Research of Catholyte Water on Experimental Graffi Tumor on Hamsters. Kangen Device for Catholyte Water

The paper describes the results of a pilot study aimed at the investigation of the influence of catholyte (electrolyzed alkaline water) on the development of tumors. In the experiments solid Graffi tumor was transplanted subcutaneously in the back of the experimental group of Golden Syrian hamsters. Tumor appearance and growth were registered every day. Blood parameters were measured on the 10th and 25th day after transplantation and blood smears were prepared. Hamsters treated with catholyte developed tumor with some delay compared to untreated (drinking tap water) ones. Also, the tumor growth was slow and the survival rate was increased. The analysis of blood parameters and cell morphology has shown significant differences in the value of some hematometric parameters and morphological changes of some blood cells. The obtained results suggest about the beneficial influence of catholyte and the possible use of it as a supporting non-invasive therapy of cancer diseases.The studies of the author show that the highest quality of Catholyte water if from device Kangen. The research of  effect on Experimental Graffi Tumor on Hamsters is with co-authors  Reneta Toshkova,  Elissaveta Zvetkova and Georgi Gluhchev. Keywords: Graffi tumor, catholyte, tumor growth, survival rate, mortality, blood parameters. DOI: 10.7176/JMPB/59-01 Publication date:September 30th 201

2021 – 25 Years Scientific Research Center of Medical Biophysics

The Scientific Research Center of Medical Biophysics (SRCMB) carries out studies in the following directions – structure of water, origination of life and living matter, mountain water and longevity, high frequency color coronal discharge, nanotechnologies, astrobiology, biological effects in heavy water, entropy and time in living matter, visual analyzer, biophysical fields, biotechnologies, shungite.Scientific Board of SRCMB – Prof. Dr. Ignat Ignatov DSc (Director), Prof. Christos Drossinakis (Honorable Director), Lieselotte Eder (Deputy Director), Alexander Ignatov (manager), Dr. Igor Akszjonovics MD (Deputy Director), Prof. Stanislav Zenin, Dipl. Eng. Enrico Bauer, Dipl. Eng. Chavdar (Charlie) Stoyanov, Paul N. Kleindienst, Heide Trautwein (Deputy Director), Teodora Todorova (web designer), Tanya Metodieva, Polya Ivanova, Daniya Nachkova,  Verka Nikolova (Master in Mathematics), Svetla Damyanova (Master in Pharmacy), Gergana Genova, Maria Abazova (Bachelor in Medicine), Dipl. Eng. Erwin Lehki, Dipl. Eng. Tsolo Petkov, Dipl. Eng. Hristo and Silvia P. Deneva. Keywords: longevity, mountain water, origination of life and living matter, high frequency color coronal discharge, nanotechnologies DOI: 10.7176/JMPB/70-04 Publication date: April 30th 202

VITA Intense – Proofs for Anti-inflammatory, Antioxidant and Inhibition Growth of Tumor Cells Effects. Relaxing Effect of Nervous System. Anti Aging Influence

We studied the mathematical model of interaction with water VITA Intense of LavaVitae company (Austria). In this report are submitted data about the interaction of VITA Intense with water, obtained by non-equilibrium (NES) and differential-equilibrium energy spectrum (DNES) of water. The average energy (∆EH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of VITA Intense with water measured by NES- and DNES-methods is ∆E=-0.0136±0.0011 eV for VITA Intense. This result suggests the restructuring of ∆EH... O values among H2O molecules with a statistically reliable increase of local extremums in DNES-spectra. The research is performed for VITA Intense, with study of pH and oxidative reduction potential (ORP).  There is review of the effects of the chemical composition of VITA Intense – anti-inflammatory, antioxidant etc. With methods NES we show the following effects – relaxing effect of nervous system, anti-inflammatory and inhabitation of tumor cells. As results of these effects VITA Intense has anti aging influence. The base of this influence is anti-inflammatory effect.  This article deals with the review of the basic biophysical-biochemical and biological processes underlying the VITA Intense by LavaVitae company. The authors are studying their physical-chemical properties and biophysical and biological effects on human organism. Additionally, by using IR, NES, and DNES methods were investigated various samples of water from Bulgarian water springs: the melt water from Glacier Rosenlaui, Swiss Alps, as well as the human blood serum of people with excellent health and cancer patients between 50 and 70 years old. Other experiments were performed on a 1% (v/v) solution of VITA Intense in deionized water. As an estimation factor in NES and DNES was measured the values ​​of the average energy of hydrogen bonds (∆EH...O) among H2O molecules in water samples, as well as a local extremums  in the NES and DNES-spectra of various samples of water and the human blood serum at E = -0.1387 eV and λ = 8.95 μm. For a group of people in critical condition of life and patients with malignant tumors the greatest values ​​of local extremums in IR-, DNES-spectra were shifted to lower energies relative to the control healthy group. Further we applied this method for calculation of percent distribution of H2O molecules in all studied water samples according to energies of hydrogen bonds ranged from (-0.08 to -0.1387 eV). It was shown that mountain water is among the most important factors for human longevity and human health. The variety of ions (K+, Na+, Ca2+, Mg2+, Mn2+, Fe2+, Fe3+, Zn2+, SO42-, Cl-, HCO3-, CO32-), the chemical-physical parameters (pH, electroconductivity) and the decreased content of deuterium in studied water samples renders beneficial effects of these types of water on human health. We are applying the conclusions for the effects of mountain water on human health and longevity as base for the conclusion on the effects of VITA Intense. The base is also the results with methods NES and DNES on human serum and expected effects from VITA Intense. Keywords: VITA Intense, anti-inflammatory, antioxidant, anti-aging, mathematical model, NES, DNES

Electromagnetic Conception for the Eyesight in Additive Mixing of Colors. Rhodopsin and Bacteriorhodopsin. Еmotional Perception of Colors

This review article views predominately the structure and function of animal and bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) and their aspects of nano- and biotechnological usage. On an example of bacteriorhodopsin is described the method of its isolation from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, the solubilization with 0,5 % (w/v) SDS-Na and subsequent fractionation by methanol and gel filtration chromatography on Sephadex G-200 Column balanced with 0.09 M Tris-borate buffer (pH = 8,35) with 0,1 % (w/v) SDS-Na and 2,5 mM EDTA. Within the framework of the research the mechanism of color perception by the visual analyzer having the ability to analyze certain ranges of the optical spectrum, as colors was studied along with an analysis of the additive mixing of two colors. It was shown that at the mixing of electromagnetic waves with different wavelengths, the visual analyzer perceive them as separate or average wave length corresponding to mix color. In the report there is information about emotional perception of colors with new achievements. Keywords: vision, rhodopsin, iodopsin, bacteriorhodopsin, additive color mixing, emotional perceptio

Antibacterial and Antiviral Effects of Water Catholyte, Oxidal with Methylene Blue and Colloidal Silver

Coronavirus COVID-19 is an RNA virus with size 120 nm. It began spreading at the end of 2019 and at the beginning of 2020 in China, and it leads to a severe infectious disease 2019-nCoV. It resembles severe acute respiratory syndrome-related coronavirus (SARSr-CoV), and the new virus is labelled as SARS-CoV-2. Its genome is single-chained with positive strand. It contains a nucleotide with spiral symmetry. The coronavirus SARSr-CoV gets replicated via copying of the genetic material using the enzyme RNA-dependent RNA polymerase.The author considers a complex method of influence over SARS-CoV that includes intake of alkaline water Catholyte with negative oxidation reduction potential (ORP), food supplement Oxidal with Methylene blue and Colloidal Silver. Keywords: SARSr-CoV, water Catholyte, Oxidal with Methylene blue and Colloidal Silve

New Properties and Bio effects of High Quality Bulgarian Zeolite Rhodosorb-H®. and Cosmetic Water Rhodosorb-W®

