Associations of house characteristics with indoor dampness and measured moisture: Results from three New Zealand House Condition Surveys in 2005, 2010 and 2015

No description supplie

Additional file 1: of Effects of treatment changes on asthma phenotype prevalence and airway neutrophil function

Methods S1. Details of the safety protocol for the sub-optimisation of treatment, skin-prick testing, sputum induction and processing, and sputum flow cytometry. Table S1. Number of participants in the change in treatment study using ICS and/or LABA. (DOCX 30 kb

Characteristics of study population.

<p>Characteristics of study population.</p

Neuropsychological test scores based on the bottom 5^th, 10^th and 20^th percentiles for comparison and collision repair workers.

<p>Neuropsychological test scores based on the bottom 5^th, 10^th and 20^th percentiles for comparison and collision repair workers.</p

Neuropsychological test scores for collision repair workers stratified by employment duration.

<p>Neuropsychological test scores for collision repair workers stratified by employment duration.</p

Metagenomic Detection of Viruses in Aerosol Samples from Workers in Animal Slaughterhouses

<div><p>Published studies have shown that workers in animal slaughterhouses are at a higher risk of lung cancers as compared to the general population. No specific causal agents have been identified, and exposures to several chemicals have been examined and found to be unrelated. Evidence suggests a biological aetiology as the risk is highest for workers who are exposed to live animals or to biological material containing animal faeces, urine or blood. To investigate possible biological exposures in animal slaughterhouses, we used a metagenomic approach to characterise the profile of organisms present within an aerosol sample. An assessment of aerosol exposures for individual workers was achieved by the collection of personal samples that represent the inhalable fraction of dust/bioaerosol in workplace air in both cattle and sheep slaughterhouses. Two sets of nine personal aerosol samples were pooled for the cattle processing and sheep processing areas respectively, with a total of 332,677,346 sequence reads and 250,144,492 sequence reads of 85 bp in length produced for each. Eukaryotic genome sequence was found in both sampling locations, and bovine, ovine and human sequences were common. Sequences from WU polyomavirus and human papillomavirus 120 were detected in the metagenomic dataset from the cattle processing area, and these sequences were confirmed as being present in the original personal aerosol samples. This study presents the first metagenomic description of personal aerosol exposure and this methodology could be applied to a variety of environments. Also, the detection of two candidate viruses warrants further investigation in the setting of occupational exposures in animal slaughterhouses.</p></div

Identity of virus contigs as reported by BLASTN search against the non-redundant genbank nucleotide database, and subsequent taxonomic assignment by MEGAN.

<p>Identity of virus contigs as reported by BLASTN search against the non-redundant genbank nucleotide database, and subsequent taxonomic assignment by MEGAN.</p

Summary of mapping statistics for WU polyomavirus and human papillomavirus 120 sequences identified in the cattle processing area aerosol sample, when mapped to reference genomes GU296398.1 for WUPyV, and JQ963485.1 for HPV120, using the Bowtie 2 software [28].

<p>Summary of mapping statistics for WU polyomavirus and human papillomavirus 120 sequences identified in the cattle processing area aerosol sample, when mapped to reference genomes GU296398.1 for WUPyV, and JQ963485.1 for HPV120, using the Bowtie 2 software <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072226#pone.0072226-Langmead1" target="_blank">[28]</a>.</p

Summary of the results reported in <b>Table 2</b>.

<p>The effect of face-to-face recruitment on follow-up participation compared to offline recruitment is shown. Given that the studies are highly heterogeneous by design and target different populations, the pooled estimate should be considered as an average of the study-specific effects rather than as a causal estimate.</p

Data summary by cohort and country.

<p>*Cohort size = Number of enrolled participants eligible for this study (see text for details regarding the inclusion criteria). Participants at follow up (%) = number of participants at follow-up divided by cohort size (x100).</p>1<p>Data referred to the percentage of Internet users in 2011 and were gathered from the International Telecommunication Union (ITU, <a href="http://www.itu.int" target="_blank">www.itu.int</a>), the United Nations specialized agency for information and communication technologies.</p><p>Data summary by cohort and country.</p