9 research outputs found

    НСйропротСктивныС свойства ΠΏΡ€ΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΠΎΠ³ΠΎ Ρ„ΡƒΡ€Π°Π½Π° - соСдинСния Π“Π˜Π–-276 Π½Π° ΠΌΠΎΠ΄Π΅Π»ΠΈ гСморрагичСского ΠΈΠ½ΡΡƒΠ»ΡŒΡ‚Π°

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    The study of the neuroprotective properties new original compound GIZH-276 and evaluate changes activities of prolyl endopeptidase (PEP) and lactate dehydrogenase (LDH) in blood plasma of rats with post-traumatic hematoma (model of hemorrhagic stroke) is the aim of this investigation. Methodology of the study: Modeling of hemorrhagic stroke (HS) was carried out using the creation of intracerebral post-traumatic hematoma with the introduction of autologous blood into the site of injury. Assay of enzymes activities in plasma was carried out with fluorescent (PEP) and ultraviolet (LDH) methods. The results of the study: GIZH-276 at dose of 10 mg/kg (7 days, intraperitoneally) has a pronounced neuroprotective effect, attenuating neurological deficits in rats with hemorrhagic stroke 24 hours, 7 and 14 days after surgery and statistically significantly increasing the number of surviving rats by 14 days of observation. The weakening of neurological deficit and death of animals is accompanied by decreased activity of enzymes LDH and PEP, which indicates the improvement of security of brain tissue oxygen and reducing neuroinflammation. Conclusion: Safety and neuroprotective effect of GIZH-276 on the model of hemorrhagic stroke may be partially due to positive effect on inflammatory and hypoxia during the development of secondary brain damage in stroke.ЦСлью настоящСго исслСдования Π±Ρ‹Π»ΠΎ ΠΈΠ·ΡƒΡ‡Π΅Π½ΠΈΠ΅ Π½Π΅ΠΉΡ€ΠΎΠΏΡ€ΠΎΡ‚Π΅ΠΊΡ‚ΠΈΠ²Π½Ρ‹Ρ… свойств Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΎΡ€ΠΈΠ³ΠΈΠ½Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ соСдинСния Π“Π˜Π–-276 с биохимичСской ΠΎΡ†Π΅Π½ΠΊΠΎΠΉ Π΅Π³ΠΎ влияния Π½Π° Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚ΠΎΠ² пролилэндСпСптидазы (ПЭП ΠΈ Π»Π°ΠΊΡ‚Π°Ρ‚Π΄Π΅Π³ΠΈΠ΄Ρ€ΠΎΠ³Π΅Π½Π°Π·Ρ‹ (Π›Π”Π“) Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡ€ΠΎΠ²ΠΈ крыс с посттравматичСской Π³Π΅ΠΌΠ°Ρ‚ΠΎΠΌΠΎΠΉ, модСлью гСморрагичСского ΠΈΠ½ΡΡƒΠ»ΡŒΡ‚Π°. ΠœΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠ° исслСдования: ΠΌΠΎΠ΄Π΅Π»ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠ΅ гСморрагичСского ΠΈΠ½ΡΡƒΠ»ΡŒΡ‚Π° ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½ΠΎ с использованиСм ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΈΠΊΠΈ создания ΠΈΠ½Ρ‚Ρ€Π°Ρ†Π΅Ρ€Π΅Π±Ρ€Π°Π»ΡŒΠ½ΠΎΠΉ посттравматичСской Π³Π΅ΠΌΠ°Ρ‚ΠΎΠΌΡ‹ с Π²Π²Π΅Π΄Π΅Π½ΠΈΠ΅ΠΌ Π°ΡƒΡ‚ΠΎΠΊΡ€ΠΎΠ²ΠΈ Π² мСсто поврСТдСния. ЀлуоримСтричСски, ΡƒΠ»ΡŒΡ‚Ρ€ΠΎΡ„ΠΈΠΎΠ»Π΅Ρ‚ΠΎΠ²Ρ‹ΠΌ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½ΠΎ биохимичСскоС исслСдованиС влияния Π“Π˜Π–-276 Π½Π° Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚ΠΎΠ² ПЭП ΠΈ Π›Π”Π“ Π² ΠΏΠ»Π°Π·ΠΌΠ΅ ΠΊΡ€ΠΎΠ²ΠΈ крыс с Π“Π˜. Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ исслСдования: выявлСно, Ρ‡Ρ‚ΠΎ Π“Π˜Π–-276 Π² Π΄ΠΎΠ·Π΅ 10 ΠΌΠ³/ΠΊΠ³ (7 Π΄Π½Π΅ΠΉ, Π²Π½ΡƒΡ‚Ρ€ΠΈΠ±Ρ€ΡŽΡˆΠΈΠ½Π½ΠΎ) ΠΎΠΊΠ°Π·Ρ‹Π²Π°Π΅Ρ‚ Π²Ρ‹Ρ€Π°ΠΆΠ΅Π½Π½Ρ‹ΠΉ Π½Π΅ΠΉΡ€ΠΎΠΏΡ€ΠΎΡ‚Π΅ΠΊΡ‚ΠΈΠ²Π½Ρ‹ΠΉ эффСкт, ослабляя нСврологичСский Π΄Π΅Ρ„ΠΈΡ†ΠΈΡ‚ Ρƒ крыс с гСморрагичСским ΠΈΠ½ΡΡƒΠ»ΡŒΡ‚ΠΎΠΌ Ρ‡Π΅Ρ€Π΅Π· 24 Ρ‡, 7 ΠΈ 14 сут. послС ΠΎΠΏΠ΅Ρ€Π°Ρ†ΠΈΠΈ ΠΈ статистичСски достовСрно увСличивая число Π²Ρ‹ΠΆΠΈΠ²ΡˆΠΈΡ… крыс ΠΊ 14-ΠΌ суткам наблюдСния. ОслаблСниС нСврологичСского Π΄Π΅Ρ„ΠΈΡ†ΠΈΡ‚Π° ΠΈ Π³ΠΈΠ±Π΅Π»ΠΈ ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ… сопровоТдаСтся сниТСниСм активности Ρ„Π΅Ρ€ΠΌΠ΅Π½Ρ‚ΠΎΠ² Π›Π”Π“ ΠΈ ПЭП, Ρ‡Ρ‚ΠΎ ΡΠ²ΠΈΠ΄Π΅Ρ‚Π΅Π»ΡŒΡΡ‚Π²ΡƒΠ΅Ρ‚ ΠΎΠ± ΡƒΠ»ΡƒΡ‡ΡˆΠ΅Π½ΠΈΠΈ обСспСчСния Ρ‚ΠΊΠ°Π½Π΅ΠΉ ΠΌΠΎΠ·Π³Π° кислородом ΠΈ ΡƒΠΌΠ΅Π½ΡŒΡˆΠ΅Π½ΠΈΠΈ нСйровоспалСния. Π—Π°ΠΊΠ»ΡŽΡ‡Π΅Π½ΠΈΠ΅: Π·Π°Ρ‰ΠΈΡ‚Π½Ρ‹ΠΉ, Π½Π΅ΠΉΡ€ΠΎΠΏΡ€ΠΎΡ‚Π΅ΠΊΡ‚ΠΈΠ²Π½Ρ‹ΠΉ эффСкт Π“Π˜Π–-276 Π½Π° ΠΌΠΎΠ΄Π΅Π»ΠΈ гСморрагичСского ΠΈΠ½ΡΡƒΠ»ΡŒΡ‚Π° ΠΌΠΎΠΆΠ΅Ρ‚ Π±Ρ‹Ρ‚ΡŒ частично обусловлСн Π΅Π³ΠΎ ΠΏΠΎΠ»ΠΎΠΆΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹ΠΌ влияниСм Π½Π° Π²ΠΎΡΠΏΠ°Π»ΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎΠ΅ ΠΈ гипоксичСскоС Π·Π²Π΅Π½ΠΎ развития Π²Ρ‚ΠΎΡ€ΠΈΡ‡Π½Ρ‹Ρ… ΠΏΠΎΠ²Ρ€Π΅ΠΆΠ΄Π΅Π½ΠΈΠΉ ΠΌΠΎΠ·Π³Π° ΠΏΡ€ΠΈ ΠΈΠ½ΡΡƒΠ»ΡŒΡ‚Π΅