The author studied the mathematical model of interaction with water of natural mineral and microporous crystalline mineral zeolite Rhodosorb-H from Bulgaria. Clinoptilolite is from the groups of minerals with name zeolite. Clinoptilolite is a natural zeolite comprising a microporous arrangement of silica and alumina tetrahedra. It has the complex formula: (Na,K,Ca)2-3Al3(Al,Si)2Si13O36·12H2O. The Bulgarian zeolite consists 80% clinoptilolite. In this report are submitted data about the interaction of zeolite Rhodosorb-H with water, obtained by non-equilibrium (NES) and differential-equilibrium energy spectrum (DNES) of water. The average energy (∆EH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of zeolite Rhodosorb-H with water measured by NES- and DNES-methods is ∆E=-0.0077±0.0011 eV for zeolite Rhodosorb-H. This result suggests the restructuring of ∆EH... O values among H2O molecules with a statistically reliable increase of local extremums in DNES-spectra. The research is performed for zeolite Rhodosorb-H with study of pH and oxidative reduction potential (ORP). The author studied the properties of Bulgarian cosmetic water Rhodosorb-W according the cosmetic water as control sample from the market. The research is with methods NES and DNES. The average energy (∆EH... O) of hydrogen Н…O-bonds among individual molecules H2O of cosmetic water Rhodosorb-W measured by NES- and DNES-methods is ∆E=-0.003±0.0011 eV. There is review of the effects of the chemical composition of Rhodosorb-W – anti-inflammatory (Climent, Corma, Iborra; 2005), antioxidant (Montinaro et al.;2013) etc. With methods NES we show the following effects – anti-inflammatory and inhibition of development as size and number of tumor cells, anti toxin. As results of these effects Rhodosorb-W has anti aging influence. The base of this influence is anti-inflammatory effect. This article deals with the review of the basic biophysical-biochemical and biological processes underlying the Rhodosorb-W . The author is studying their physical-chemical properties and biophysical and biological effects on human organism. Additionally, by using IR, NES, and DNES methods are investigated various samples of water from Bulgarian water springs: the melt water from Glacier Rosenlaui, Swiss Alps, as well as the human blood serum of people with excellent health and cancer patients between 50 and 70 years old (Ignatov, Mosin; 2012). Other experiments were performed on a 1% (v/v) solution of Rhodosorb-W in deionized water. As an estimation factor in NES and DNES was measured the values of the average energy of hydrogen bonds (∆EH...O) among H2O molecules in water samples, as well as a local extremums in the NES and DNES-spectra of various samples of water and the human blood serum at E = -0.1387 eV and λ = 8.95 μm. For a group of people in critical condition of life and patients with malignant tumors the greatest values of local extremums in IR-, DNES-spectra were shifted to lower energies relative to the control healthy group. Further we applied this method for calculation of percent distribution of H2O molecules in all studied water samples according to energies of hydrogen bonds ranged from (-0.08 to -0.1387 eV). Rhodosorb-W makes increasing of local extremum at E = -0.1387 eV and λ = 8.95 μm as indicator for re structuring of the hydrogen bond toward highest energy level at E = -0.1387 eV and λ = 8.95 μm. Zeolite Rhodosorb-H and cosmetic water Rhodosorb-W are with registered trade marks ®. Keywords: zeolite Rhodosorb-H, cosmetic water Rhodosorb-W, mathematical model, NES, DNES

Product of LavaVitae BOOST is Increasing of Energy of Hydrogen Bonds among Water Molecules in Human Body

We studied the mathematical model of interaction with water of BOOST of LavaVitae company (Austria). In this report are submitted data about the interaction of BOOST with water, obtained by non-equilibrium (NES) and differential-equilibrium energy spectrum (DNES) of water. The average energy (∆EH... O) of hydrogen Н…O-bonds among individual molecules H2O after treatment of BOOST with water measured by NES- and DNES-methods is ∆E=-0.0114±0.0011 eV. This result suggests the restructuring of ∆EH... O values among H2O molecules with a statistically reliable increase of local extremums in DNES-spectra. The research is performed for BOOST, with study of pH and oxidative reduction potential (ORP).  There is review of the effects of the chemical composition of BOOST – anti-inflammatory, energetic, stimulating etc. With methods NES we show the following effects – improvement of nervous conductivity and anti-inflammatory effect. This article deals with the review of the basic biophysical-biochemical and biological processes underlying the BOOST by LavaVitae company. The authors are studying their physical-chemical properties and biophysical and biological effects on human organism. Keywords: BOOST, anti-inflammatory, improvement conductivity of nervous system, mathematical model, NES, DNES

