BOX-PCR is an adequate tool for typing of clinical Pseudomonas aeruginosa isolates

In this study, the BOX-PCR fingerprinting technique was evaluated for the discrimination of clinical Pseudomonas aeruginosa isolates. All isolates were typeable and nearly half showed unique banding patterns. According to our results, BOX-PCR fingerprinting is applicable for typing of Pseudomonas aeruginosa isolates and can be considered a useful complementary tool for epidemiological studies of members of this genus. (Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 4, pp. 734–738

The activity of granulocytes in children with recurrent infections of respiratory tract

Acute upper respiratory tract infection (URTI) is a common disease in young children. URTI contributes to approximately 20% of mortality in children younger than 5 years of age. Infections of the upper respiratory tract are the most common cause of viral morbidity in children. They represent the most frequent problem in general pediatric practice and are responsible for more than one third of school absences. On the other hand respiratory tract infections through MALT system play a positive role in maturation and development of immune system. Many different immunologic disturbances can occur during the development of respiratory infections. Disorders of granulocyte functions are the principal cause of inflammation in the upper respiratory tract. Chemiluminescence of granulocytes is a renowned method for the estimation their overall activity as measured by the production of reactive oxygen species. The goal of the study was to evaluate chemiluminescent activity of granulocytes from 41 children with recurrent infections of the respiratory tract compared to 30 healthy children. Chemiluminescent response to all the examined stimuli in children with recurrent infections was significantly lower compared to control. In the examined group of 41 children depressed response to at least 1 stimulus was detected in 32 (78%) cases. The use of both fMLP and opsonised zymosan allowed to detect defect of granulocyte function in 93,75% cases. This defect may be related to delayed maturation of the immune system in certain children or may be induced by intensive therapy with antibiotics. Pneumonol. Alergol. Pol. 2005, 73, 160-166

Superior early diagnostic performance of a sensitive cardiac troponin assay as compared to a standard troponin test in the diagnosis of acute myocardial infarction

Background: New generation cardiac troponin assays have sufficient precision to detect and quantify plasma troponin concentrations below the lower threshold of detection of the currently employed troponin tests. However, diagnostic performance of the newer generation assays in daily clinical practice is not well established. Aim: To evaluate the diagnostic performance of a sensitive assay as compared to a standard assay in a single reading at admission in the diagnosis of acute myocardial infarction (AMI) in patients presenting to the Emergency Department with chest pain. Methods: The study comprised 187 consecutive patients admitted to the Institute of Cardiology in Warsaw in June and July 2010 with chest pain in whom the attending physician ordered troponin assay to rule AMI in or out. In all of these patients, in addition to the standard Dimension Flex Troponin I (Siemens Healthcare Diagnostics, Inc.) the sensitive Architect Stat Troponin I (Abbott Diagnostics) test was assayed. The triage of patients as well as all diagnostic and treatment decisions were left to the discretion of the attending physician who was blinded to the sensitive troponin test readings. The final diagnosis was adjudicated by a team of two cardiologists on the basis of all the available medical records except for sensitive troponin test results. Results: Mean age of the study cohort (n = 187) was 64.3 ± 13.9 years and 119 (63.6%) were males. The final diagnosis of AMI was adjudicated in 84 (44.9%) patients (mean age 67.5 ± 12.9 years; 119 [63.6%] males). Receiver operating characteristic (ROC) analysis showed greater area under the curve (AUC) for the sensitive cardiac troponin assay compared to the standard assay (AUC = 0.916, 95% CI = 0.866–0.951 vs AUC = 0.863, 95% CI = 0.806–0.909, respectively; p = 0.02) in a single reading at admission. Sensitive assay was characterised by higher sensitivity (87%), specificity (88%), positive (86%) and negative (89%) predictive values in the detection of AMI compared to the standard troponin test (82%, 81%, 78%, and 85% respectively). Conclusions: The newer generation sensitive cardiac troponin assay presented superior diagnostic accuracy in the diagnosis of AMI compared to the standard troponin test in a single reading at admission with improved sensitivity and specificity. The sensitive troponin assay has the potential to improve early detection and/or exclusion of AMI.Background: New generation cardiac troponin assays have sufficient precision to detect and quantify plasma troponin concentrations below the lower threshold of detection of the currently employed troponin tests. However, diagnostic performance of the newer generation assays in daily clinical practice is not well established. Aim: To evaluate the diagnostic performance of a sensitive assay as compared to a standard assay in a single reading at admission in the diagnosis of acute myocardial infarction (AMI) in patients presenting to the Emergency Department with chest pain. Methods: The study comprised 187 consecutive patients admitted to the Institute of Cardiology in Warsaw in June and July 2010 with chest pain in whom the attending physician ordered troponin assay to rule AMI in or out. In all of these patients, in addition to the standard Dimension Flex Troponin I (Siemens Healthcare Diagnostics, Inc.) the sensitive Architect Stat Troponin I (Abbott Diagnostics) test was assayed. The triage of patients as well as all diagnostic and treatment decisions were left to the discretion of the attending physician who was blinded to the sensitive troponin test readings. The final diagnosis was adjudicated by a team of two cardiologists on the basis of all the available medical records except for sensitive troponin test results. Results: Mean age of the study cohort (n = 187) was 64.3 +- 13.9 years and 119 (63.6%) were males. The final diagnosis of AMI was adjudicated in 84 (44.9%) patients (mean age 67.5 +- 12.9 years; 119 [63.6%] males). Receiver operating characteristic (ROC) analysis showed greater area under the curve (AUC) for the sensitive cardiac troponin assay compared to the standard assay (AUC = 0.916, 95% CI = 0.866–0.951 vs AUC = 0.863, 95% CI = 0.806–0.909, respectively; p = 0.02) in a single reading at admission. Sensitive assay was characterised by higher sensitivity (87%), specificity (88%), positive (86%) and negative (89%) predictive values in the detection of AMI compared to the standard troponin test (82%, 81%, 78%, and 85% respectively). Conclusions: The newer generation sensitive cardiac troponin assay presented superior diagnostic accuracy in the diagnosis of AMI compared to the standard troponin test in a single reading at admission with improved sensitivity and specificity. The sensitive troponin assay has the potential to improve early detection and/or exclusion of AMI