    Tuning SPT-3G transition-edge-sensor electrical properties with a four-ayer Ti–Au–Ti–Au thin-film stack

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    We have developed superconducting Ti transition-edge sensors with Au protection layers on the top and bottom for the South Pole Telescope’s third-generation receiver (a cosmic microwave background polarimeter, due to be upgraded this austral summer of 2017/2018). The base Au layer (deposited on a thin Ti glue layer) isolates the Ti from any substrate effects; the top Au layer protects the Ti from oxidation during processing and subsequent use of the sensors. We control the transition temperature and normal resistance of the sensors by varying the sensor width and the relative thicknesses of the Ti and Au layers. The transition temperature is roughly six times more sensitive to the thickness of the base Au layer than to that of the top Au layer. The normal resistance is inversely proportional to sensor width for any given film configuration. For widths greater than five micrometers, the critical temperature is independent of width

    EFFECTS OF LEVETINOL ON EPILEPTIFORM ACTIVITY OF THE BRAIN IN RATS WITH COBALT-INDUCED EPILEPSY

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    The aim of this study was to evaluate the anti-seizure effect of Levetinol tablet (Geropharm) on the cobalt-induced chronic epilepsy.Materials and methods. A model of cobalt-induced epilepsy was created by applying cobalt powder to the sensorimotor zone of the rat cortex. The effects of Levetinol were studied at the early stage of the epileptic system (ES) formation (on day 2 after the cobalt application), and then at the stage of fully developed ES (on day 6 after the cobalt application).Results. The present study showed that at the early stage of ES development, Levetinol at doses of 50 and 200 mg/kg had no statistically significant effect on the development of paroxysmal activity in both primary and secondary epileptic foci: in the ipsi- and contralateral cortex, hypothalamus and hippocampus. On day 6 of the cobalt-induced epilepsy, a significant suppression of paroxysmal activity in the above structures of the brain was observed with the administration of Levetinol at a dose of 200 mg/kg. The most pronounced anti-seizure effect was found in the hippocampus; that was expressed in normalization of the bioelectrical activity and appearance of the regular theta rhythm.Conclusion. The effects of Levetinol are largely manifested in the hippocampal foci of epileptiform activity and, to a lesser extent, in the cortical foci

    4-BENZOYLPYRIDINE OXIME DERIVATIVE (GIZH-298) VERSUS VALPROIC ACID: THE ANTICONVULSANT POTENTIAL EFFECT IN A MODEL OF EPILEPSY IN RATS WITH COBALT-INDUCED LESIONS

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    Objective of the research is to evaluate the anticonvulsant effect of the new original compound GIZH-298 (4-benzoylpyridine oxime derivative) versus valproic acid (VPA) in a model of epilepsy in rats with cobalt-induced lesions.Materials and methods. Modeling of epileptic status was performed using the technique of creating a chronic epileptogenic focus caused by the application of cobalt to the sensorimotor zone of the rat cortex, followed by intraperitoneal administration of homolecysteine thiolactone. Compounds GIZH-298 and VPA were introduced against the background of development of electrographic status with behavioral convulsive seizure manifestations.Results. The study revealed that GIZH-298 at a dose of 60 mg/kg (i. p.) in 50 minutes after injection reduces the number of high-amplitude generalized discharges caused by homocysteine thiolactone in the ipsilateral and contralateral cortex (46-fold decrease), in the hippocampus and hypothalamus (28-fold decrease); eliminates (in 100% of the animals) the generalized tonic-clonic seizures that arise in the advanced stage of status epilepticus. VPA at a dose of 100 mg/kg (i. p.) in 3 hours after injection significantly suppresses the EpA in all evaluated structures with the maximum value in the hypothalamus (28-fold decrease), and after 5 hours in the ipsilateral and contralateral (33-fold decrease). At the same time, VPA eliminates generalized motility of status epilepticus only in 71% of the animals and protects from death 86% of the rats.Conclusion. The compound GIZH-298 significantly earlier (for 2 hours) than the VPA (100 mg/kg) and at a lower dose (60 mg/kg) fully eliminates electrographic (in all evaluated brain structures with the greatest efficiency in the contralateral cortex and the hypothalamus) and behavioral manifestations of unfolded status epilepticus and prevents deaths in 100%
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