Research on the bio-distribution of interferon gamma-containing pharmacocytes

Viral hepatitis B and C- is a widespread infectious disease of the liver and is a potential threat to human life. Treatment of HCV and HBV requires prolonged (parenteral or oral) administration of antiviral and immune-stimulating agents, which often have serious side effects that lead to abrupt termination of the therapy and the development of viral resistance. Targeted delivery of drugs directly to the affected organ is one of the most promising areas that seek to improve the outcome of treatment of patients with chronic diseases. Among the various drug delivery systems, erythrocytic pharmacocytes are of special interest. They are a shade of red blood cells, that could be loaded with antiviral drugs and drugs that boost the immune system. Owing to the fact that erythrocytes are destroyed by mononuclear phagocytes in the liver, it is assumable that the EP serve as a promising and direct means of delivering antiviral drugs and immune-modulators into the liver parenchyma to treat viral hepatitis

Research on the bio-distribution of interferon gamma-containing pharmacocytes

Viral hepatitis B and C- is a widespread infectious disease of the liver and is a potential threat to human life. Treatment of HCV and HBV requires prolonged (parenteral or oral) administration of antiviral and immune-stimulating agents, which often have serious side effects that lead to abrupt termination of the therapy and the development of viral resistance. Targeted delivery of drugs directly to the affected organ is one of the most promising areas that seek to improve the outcome of treatment of patients with chronic diseases. Among the various drug delivery systems, erythrocytic pharmacocytes are of special interest. They are a shade of red blood cells, that could be loaded with antiviral drugs and drugs that boost the immune system. Owing to the fact that erythrocytes are destroyed by mononuclear phagocytes in the liver, it is assumable that the EP serve as a promising and direct means of delivering antiviral drugs and immune-modulators into the liver parenchyma to treat viral hepatitis

Establishment of small cell lung cancer cell lines and validation of their growth characteristics

Rapidly metastasizing lung cancer is the top killer in the United States and many other countries. In 2014, there were nearly 224 210 new cases of lung cancer and 159 260 predicted mortality from the disease in the US and approximately 44 488 new registered cases of lung cancer in 2012 with 80% mortality and 5 % survival rate within 10 years of diagnosis in the UK. Lung cancer is the most prevalent type of cancer in Kazakhstan accounting for nearly 22.1 % of all cancer cases. Small cell lung cancers (SCLCs) derived from the hormonal cells of the lung and classified as one of the most dedifferentiated cancers representing 10 - 15% of all lung cancers however showing extremely aggressive and rapid dissemination into various parts of the body. In this work we established SCLC cell line and characterised their growth for upcoming research purposes

Establishment of small cell lung cancer cell lines and validation of their growth characteristics

Rapidly metastasizing lung cancer is the top killer in the United States and many other countries. In 2014, there were nearly 224 210 new cases of lung cancer and 159 260 predicted mortality from the disease in the US and approximately 44 488 new registered cases of lung cancer in 2012 with 80% mortality and 5 % survival rate within 10 years of diagnosis in the UK. Lung cancer is the most prevalent type of cancer in Kazakhstan accounting for nearly 22.1 % of all cancer cases. Small cell lung cancers (SCLCs) derived from the hormonal cells of the lung and classified as one of the most dedifferentiated cancers representing 10 - 15% of all lung cancers however showing extremely aggressive and rapid dissemination into various parts of the body. In this work we established SCLC cell line and characterised their growth for upcoming research purposes

Restoration of mesenchymal stem cell function using pharmacological agents

The extensive characteristics of mesenchymal stem cells (MSCs), especially their capacity to replicate and differentiate into any type of fully developed cell make these cells suitable for use in cell therapy. These cells are clinically introduced into a tissue in an autologous or allogeneic manner for the treatment of inflammatory and degenerative diseases with or without gene therapy. There are however, some drawbacks related to the clinical use of MSCs, with one major limitation being that these cells tend to decrease in 'sternness' and quantity with increasing donor age and disease while the underlying mechanisms of stem cell ageing remain vague. An increased activity of the small RhoGTPase Cdc42 is thought to play a key modulatory role in MSC ageing. Cdc42 activity is known to partake in the regulation of numerous signaling pathways and also cause depolarization of planer cell polarity markers hence has been identified as a pharmacological target for improving MSC ageing. The aim of this study is to investigate the regulatory effect of small molecule on Cdc42 activity in MSCs

Protocol optimization for abtaining lyophilized human amniotic membrane extract

The amniotic membrane (AM) is the inner amniotic fluid-filled membrane which forms a cavity around the embryo. AM has long been used in the treatment of venous ulcers and burns. Freshly extracted AM has been shown to possess the highest effect, thus making the extraction method costly and severely limiting its possible usage. To counter this problem, several extraction methods have been developed aimed at preserving the growth factors present in AM and maintaining the AM in a sterile condition. The use of lyophilized AM extract could help preserve its active properties which is associated with the presence of a variety of cytokines necessary for wound-healing. However, there are no standard methods for obtaining amniotic-membrane extracts to date. We chose two available methods in which the resulting extract had the highest regenerative properties. Comparative analyses of these two methods were not carried out. Hence, in order to determine which method gave AM lyophilisates with the highest cytokine composition, we determined the cytokine content of the obtained lyophilisates with reference to that of the native membrane

3rd International scientific conference on regenerative medicine and healthy aging

Mesenchymal stem cells (MSCs) are an attractive stem cell source for transplantation. MSCs can easily differentiate into osteoblasts and increase in osteoblast number will have positive effect on the bone regeneration in such conditions as osteoporosis or Paget disease. To provide the targeted delivery of MSCs to bone we have synthesized a novel osteophilic polymer. The primary active sites of the polymer are bisphosphonate functional groups that target hydroxyapatite molecules (HA) on the bone surface. NHS groups on the other end of the molecule allow polymer to bind to the cell surface components. Coating of cell surfaces with the polymer would allow the cells to bind specifically to HA component of bone and localize the cellular repair functions to areas of injured bone

3rd International scientific conference on regenerative medicine and healthy aging

Mesenchymal stem cells (MSCs) are an attractive stem cell source for transplantation. MSCs can easily differentiate into osteoblasts and increase in osteoblast number will have positive effect on the bone regeneration in such conditions as osteoporosis or Paget disease. To provide the targeted delivery of MSCs to bone we have synthesized a novel osteophilic polymer. The primary active sites of the polymer are bisphosphonate functional groups that target hydroxyapatite molecules (HA) on the bone surface. NHS groups on the other end of the molecule allow polymer to bind to the cell surface components. Coating of cell surfaces with the polymer would allow the cells to bind specifically to HA component of bone and localize the cellular repair functions to areas of injured bone

Adiponectin plasma levels in patients with age-related dementia

Adiponectin play a significant role in the regulation of type 2 diabetes, obesity, atherosclerosis, non-alcoholic fatty liver disease and neurodegenerative disorders. Evidence suggests that adiponectin may be an independent risk factor for all-cause dementia and Alzheimer's disease (AD) and the level of adiponectin in plasma reflects its level in cerebrospinal fluid (CSF). Studies demonstrated that elevated adiponectin level in blood was associated with an increased risk of dementia and AD in women and indicates that the sex dimorphism with regard to adiponectin levels association with Alzheimer's disease was clearly observe