The Durability of an Organic–Inorganic Sol–Gel Interlayer in Al-GFRP-CFRP Laminates in a Saline Environment

The aim of the study was to assess the selected properties of a hybrid organic–inorganic silane sol–gel coating (HSG) used in hybrid fiber metal laminates (FML) in a corrosion environment. The HSG coating on the aluminum alloy was produced using 3M™ AC130-2 formulation consisting of 3-glycidoxypropyl-trimethoxysilane (GPTMS) and tetra-n-propoxyzirconium (zirconium(IV) propoxide) (TPOZ). Laminates consisted of aluminum alloy AA2024-T3 sheets, with carbon fiber reinforced polymers (CFRPs) and a glass fiber reinforced metal–composite structure (GFRP). Potentiodynamic and polarization curve and impedance (EIS) tests were carried out on HSG at ambient temperatures after 1 h and 150 h of soaking. Neutral 0.5 M NaCl and 0.8 M NaCl solutions were used for open circuit potential (OCP) and potentiodynamic tests, and 0.5 NaCl was used for the EIS test. A neutral salt spray (NSS) test was applied to laminates with a 12 week exposure period. The results obtained revealed that the HSG coating did not provide sufficient protection against corrosion of the aluminum alloy in direct contact with an aggressive environment but was effective as an interlayer. Local aluminum sheet perforation did not lead to delamination at the metal–composite interface regardless of the type or configuration of the composite. This confirms the durability of HSG used in FMLs

Psychosocial Effects of the Pandemic. Stress and Sense of Safety Experienced by Poles During the COVID-19 Pandemic in 2020–2021

The pandemic that broke out in 2019 had a significant impact on the lives of all social groups around the world. The imposed restrictions and mandatory quarantine were crucial to limit the virus’s spread. The research comprises an analysis of the psychosocial impact exerted by the pandemic that attempted to determine the response to the crisis caused by the COVID-19 pandemic and its aftermath. For this purpose, a study on social resilience in the pandemic era was worked out. The study consisted of several parts: stress and the sense of safety, education, trust and defined needs and the mass media in COVID-19. The research team decided to present the study’s results in a series of articles that will contribute to forming a complete picture of the community in the context of the analysed variables. The paper is the first in this series. It contains an analysis of variables intended to determine the level of the experienced sense of safety and its constituent, i.e., stress, and the identification of socio-demographic data strongly influencing the studied variables. The study comprised 559 individuals who were surveyed between May 2020 and November 2020 with the use of an online survey questionnaire. SPSS Statistics version 21.0 and PQStat were used to conduct statistical analyses and correlate and assess the correlation of responses. Also used were Chisquare, Fisher’s test and Pearson’s linear correlation coefficient. A logistic regression analysis was carried out for dichotomous variables. The results of the study indicate that the level of experienced stress is influenced by age, place of residence, gender and job security. The sense of safety is inversely correlated with stress, i.e., as stress increases, the sense of safety decreases, indicating a need to undertake appropriate measures to reduce stress. It may be interesting to compare the level of stress with, among other things, information retrieval from different sources. These results will be presented in the subsequent studies