RAPD markers assisted varietal identification and genetic purity test in pepper, Capsicum annuum

WOS: 000181210000003The potential of randomly amplified polymorphic DNA (RAPD) markers in varietal identification and genetic purity test of hybrid varieties was evaluated. Five Jalepeno hybrid varieties and their corresponding parents were screened for polymorphic RAPD markers with 12 arbitrary 10-mer primers. Among a total of 177 bands observed, 14 bands contributed by nine primers were polymorphic in the five pepper varieties. Furthermore, four RAM markers were found to be cultivar-specific markers for three hybrid varieties. Out of the 12 primers, six primers generated 11 useful RAPD markers to determine seed purity of all tested hybrid varieties. In conclusion, RAPD markers maybe useful for cultivar identification and hybrid purity test in pepper, Capsicum annuum, especially for routine seed quality control programme. (C) 2002 Elsevier Science B.V. All rights reserved

Can vigour tests estimate the storage potential of white-seeded beans?

This study was conducted to determine storage potential of white-seeded beans using the electrical conductivity and accelerated ageing tests recommended by ISTA for legumes. For this purpose, eight seed lots of flve white-seeded cultivars from research institutes and seed companies were stored at 5°and 25°C up to 10 months. Seed quality was determined before and during storage using standard germination, electrical conductivity and accelerated ageing tests. There was a greater reduction in both the standard and accelerated ageing germinations when seeds were stored at 25° than at 5°C. The electrical conductivity of seed lots increased during the storage period at both the temperatures. The electrical conductivity and accelerated ageing before storage were significantly correlated with standard germination after 10 months storage at 5°C (r=-0.814; r=0.796, P<0.05) and 25°C (r=-0.845; r=0.761, Plt;0.01; P<0.05), respectively. Therefore, it can be stated that the electrical conductivity and accelerated ageing tests can be used for storage management

The potential of vigour tests to identify differences in the extent of ageing in onion seeds

WOS: 000242545900017Twenty-eight seed lots of two onion cultivars (Diana F1 and Aki) were created by naturally aging seeds during storage at 10% MC and 5 degrees and 25 degrees C for up to 22 months to investigate the ability of the vigour tests to identify differences in the extent of seed aging and possibly vigour. All seed samples were subjected to the following tests: standard germination test, cool germination test, electrical conductivity (E.C.), sugar and amino acid leakage from seed. Germination declined after more prolonged ageing in Diana, but remained high in Aki. E.C., sugar and amino acid contents in the seed leachate increased in both cultivars even when germination remained high, indicating a decline in seed vigour. The E.C. and sugar leakage from seed samples were highly correlated with performance in the stress conditions of the. cool germination test (r=-0.911; r=-0.808, respectively). Leakage tests therefore appear to be suitable for evaluating seed quality in onion seeds and could be further developed as a rapid vigour test

Determination of resistant local varieties to Fusarium wilt (Fusarium oxysporum f.sp melonis) with molecular markers

10th EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae -- OCT 15-18, 2012 -- Antalya, TURKEYWOS: 000345687100069Fusarium wilt, caused by Fusarium oxysporum f. sp. melonis (F.o.m), is a soil-borne disease of melon (Cucumis melo L.). Fusarium oxysporum f. sp. melonis (F.o.m) causes serious economic losses in melon. Two dominant resistance genes have been identified, Fom-1 and Fom-2, which provide resistance to races 0 and 2, and races 0 and 1, respectively. The most effective control measure is the use of resistant varieties. We mainly tested 44 local melon local varieties from Egean region and different parts of Turkey to determine their resistance to Fusarium wilt with artifical inoculation (Races FOM 0, FOM 1, FOM 2, FOM 1,2 pathogen isolates inoculated to melon seedlings roots) and for Fom1 and Fom2 genes using SCAR (Sequence Characterized Amplified Region) and CAPS (Cleaved Amplified Polymorphic Sequences) molecular markers. Results of artifical inoculation tests and molecular markers tests for race FOM 2 were not consistent. According to morphological and molecular results, we were able to detect some resistant varieties for race FOM 0 and FOM 1. These varieties are: TR-40559 (Malatya, var. cantalupensis), TR-70699 (Manisa, var. inodorus), TR-61613 (Mugla, var. inodorus) and TR-69891 (Kirkagac, var. inodorus). It can be stated that these varieties are usable in future fusarium wilt resistant breeding programs.European Assoc Res Plant Breeding, Cukurova Univ, Minis Food, Agr & Livestock, Turkish Sci & Technol Council, Antalya Tarim, Manier Seed, Yuksel Seed, Syngenta, AG Seed, Fito Seed, Multi Seed, Nunhems, Rijk Zwaan, Bati Akdeniz Agr Res Inst, Alata Hort Res Sta