Research of Bioinfluence of Risimanski with Model Systems in Biophysics and Biochemistry as Base of Medical Effects

This paper presents the results of evaluation of possible biophysical methods and approaches for registering of various non-ionizing radiation (NIR) wave types of the human body in the electromagnetic and optical range. Many types of NIR (electromagnetic waves, infrared radiation, thermo radiation, bioluminescence) emitted from the human body were reviewed. In particular the results on of spontaneous biophoton emission and delayed luminescence from the human body are submitted along with infrared thermography (IRT) results. It was shown that 1 cm2 of skin generally emits ~85 photones for 1s. The intensity of biophoton emission ranges from 10−19 to 10−16 W/cm2 (approx. ~1–1000 photons.cm-2.s-1). The specific photon emission from part of the human thumb was detected as a spectrum of various colours with the method of Colour coronal spectral analysis on a device with an electrode made ​​of polyethylene terephthalate (PET hostafan) with applied electric voltage 15 kV, electric impulse duration 10 ms, and electric current frequency 15 kHz. It was established that photons corresponding to a red color emission of visible electromagnetic spectrum have energy at 1.82 еV. The orange color of visible electromagnetic spectrum has energy at 2.05, yellow – 2.14, blue-green (cyan) – 2.43, blue – 2.64, and violet – 3.03 eV. The reliable result measurement norm was at E ≥ 2.53 eV, while the spectral range of the emission was within 380–495 nm and 570–750 nm±5 nm. Also were estimated some important physical characteristics (energy of hydrogen bonds, wetting angle, surface tension) of water by the methods of non-equilibrium energy (NES) and differential non-equilibrium energy (DNES) spectrum of water, that helps understand in general how electromagnetic radiation interacts with water and establish the structural characteristics of water. Keywords: electromagnetic waves, infrared radiation, thermo radiation, bioluminescence, color coronal spectral analysis, NES, DNE

Rhodopsin and Bacteriorhodopsin. Electromagnetic Conception for the Eyesight in Additive Mixing and Еmotional Perception of Colors

This review article views predominately the structure and function of animal and bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) and their aspects of nano- and biotechnological usage. On an example of bacteriorhodopsin is described the method of its isolation from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, the solubilization with 0,5 % (w/v) SDS-Na and subsequent fractionation by methanol and gel filtration chromatography on Sephadex G-200 Column balanced with 0.09 M Tris-borate buffer (pH = 8,35) with 0,1 % (w/v) SDS-Na and 2,5 mM EDTA. Within the framework of the research the mechanism of color perception by the visual analyzer having the ability to analyze certain ranges of the optical spectrum, as colors was studied along with an analysis of the additive mixing of two colors. It was shown that at the mixing of electromagnetic waves with different wavelengths, the visual analyzer perceive them as separate or average wave length corresponding to mix color. In the report there is information about emotional perception of colors with new achievements. Keywords: vision, rhodopsin, iodopsin, bacteriorhodopsin, additive color mixing, emotional perception

Antiviral Effects of Water Catholyte, Oxidal with Methylene Blue and Colloidal Silver. Possible Mechanism of Influence over Coronavirus SARSr-CoV and SARSr-CoV-2 with Disease COVID-19

Coronavirus COVID-19 is an RNA virus with size 120 nm. It began spreading at the end of 2019 and at the beginning of 2020 in China, and it leads to a severe infectious disease 2019-nCoV. It resembles severe acute respiratory syndrome-related coronavirus (SARSr-CoV), and the new virus is labelled as SARS-CoV-2. Its genome is single-chained with positive strand. It contains a nucleotide with spiral symmetry. The coronavirus SARSr-CoV gets replicated via copying of the genetic material using the enzyme RNA-dependent RNA polymerase.The author considers a complex method of influence over SARS-CoV that includes intake of alkaline water Catholyte with negative oxidation reduction potential (ORP), food supplement Oxidal with Methylene blue and Colloidal Silver. Keywords: SARSr-CoV, water Catholyte, Oxidal with Methylene blue and Colloidal Silver DOI: 10.7176/JHMN/73-11 Publication date: April 30th 202