MOESM4 of Male size, not female preferences influence female reproductive success in a poeciliid fish (Poecilia latipinna): a combined behavioural/genetic approach

Additional file 4. Results paternity analysis and offspring life history data

Allele specific expression (ASE) of <i>androgen receptor alpha</i> in <i>Poecilia formosa</i>.

<p>Percentages of the expression of the <i>P</i>. <i>mexicana</i>-derived allele in three tissues (ovary, brain, and gill) of <i>P</i>. <i>formosa</i> are presented. 0.5 indicates an equal (i.e., 1:1) expression of both alleles.Statistical significance was evaluated with an Analysis of Variance (ANOVA) and pairwise post-hoc-tests (** P< 0.001).</p

Structural comparison of <i>P</i>. <i>formosa</i> Ars with those of other species.

<p>The numbers within each box indicate the percent amino acid identity of each putative domain (NTD: N-terminal domain, DBD: DNA binding domain (blue color), LBD: ligand binding domain (green color)), relative to <i>P</i>. <i>mexicana</i> Arα. The numbers above each box indicate the amino acids position of each domain. <i>P</i>. <i>formosa</i> had two alleles of both Arα and Arβ, pointing to its hybrid origin.</p

Gene expression profiling.

<p>This profiling presents each single gene expression distribution through all 3 tissues (ovary, gill, brain) among <i>P</i>. <i>formosa</i> and its ancestors. Expression levels are normalized relative to <i>P</i>. <i>mexicana</i> ovary expression data of 6 different pathway genes, <i>arα</i> (A), <i>arβ</i> (B), <i>cyp19a1</i> (C), <i>cyp19a2</i> (D), <i>erα</i> (E), <i>erβ1</i> (F). They are shown on a logarithmic scale. Red stars indicate that expression has not been detected in our study. * 0.01≤P≤0.05; ** P< 0.01.</p

Appendix A. General linear models (GLMs) on female life-history traits and additional matrotrophy analyses.

General linear models (GLMs) on female life-history traits and additional matrotrophy analyses

Appendix B. Supplementary information on the discriminant function analyses (DFAs) on female life-history traits.

Supplementary information on the discriminant function analyses (DFAs) on female life-history traits

The transformed fluorescence ratio K’ and associated coefficients of variation (CV, %).

<p>The transformed fluorescence ratio K’ and associated coefficients of variation (CV, %).</p

Prediction of impact of non-synonymous inferred amino acid substitutions (cf. Fig 2).

<p>Prediction of impact of non-synonymous inferred amino acid substitutions (cf. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0156209#pone.0156209.g002" target="_blank">Fig 2</a>).</p

MOESM1 of Male size, not female preferences influence female reproductive success in a poeciliid fish (Poecilia latipinna): a combined behavioural/genetic approach

Additional file 1. Binary choice test. Detailed description for the determination of female preference

The probes and the investigated heterozygous site.

<p>The red rectangle indicates the VIC-labeled MGB probe for the <i>P</i>. <i>mexicana</i>-derived allele. The blue rectangle indicates the FAM-labeled MGB probe for the <i>P</i>. <i>latipinna</i>-derived allele. The blue star represents the heterozygous marker site. Abbreviation in figure, Me: <i>P</i>. <i>mexicana</i>-derived allele, La: <i>P</i>. <i>latipinna</i>-derived allele.</p