Slow GABAA mediated synaptic transmission in rat visual cortex

<p>Abstract</p> <p>Background</p> <p>Previous reports of inhibition in the neocortex suggest that inhibition is mediated predominantly through GABA_Areceptors exhibiting fast kinetics. Within the hippocampus, it has been shown that GABA_Aresponses can take the form of either fast or slow response kinetics. Our findings indicate, for the first time, that the neocortex displays synaptic responses with slow GABA_Areceptor mediated inhibitory postsynaptic currents (IPSCs). These IPSCs are kinetically and pharmacologically similar to responses found in the hippocampus, although the anatomical specificity of evoked responses is unique from hippocampus. Spontaneous slow GABA_AIPSCs were recorded from both pyramidal and inhibitory neurons in rat visual cortex.</p> <p>Results</p> <p>GABA_Aslow IPSCs were significantly different from fast responses with respect to rise times and decay time constants, but not amplitudes. Spontaneously occurring GABA_Aslow IPSCs were nearly 100 times less frequent than fast sIPSCs and both were completely abolished by the chloride channel blocker, picrotoxin. The GABA_Asubunit-specific antagonist, furosemide, depressed spontaneous and evoked GABA_Afast IPSCs, but not slow GABA_A-mediated IPSCs. Anatomical specificity was evident using minimal stimulation: IPSCs with slow kinetics were evoked predominantly through stimulation of layer 1/2 apical dendritic zones of layer 4 pyramidal neurons and across their basal dendrites, while GABA_Afast IPSCs were evoked through stimulation throughout the dendritic arborization. Many evoked IPSCs were also composed of a combination of fast and slow IPSC components.</p> <p>Conclusion</p> <p>GABA_Aslow IPSCs displayed durations that were approximately 4 fold longer than typical GABA_Afast IPSCs, but shorter than GABA_B-mediated inhibition. The anatomical and pharmacological specificity of evoked slow IPSCs suggests a unique origin of synaptic input. Incorporating GABA_Aslow IPSCs into computational models of cortical function will help improve our understanding of cortical information processing.</p

Pathogenesis, diagnosis and management of pneumorrhachis

Pneumorrhachis (PR), the presence of intraspinal air, is an exceptional but eminent radiographic finding, accompanied by different aetiologies and possible pathways of air entry into the spinal canal. By reviewing the literature and analysing a personal case of traumatic cervical PR after head injury, we present current data regarding the pathoanatomy, clinical and radiological presentation, diagnosis and differential diagnosis and treatment modalities of patients with PR and associated pathologies to highlight this uncommon phenomenon and outline aetiology-based guidelines for the practical management of PR. Air within the spinal canal can be divided into primary and secondary PR, descriptively classified into extra- or intradural PR and aetiologically subsumed into iatrogenic, traumatic and nontraumatic PR. Intraspinal air is usually found isolated not only in the cervical, thoracic and, less frequently, the lumbosacral regions but can also be located in the entire spinal canal. PR is almost exceptional associated with further air distributions in the body. The pathogenesis and aetiologies of PR are multifold and can be a diagnostic challenge. The diagnostic procedure should include spinal CT, the imaging tool of choice. PR has to be differentiated from free intraspinal gas collections and the coexistence of air and gas within the spinal canal has to be considered differential diagnostically. PR usually represents an asymptomatic epiphenomenon but can also be symptomatic by itself as well as by its underlying pathology. The latter, although often severe, might be concealed and has to be examined carefully to enable adequate patient treatment. The management of PR has to be individualized and frequently requires a multidisciplinary regime

Plant communities of trampled habitats in North Korea

A syntaxonomic study of trampled plant communities in North Korea is presented. Analytic and synthetic methods of the Braun-Blanquet approach together with a numericsyntaxonomical analysis (cluster analysis and principal coordinate analysis ordination) were employed. With the exception of the Bryo-Saginetum japonicae, all the associations, such as the Artemisio asiaticae-Plantaginetum asiaticae, Plantagini depressae-Polygonetum avicularis, Polygonoavicularis-Potentilletum costatae, Eragrostio multicaulis-Plantaginetum depressae, Euphorbio maculatae-Centipedetum minimae, Digitario ectiniformis-Eleusinetum indicae and Setario viridis-Chlorisetum virgatae, are described for the first time. Some communities also include a number ofsubassociations. Trampled communities are found along edges of paths and in cracks among paving stones. Soils vary from loamy to sandy and skeletal. A phenomenon of seasonality in expression of ruderal communities was observed in North Korea. It is assumed that this seasonality is controlled by pattern of precipitation showing distinct climatic seasons (e.g. pre-monsoon and postmonsoon periods). The ruderal vegetation seasonality is supposed to become more pronounced towards tropical regions. Several mesophilous or slightly hygrophilous European species occur frequently in North Korean trampled communitiesincluding Chenopodium glaucum, C. ficifolium and Potentilla supina. Their occurrence in trampled habitats is hypothesized as being related to high air humidity and associated wet climate

SHARPNESS AND UNIQUENESS OF THE PHYTOSOCIOLOGICAL CLASSES OF SLOVAKIA

This study extracts the diagnostic, constant and dominant species of Slovak vegetation types based on statistical analysis of phytosociological data stored in the national vegetation database. The affinities of vascular plant, bryophyte and lichen species to the major syntaxa (alliances and classes) were calculated using a statistically defined coefficient of fidelity. Additionally, the evaluation of vegetation units by the criteria of sharpness and uniqueness was created. These criteria allow us to identify well-delimited alliances and classes or to point out those, for which delimitation is problematic and which are more difficult to define by statistical principles. The syntaxonomical revision and delimitation of some units with low values of sharpness and uniqueness should be considered in the future

ALPINE HEATHS IN THE WESTERN CARPATHIANS - A NEW APPROACH TO THEIR CLASSIFICIATION

<img title="dubravcova_512" src="http://ojs.uniroma1.it//public/site/images/francesco_spada/dubravcova_512.jpg" alt="dubravcova_512" width="512" height="444" /

Design and application of a novel brain slice system that permits independent electrophysiological recordings from multiple slices

We describe a novel brain slice system ‘SliceMaster’ that allows electrophysiological recordings from eight brain slices independently. The system consists of two autonomous units each supporting four modular brain slice chambers enabling high signal-to-noise ratio recordings, each chamber has one stimulation electrode, one recording electrode, a twin camera system and a solution application system. The positioning of both electrodes and cameras are controlled from a remote user console. The software both acquires and performs on-line analysis of the data. We have demonstrated utility of this system in obtaining recordings of spontaneous firing activity and evoked synaptic activity from mouse hippocampal slices, with reduced variability within and between experiments. Furthermore, we show recordings of population spikes from the perirhinal cortex, indicating applicability of this system for further brain regions. In addition, stable recordings could be maintained until recording was terminated after 3 h, permitting investigation of the induction and maintenance of synaptic plasticity. Recordings of spontaneous and synaptic activity, and effects of pharmacological and electrophysiological manipulation, were consistent with reports using conventional methods. However, the described system permits concurrent and independent recordings from eight brain slices, thus improving throughput, statistical design, and reducing animal use