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Imunoenzimni test i lanÄana reakcija polimerazom uz prethodnu reverznu transkripciju za dokazivanje perzistentne zaraze virusom virusnog proljeva goveda u skupnom uzorku krvnog seruma - kratko priopÄenje.
The applicability and reliability were investigated of antigen enzyme linked immunosorbent assay (Ag ELISA) and reverse transcription polymerase chain reaction (RT-PCR) for the diagnosis of bovine viral diarrhoea (BVD) in pooled serum samples of persistently infected cattle. In this study 968 serum samples were tested and persistent infection was confirmed by Ag ELISA in six animals by sampling twice at a four week interval. Positive samples were tested undiluted and diluted by Ag ELISA and RT-PCR. Sera were diluted (fivefold) and tested once again by both methods. All undiluted samples were tested positive by Ag ELISA, while the end point dilution was 1:5. When RT-PCR was used, samples were shown to be positive to 1:125, and in one sample in a dilution of 1:625. We can conclude that Ag ELISA can be useful in the diagnosis of BVD in a maximum five pooled serum samples from persistently infected animals. RT-PCR shows greater sensitivity and thus is more appropriate for screening purposes when pooling of samples is recommended.Istražena je primjenjivost i pouzdanost Ag ELISA-e i lanÄane reakcije polimerazom uz prethodnu reverznu transkripciju (RT-PCR) u dijagnostici virusnoga proljeva u skupnom uzorku krvnog seruma perzistentno zaraženih goveda. Perzistentna zaraza dokazana je dvokratnim uzorkovanjem s razmakom od Äetiri tjedna. Ukupno je Ag ELISA-om bili pretraženo 968 uzoraka seruma goveda, a perzistentna je zaraza utvrÄena u Å”est goveda. Uzorci seruma svih Å”est goveda bili su peterostruko razrijeÄeni. NerazrijeÄeni i razrijeÄeni uzorci bili su pretraženi usporedno Ag ELISA-om i RT-PCR om. Svi nerazrijeÄeni uzorci dali su pozitivan rezultat s obje metode. RazrijeÄeni uzorci pretraživani Ag ELISA-om pokazali su pozitivan rezultat do razrijeÄenja 1:5. Svi razrijeÄeni uzorci pretraživani RT PCR-om bili su pozitivni do razrjeÄenja 1:125, a jedan uzorak do razrjeÄenja 1:625. ZakljuÄno se može reÄi da se Ag ELISA može rabiti u dijagnostici virusnoga proljeva goveda u skupnim uzorcima do najviÅ”e pet goveda. RT-PCR pokazao je veÄu osjetljivost i specifiÄnost u pretraživanju skupnih uzoraka krvnog seruma goveda