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Not AvailableSafflower (Carthamus tinctorius L.) is an important edible oilseed crop grown worldwide. Wilt caused by Fusarium oxysporurn f sp. carthami Klisiewicz and Houston is the major yield losing disease in safflower growing areas. It causes yield loss up to 93 % in susceptible varieties (Sastry and Ramchandram, 1994). The fungus persists in the soil and on plant debris, and is seed-borne in internal tissue of the hull and seed coat. Repeated planting of safflower in the same field enhances the fungus inoculum in the soil and the disease becomes serious. The wilting starts from the early seedling stage and continue up to the maturity of the crop. Screening the breeding material in wilt sick plot is crucial to identify resistant genotypes in resistance breeding programme. Standardized wilt inoculum load has to be maintained in wilt sick plot to ensure uniform disease incidence, otherwise, there would be chances of selecting false resistant types which would later hamper the resistance breeding programme. Also screening in sick plot takes about 120 days for recording of observations at different stages. To surmount these practical problems, attempts were made to identify molecular markers flanked to wilt resistant gene in the cultivateg species (c. tinctorius L.).Not Availabl

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Not AvailableThe elite Indian rice variety, Naveen is highly susceptible to major biotic and abiotic stresses such as blast, bacterial blight (BB), gall midge (GM) and drought which limit its productivity in rainfed areas. In the present study, a combined approach of marker-assisted forward (MAFB) and back cross (MABC) breeding was followed to introgress three major genes, viz., Pi9 for blast, Xa21 for bacterial blight (BB), and Gm8 for gall midge (GM) and three major QTLs, viz., qDTY1.1, qDTY2.2 and qDTY4.1 conferring increased yield under drought in the background of Naveen. At each stage of advancement, gene-based/linked markers were used for the foreground selection of biotic and abiotic stress tolerant genes/QTLs. Intensive phenotype-based selections were performed in the field for identification of lines with high level of resistance against blast, BB, GM and drought tolerance without yield penalty under non-stress situation. A set of 8 MAFB lines and 12 MABC lines with 3 to 6 genes/QTLs and possessing resistance/tolerance against biotic stresses and reproductive stage drought stress with better yield performance compared to Naveen were developed. Lines developed through combined MAFB and MABC performed better than lines developed only through MAFB. This study exemplifies the utility of the combined approach of marker-assisted forward and backcrosses breeding for targeted improvement of multiple biotic and abiotic stress resistance in the background of popular mega varieties.Not Availabl

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