Scatterplot showing 297 resting-associated vocalizations recorded from 15 captive Asian house shrews separated by the first two discriminant functions.

<p>The first discriminant function was mainly correlated with frequency-related acoustic parameters (<i>peak f0</i> falling behind the rest), and the second discriminant function was correlated with the acoustic parameters <i>peak f0</i> and <i>noisy percentage</i>.</p

Results of nested two-way ANOVA for effect of the factors sex and individuality on the acoustic parameters measured from resting-associated vocalizations of the studied Asian house shrews.

<p>Nested two-way ANOVA F-tests; ** <i>p</i><0.001.</p><p>Results of nested two-way ANOVA for effect of the factors sex and individuality on the acoustic parameters measured from resting-associated vocalizations of the studied Asian house shrews.</p

Representative resting-associated vocalizations emitted by captive Asian house shrews.

<p>Spectrogram (below) and waveform (above) of resting-associated vocalizations preceded by clearly visible non-vocal low clicks, with single low clicks (marked with arrows) simultaneously occurring in the recordings of one resting male (a) and three resting females (b), (c) and (d). Spectrogram settings: Hamming window, FFT length 512, frame size 50% and overlap 93.75%.</p

Descriptive statistics of acoustic parameters measured from randomly selected “tonal with low click” and “partially noisy” resting-associated vocalizations provided for males and females separately and for all studied individuals.

<p><i>N</i> =  number of individuals, <i>n</i> =  number of vocalizations.</p><p>Descriptive statistics of acoustic parameters measured from randomly selected “tonal with low click” and “partially noisy” resting-associated vocalizations provided for males and females separately and for all studied individuals.</p

Occurrence of resting-associated vocalization categories in Asian house shrews.

<p>Occurrence of the six defined categories in each of the 15 studied Asian house shrew individuals (a) and in males and females (b). M =  male, F =  female, <i>N</i> =  number of individuals, <i>n</i> =  number of vocalizations.</p

Summary and definitions of resting-associated vocalization categories found in the captive Asian house shrew.

<p>Summary and definitions of resting-associated vocalization categories found in the captive Asian house shrew.</p

Dataset S1 from Equilibrium dynamics of European pre-industrial populations: the evidence of carrying capacity in human agricultural societies

Excel file containing all data we used

Additional file 5: Figure S4. of Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae

Figure 3. Enhancing of endocytic trafficking depends on Sortin2 structural features. S. cerevisiae parental line was grown on YPD 1% DMSO (control) and YPD supplemented with 20 μM of different Sortin2-structural analogs. Afterwards cells were incubated with 24 μM FM4-64 for 30 min at 4°C. Then turned to 28°C to be imaged subsequently by confocal microscopy at different incubation times. Images of twenty-five min incubation are shown. Two images are representative of 20 cells. The experiment was performed more than 3 times. Scale bar represents 5 μm

Additional file 4: Figure S3. of Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae

sla1 is sensitive to three different CPY-secretion triggering compounds. Parental (WT) and sla1 strains were grown in YPD medium supplemented with the indicated concentrations of Sortin2, furan, Brefeldin A and Endosidin1. The control condition (0 μM Sortin2) contained 1% DMSO. The presence of CPY was analyzed on the growth medium by dot-blot using a CPY monoclonal antibody

Additional file 2: Figure S1. of Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae

Sortin2 short time treatment does not inhibit growth of Sortin2 resistant mutants. S. cerevisiae parental line (WT) and Sortin2 resistant mutants were grown on YPD supplemented with 1% DMSO (control) and YPD supplemented with 47 μM Sortin2 (Sortin2). Growth performance was evaluated by OD600 at different incubation times. The assay was repeated twice with experimental triplicates