Genetic diversity among some productive genotypes of tomato (Lycopersicon esculentum Mill.)

This research was done at Vegetable Experimental Farm, Faculty of Agriculture, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu during spring and summer seasons, 2007- 2009. D2-statistics revealed that β-carotene contributed maximally towards the genetic divergence followed by ascorbic acid, total soluble solids, alcohol insoluble solids, pericarp thickness, lycopene content and polygalacturonase activity. The 60 genotypes were grouped into 20 clusters. Fourteen (14) clusters were monogenotypic and cluster I possessed highest number of genotypes numbering 25. Out of 20 clusters, cluster VII is promising for minimum polygalacturonase activity and high average fruit weight, cluster VIII had highest number of locules per fruit, fruit yield per plant and yield per hectare and cluster XVII was superior for ascorbic acid. However, cluster XX was found promising for lycopene content, β-carotene and number of fruits per plants. The highest inter cluster D2 values were estimated between clusters XII and XX, followed by clusters XI and XX, clusters VII and XX, and clusters XV and XX, indicating that there is enough scope for the improvement of tomato crop by hybridization and selection.Key words: Genetic variability, genetic gain, heritability, tomato

Vocal cord palsy in an infant with myelomeningocoele

In this article we describe the case of a four-month-old male infant with myelomeningocoele, who presented with inspiratory stridor and vocal cord palsy (VCP). Hindbrain dysfunction is a leading cause of mortality and morbidity in children with neural tube defects. It is important to consider the above in the differential diagnosis of infants with breathing difficulties. A discussion of myelodysplasia, Arnold-Chiari malformations, bilateral VCP and anaesthesia management is presented.South Afr J Anaesth Analg, 2011;17(6):394-39

Impact of bias correction of regional climate model boundary conditions on the simulation of precipitation extremes

An accurate description of changes in extreme rainfall events requires high resolution simulations. Regional climate models (RCMs), where GCM data are used to provide input boundary conditions, are widely used as a way to resolve finer spatial scale phenomena. A problem with this, however, is that the inherent systematic biases within the GCM simulation are transferred to the RCM through the model boundaries. In this work we focus on the impact of bias correction of lateral and lower boundary conditions on simulated extreme rainfall events. Here three bias correction approaches are investigated. In increasing order of complexity, these are corrections for the mean, mean and variance, and the nested bias correction (NBC) approach that also corrects for lag-1 autocorrelations at nested timescales. These corrections are implemented on six-hourly GCM data taken from the GCM simulations which are used to drive the RCM along the RCM lateral boundaries. To evaluate the performance of bias correction on simulation of extreme rainfall events, daily precipitation extremes indices from the World Meteorological Organization (WMO) Expert Team on Climate Risk and Sectoral Climate Indicators (ET-CRSCI) are used. The results show that bias correction on the boundary conditions produce the results in significant improvement in extremes indices. It is clear that sea surface temperature (SST) plays an important role in driving the simulation. The results indicate that within the domain (far from boundaries) the errors in precipitation extremes are strongly dependent on the RCM, with a smaller effect coming from changes in the lateral boundary conditions

Study and Analysis of Exhaust Emission of Diesel Vehicles using Thermal IR Imagers

Exhaust emission analysis from diesel vehicles has received a lot of attention in recent times in the context of implementation of Bharat Stage-IV norms and thermal signature analysis for civil and military applications. The exhaust emission thermal IR signatures of military diesel vehicles such as truck and bus using a gas analyser and thermal imager under idling and accelerating conditions of these vehicles is investigated. Concentration and temperature of diesel exhaust emission CO, NOx, and HC remains almost constant during engine running in idle condition and varies with the engine acceleration. Exhaust gases maximum temperature reaches in the range of 240 °C - 270 °C during engine acceleration. A detailed investigation of thermal signature in mid wave infrared, 3 µm - 5 µm waveband and long wave infrared, 8 µm - 14 µm waveband is also presented under the same engine running conditions. Thermal image analysis exhibited that the area of thermal IR image of diesel vehicles truck and bus has been increased 0.077 per cent and 0.594 per cent, respectively with the engine acceleration. It has been observed that thermal signature of exhaust gases is a good tool for vehicle exhaust emission visualisation and analysis

Analysis of genetic diversity in female, male and half sibs willow genotypes through RAPD and SSR markers

Willows belong to the genus Salix (Salicaceae) and consist of large number of species with large phenotypic variations. As a result, it has a low diagnostic value for identifying pure species and interspecific hybrids. Genetic characterization of 34 reference genotypes (4 female, 10 male, and 20 half sibs) of Salix collected from Naganji Nursery of University of Horticulture and Forestry, Solan, Himachal Pradesh, India were analyzed using 10 SSRs and 15 RAPDs PCR-based molecular markers. RAPD analysis yielded 87 polymorphic fragments (98.9%), with an average of 5.8 polymorphic fragments per primer. Similarly, SSR analysis produced 33 bands, out of which 26 were polymorphic (78.8%) with an average of 2.6 polymorphic fragments per primer. The genetic diversity was high among the genotypes (Nei’s genetic diversity = 0.468 and Shannon’s information index = 0.659) as measured by combination of both RAPD and SSR markers. The mean coefficient of gene differentiation (Gst) was 0.034, indicating 96.6% of the genetic diversity resided within the genotypes. The genetic diversity among genotypes of Salix sp. was found to be high, suggesting the importance and feasibility of introducing elite genotypes from different origins for Salix germplasm conservation and breeding programs.Keywords: Salix sp., half sibs, molecular markers, genomic DNA fingerprinting.African Journal of Biotechnology Vol. 12(29), pp. 4578-458

