survival of HCC patients. Upregulation of UBE2D1 promoted HCC growth in vitro and in vivo by decreasing the p53 in ubiquitination-dependent pathway. High expression of UBE2D1 was attributed to the recurrent genomic copy number gain, which was associated with high serum IL-6 level of HCC patients. Further experiments showed that continuous IL-6 activated the DNA damage response and genomic instability by repressing DNA damage checkpoint protein RAD51B. Moreover, continuous IL-6 could significantly facilitate the HCC growth especially with the genomic gain of UBE2D1. Conclusions Our findings showed that UBE2D1 played a crucial role in HCC progression, and suggested a novel pattern of continuous IL-6 to promote cancers by inducing the genomic alterations of specific oncogenes

Influence of Density Gradient on the Compression of Functionally Graded BCC Lattice Structure

In this paper, five grading functional gradient lattice structures with a different density perpendicular to the loading direction were proposed, and the surface morphology, deformation behavior, and compression properties of the functional gradient lattice structures prepared by selective laser melting (SLM) with Ti-6Al-4V as the building material were investigated. The results show that the characteristics of the laser energy distribution of the SLM molding process make the spherical metal powder adhere to the surface of the lattice structure struts, resulting in the actual relative density of the lattice structure being higher than the designed theoretical relative density, but the maximum error does not exceed 3.33%. With the same relative density, all lattice structures with density gradients perpendicular to the loading direction have better mechanical properties than the uniform lattice structure, in particular, the elastic modulus of LF, the yield strength of LINEAR, and the first maximum compression strength of INDEX are 28.99%, 16.77%, and 14.46% higher than that of the UNIFORM. In addition, the energy absorption per unit volume of the INDEX and LINEAR is 38.38% and 48.29% higher, respectively, than that of the UNIFORM. Fracture morphology analysis shows that the fracture morphology of these lattice structures shows dimples and smooth planes, indicating that the lattice structure exhibits a mixed brittle and ductile failure mechanism under compressive loading. Finite element analysis results show that when the loading direction is perpendicular to the density gradient-forming direction, the higher density part of the lattice structure is the main bearing part, and the greater the density difference between the two ends of the lattice structure, the greater the elastic modulus

Electrical switching of perpendicular magnetization in L10 FePt single layer

Electrical manipulation of magnetization is essential for integration of magnetic functionalities such as magnetic memories and magnetic logic devices into electronic circuits. The current induced spin-orbit torque (SOT) in heavy metal/ferromagnet (HM/FM) bilayers via the spin Hall effect in the HM and/or the Rashba effect at the interfaces provides an efficient way to switch the magnetization. In the meantime, current induced SOT has also been used to switch the in-plane magnetization in single layers such as ferromagnetic semiconductor (Ga,Mn)As and antiferromagnetic metal CuMnAs with globally or locally broken inversion symmetry. Here we demonstrate the current induced perpendicular magnetization switching in L10 FePt single layer. The current induced spin-orbit effective fields in L10 FePt increase with the chemical ordering parameter (S). In 20 nm FePt films with high S, we observe a large charge-to-spin conversion efficiency and a switching current density as low as 7.0E6 A/cm2. We anticipate our findings may stimulate the exploration of the spin-orbit torques in bulk perpendicular magnetic anisotropic materials and the application of high-efficient perpendicular magnetization switching in single FM layer