Effect of ethanolic extract of pod Prosopis farcta plant on neuronal density of anterior horn following sciatic nerve compression in Rats

Background and Objective: After axotomy or the compersion the nerve, the death of spinal cord nerves cell body occur. Compersion is one of the factors causing the degeneration of the spinal cord cell body. This degeneration is due to the reversed factors of the damaged area that have reached to cell body. Prosopis farcta is a member of leguminosae family and mimosaceae subfamily. The purpose of this study was to investigate the effect of ethanolic extract of pod prosopis farcta plant, on neuronal density of anterior horn following sciatic nerve compression in rat. Materials and Methods: This experimental study was performed on thirty male wistar rats with the age of about three months years and 300-350 gr weight. The animals were divided into five groups. A) control, B) compression, C) compression + treatment with 25 mg/kg ethanolic extract, D) compression + treatment with 50 mg/kg ethanolic extract and E: compression + treatment with 75 mg/kg ethanolic extract. After anesthetizing the rats, the muscle of thigh was splited and the sciatic nerve was kept under compersion, the muscle and skin were stitched subsequently. In the experimental groups the alchoholic extract of the prosopis farcta was injected to the rats with 25mg/kg, 50mg/kg, 75mg/kg dosage by the intrapritoneal way weekly. After 28 days of compresion, the rat, were put under the perfusion method and some samples were taken of their lumbar spinal cord and after tissue processes, 7 micron slide were provided of the samples serially. Slides were stained by toluidin blue, and some photos were taken and neuronal density of the alpha motoneurons alpha anterior horn of the spinal cord was calculated by the disector method. Data were analyzed using Minitab software, ANOVA and t- tests. Results: The neuronal density in the compression group (628±29.7) was decreased significantly in compare to the control group (1562±35.3) (P<0.05). The neuronal density in group C (1070±91), increased significantly in compare to the compression group (p<0.05). The neuronal density in group D (1117±62.8) and group E (1669±86.5) significantly increased in compare to the compression group (P<0.05). Conclusion: This study showed that alchoholic extract of the prosopis farcta has a neuroprotective effect following sciatic nerve compression in rats

Effect of aquatic extract of Cannabis sativa leaves on degeneration of alpha motoneurons in spinal cord after sciatic nerve compression in Rats

Background and Objective: Neurons are injured under physical, chemical and pathological conditions. The effects of injuries in peripheral nervous system returns as retrograde to the cell body of neurons in central nervous system and causes brain and spinal degeneration. This study was done to evaluate the effect of aquatic extract of Cannabis sativa leaves on degeneration of alpha motoneurons in spinal cord after sciatic nerve compression in Rats. Materials and Methods: This experimental study was carried out on thirty two male Wistar rats, weighing 300-350 grams. Animals were divided into four groups each consisting eight members A: control, B: compression, C: compression + treatment with 25 mg/kg aquatic extract, D: compression + treatment with 50 mg/kg aquatic extract. In order to induce compression in B, C and D, after cutting the right thigh muscle, Sciatic nerve of thigh was exposed to compression for 60 seconds using locker pincers. The first extract injection was done intraperitoneally immediately after compression and the second intera peritoneal injection was done 7 days later. 28 days after compression, the Lumbar spinal cord were dissected, fixed and stained with toluidine blue. The density of alpha motoneurons was measured using dissector and stereological methods. Data was analyzed with using Minitab-13 software, ANOVA and Tukey tests. Results: Neuronal density was 611.5±34.2 and 1633.4±30.7 in compression and control groups respectively (P<0.001). There was a meaningful statistical increase in neuronal density of group C (1278.6±28.1) in comparing compression group (P<0.001). The neuronal density in group (D) (1549.8±87.7), significantly increased in comparison with group (B) (P<0.001). Conclusion: This study showed that aquatic extract of Cannabis sativa leaves increases the density of alpha motoneurons in spinal cord after sciatic nerve compression in Rats. The increase in neuronal density is relevant to the amount of extract used