Apical Leakage after Four Endodontic Instrumentation and Filling Procedures

Svrha je bila ispitati apeksnu propusnost punjenih korijenskih kanala uporabom konstrukcije za prijenos tekućine, nakon dvije različite tehnike instrumentacije (“double flare” i step-back”) kombinirane s uporabom dvaju različitih materijala za punjenje (eukaperka i Roeko seal®). U pokusu je rabljen uzorak od 40 trajnih jednokorijenskih zuba. Zubi su dekoronirani na razini caklinskocementnog spojišta, a zatim podijeljeni u četiri skupine od po 10 zuba. Korijenski kanali prve i druge skupine mehanički su obrađeni “double flare” tehnikom, a “stepback” tehnika primijenjena je u trećoj i četvrtoj skupini. kanali su tijekom instrumentacije ispirani 2,5% otopinom NaOCl. Obrađeni korijenski kanali prve i treće skupine punjeni su gutaperka kolčićima i eukaperka pastom tehnikom hladne alteralne kondenzacije, dok je u drugoj i četvrtoj skupini rabljen Roeko seal® umjesto eukaperke tijekom punjenja. Učinjenim endodontskim postupcima stvorene su četiri skupine: skupina DF/EF (double-flare / eukaperka) skupina DF/RS (double-flare / Roeko seal®) skupina, skupina SB/EP (step back/eukaperka) i skupina SB/RS (step back / Roeko seal®). Punjeni korijeni pohranjeni su u sterilnu fiziološku otopinu na 37°C u razdoblju od 7 dana nakon čega su postavljeni u konstrukciju za prijenos tekućine. Propusnost je mjerena pomakom zračnoga mjehurića u kapilarnoj cijevi spojenoj s apeksnim krajem napunjenoga zubnog korijena. Razlika između skupine DF/EF (0,152 μL ± 0,12), skupine DF/RS (0,186 μL ± 0,098), skupine SB/EP (0,195 μL ± 0,12), i skupine SB/RS (0,360 μL ± 0,230) statistički je analizirana Sheffe (ANOVA) testom. statistički znatna razlika utvrđena je između skupine DF/EP i skupine SB/RS. Daljnjom statističkom raščlambom s pomoću Mann-Whitney U testa utvrđeno je da različita tehnika instrumentacije utječe na rezultate raščlambe. U uvjetima pokusa, rezultati dobiveni primjenom konstrukcije za prijenos tekućine pokazuju da različiti materijali za punjenje korijenskih kanala ne utječu na apeksnu propusnost.The aim of this research was to examine apical obturation leakage of filled root canals by a fluid transport model after two different instrumentation techniques (“double flare” and “step-back”) combined with use of two different sealers (eucapercha and Roeko seal®). A sample of 40 permanent single-rooted teeth was used. The coronal part of each tooth was removed at the amelo-cement junction. Teeth were divided into four groups of 10 teeth each. Root-canals of the first and second group were cleaned and shaped by “double flare” technique while “step-back” technique was performed in the third and fourth group. All root-canals were irrigated with 2.5% NaOCl during instrumentation. Prepared root-canals of the first and third group were filled with gutta-percha points and eucapercha sealer by the cold lateral condensation technique while in the second and fourth group Roeko seal® was used as a sealer instead of eucapercha during filling procedure. Filled roots were stored in NaCl 3% at 37°C for 7 days. These procedures formed four groups: group DF/EO (double flare/eucapercha), group DF/RS (double flare/Roeko seal®, group SB/EP (step back/eucapercha) and group SB/RS (step back/Roeko seal®). Each root was mounted in a fluid transport model and leakage was measured by the movement of an air bubble in a capillary glass tube connected to the experimental root section. The difference between group DF/EP (0,152 μL ± 0,12), group DF/RS (0,186 μL ± 0,098), group SB/EP (0,195 μL ± 0,12), and group SB/RS (0,360 μL ± 0,230) was statistically analyzed by Sheffe test. Statiatically significant difference was determined between group DF/EO and group SB/RS. Further statistical anlysis peformed by Mann-Whitney U test showed that different instrumentation technique were responsable for the results of analysis. Under the conditions of this research, results obtained with fluid transport model indicate that there is no statistically significant difference between groups where rootcanals were filled with different materials. Apical leakage was signifuicantly lower where root-canals were instrumented by “double flare” technique