The Genetics of Primary Haemorrhagic Stroke, Subarachnoid Haemorrhage and Ruptured Intracranial Aneurysms in Adults

Background: The genetic basis of haemorrhagic stroke has proved difficult to unravel, partly hampered by the small numbers of subjects in any single study. A meta-analysis of all candidate gene association studies of haemorrhagic stroke (including ruptured subarachnoid haemorrhage and amyloid angiopathy-related haemorrhage) was performed, allowing more reliable estimates of risk.Methods: A systematic review and meta-analysis of all genetic studies in haemorrhagic stroke was conducted. Electronic databases were searched until and including March 2007 for any candidate gene in haemorrhagic stroke. Odds ratio (OR) and 95% confidence intervals (CI) were determined for each gene disease association using fixed and random effect models.Results: Our meta-analyses included 6,359 cases and 13,805 controls derived from 55 case-control studies, which included 12 genes (13 polymorphisms). Statistically significant associations with haemorrhagic stroke were identified for those homozygous for the ACE/I allele (OR, 1.48; 95% CI, 1.20-1.83; p = 0.0003) and for the 5G allele in the SERPINE1 4G/5G polymorphism (OR, 1.42; 95% CI, 1.03-1.96; p = 0.03). In addition, both epsilon 2 and epsilon 4 alleles of APOE were significantly associated with lobar haemorrhage (OR, 1.81; 95% CI, 1.26-2.62; p = 0.002 and OR, 1.49; 95% 1.08-2.05; p = 0.01 respectively). Furthermore, a significant protective association against haemorrhagic stroke was found for the factor V Leiden mutation (OR, 0.30; 95% CI, 0.10-0.87; p = 0.03).Conclusion: Our data suggests a genetic contribution to some types of haemorrhagic stroke, with no overall responsible single gene but rather supporting a polygenic aetiology. However, the evidence base is smaller compared to ischaemic stroke. Importantly, for several alleles previously found to be associated with protection from ischaemic stroke, there was a trend towards an increased risk of haemorrhagic stroke

Voltage controlled terahertz transmission through GaN quantum wells

We report measurements of radiation transmission in the 0.220--0.325 THz frequency domain through GaN quantum wells grown on sapphire substrates at room and low temperatures. A significant enhancement of the transmitted beam intensity with the applied voltage on the devices under test is found. For a deeper understanding of the physical phenomena involved, these results are compared with a phenomenological theory of light transmission under electric bias relating the transmission enhancement to changes in the differential mobility of the two-dimensional electron gas

Linkage analysis for drought tolerance in kharif rice of Assam using microsatellite markers

371-375Drought stress in rainfed ecosystem significantly limits the production of Ranjit, the most predominant high yielding rice variety of Assam. A mapping population comprising 85 F4 individuals between Ranjit and a drought tolerant cultivar, ARC10372 was developed and genotyped with 80 microsatellite markers in order to understand the genetic basis of drought tolerance. The linkage map constructed based on a framework linkage map using these markers showed that the marker loci were distributed across 12 chromosomes spanning a distance of 273.4 cM with an average interval of 3.41 cM between marker loci. Most of the marker loci were found to be in good fit with the expected Mendelian segregation ratio; however, thirteen marker loci in total showed segregation distortion on six chromosomes. The linkage map generated in the study will facilitate mapping of quantitative trait loci imparting drought tolerance in rice of Assam and their map-based cloning

Case Study: Extreme Weight Making Causes Relative Energy Deficiency, Dehydration and Acute Kidney Injury in a Male Mixed Martial Arts Athlete.

The aim of the present case study was to quantify the physiological and metabolic impact of extreme weight cutting by an elite male MMA athlete. Throughout an 8-week period, we obtained regular assessments of body composition, resting metabolic rate (RMR), VO2peak and blood clinical chemistry to assess endocrine status, lipid profiles, hydration and kidney function. The athlete adhered to a "phased" weight loss plan consisting of 7 weeks of reduced energy (ranging from 1300 - 1900 kcal.d-1) intake (phase 1), 5 days of water loading with 8 L per day for 4 days followed by 250 ml on day 5 (phase 2), 20 h fasting and dehydration (phase 3) and 32 h of rehydration and refuelling prior to competition (phase 4). Body mass declined by 18.1 % (80.2 to 65.7 kg) corresponding to changes of 4.4, 2.8 and 7.3 kg in phase 1, 2 and 3, respectively. We observed clear indices of relative energy deficiency, as evidenced by reduced RMR (-331 kcal), inability to complete performance tests, alterations to endocrine hormones (testosterone: 6 mmol.L-1). Moreover, severe dehydration (reducing body mass by 9.3%) in the final 24 hours prior to weigh-in induced hypernatremia (plasma sodium: 148 mmol.L-1) and acute kidney injury (serum creatinine: 177 μmol.L-1). These data therefore support publicised reports of the harmful (and potentially fatal) effects of extreme weight cutting in MMA athletes and represent a call for action to governing bodies to safeguard the welfare of MMA athletes