Environmental factors influence both abundance and genetic diversity in a widespread bird species.

Genetic diversity is one of the key evolutionary variables that correlate with population size, being of critical importance for population viability and the persistence of species. Genetic diversity can also have important ecological consequences within populations, and in turn, ecological factors may drive patterns of genetic diversity. However, the relationship between the genetic diversity of a population and how this interacts with ecological processes has so far only been investigated in a few studies. Here, we investigate the link between ecological factors, local population size, and allelic diversity, using a field study of a common bird species, the house sparrow (Passer domesticus). We studied sparrows outside the breeding season in a confined small valley dominated by dispersed farms and small-scale agriculture in southern France. Population surveys at 36 locations revealed that sparrows were more abundant in locations with high food availability. We then captured and genotyped 891 house sparrows at 10 microsatellite loci from a subset of these locations (N = 12). Population genetic analyses revealed weak genetic structure, where each locality represented a distinct substructure within the study area. We found that food availability was the main factor among others tested to influence the genetic structure between locations. These results suggest that ecological factors can have strong impacts on both population size per se and intrapopulation genetic variation even at a small scale. On a more general level, our data indicate that a patchy environment and low dispersal rate can result in fine-scale patterns of genetic diversity. Given the importance of genetic diversity for population viability, combining ecological and genetic data can help to identify factors limiting population size and determine the conservation potential of populations

Using Focus Groups in Preliminary Instrument Development: Expected and Unexpected Lessons Learned

Focus groups can be utilized effectively across various stages of instrument development. This article details selected aspects of a process in which they were employed at the initial stages of item generation and refinement in a study of occupational stereotyping. The process yielded rich contextual information about the worldview and corresponding terminology of participants. In addition, the use of a tool developed and previously employed as an approach to clinical case notes (i.e., SOAP notes), produced surprising benefits in documenting the focus group data. The purpose of this paper is to describe this process and highlight the insights that emerged. The process and outcomes have methodological implications for qualitative researchers conducting focus groups as well as for those developing new surveys, scales, and measurements

Deep Learning-Based Generation of Synthetic CT from MR Images for Craniosynostosis Planning

Craniosynostosis is a rare congenital defect caused by the premature fusion of one or more cranial sutures. This untimely cranial ossification hinders correct brain development. Its clinical diagnosis and treatment planning usually rely on Computed Tomography (CT), a potentially harmful imaging technique for young infants. It is with the intent of avoiding the use of ionizing radiation in this clinical pipeline that this work studies how feasible it is to resort to alternative non-detrimental imaging techniques such as Magnetic Resonance Imaging (MRI). We evaluate the performance of neural network generators trained on Generative Adversarial Networks in the MRI-to-CT translation task. We train nine generative models on 25 paired MR-CT medical scans, and validate and test their performance on 8 and 4 paired images, respectively. The results are promising both from qualitative and quantitative standpoints, particularly those of the models trained directly on 3D data. Results demonstrate that it is feasible to generate reliable and accurate synthetic CT scans from MR images with the proposed framework, opening up the possibility of harnessing the benefits of non-ionizing techniques to drive craniosynostosis diagnosis and treatment planning.CERMEP database has © Copyright CERMEP – Imagerie du vivant, www.cermep.fr and Hospices Civils de Lyon. All rights reserved. Research supported by projects PI122/00601 and AC20/00102 (Ministerio de Ciencia, Innovación y Universidades, Instituto de Salud Carlos III, Asociación Española Contra el Cáncer and European Regional Development Fund “Una manera de hacer Europa”), project PerPlanRT (under the frame of ERA PerMed), TED2021- 129392B-I00 and TED2021-132200B-I00 (MCIN/AEI/10.13039/501100011033 and European Union “NextGenerationEU”/PRTR)

Novel associations of UDP-glucuronosyltransferase 2B gene variants with prostate cancer risk in a multiethnic study.

BACKGROUND: We have previously shown that a functional polymorphism of the UGT2B15 gene (rs1902023) was associated with increased risk of prostate cancer (PC). Novel functional polymorphisms of the UGT2B17 and UGT2B15 genes have been recently characterized by in vitro assays but have not been evaluated in epidemiologic studies. METHODS: Fifteen functional SNPs of the UGT2B17 and UGT2B15 genes, including cis-acting UGT2B gene SNPs, were genotyped in African American and Caucasian men (233 PC cases and 342 controls). Regression models were used to analyze the association between SNPs and PC risk. RESULTS: After adjusting for race, age and BMI, we found that six UGT2B15 SNPs (rs4148269, rs3100, rs9994887, rs13112099, rs7686914 and rs7696472) were associated with an increased risk of PC in log-additive models (p < 0.05). A SNP cis-acting on UGT2B17 and UGT2B15 expression (rs17147338) was also associated with increased risk of prostate cancer (OR = 1.65, 95% CI = 1.00-2.70); while a stronger association among men with high Gleason sum was observed for SNPs rs4148269 and rs3100. CONCLUSIONS: Although small sample size limits inference, we report novel associations between UGT2B15 and UGT2B17 variants and PC risk. These associations with PC risk in men with high Gleason sum, more frequently found in African American men, support the relevance of genetic differences in the androgen metabolism pathway, which could explain, in part, the high incidence of PC among African American men. Larger studies are required

Heat shock proteins and exercise

Uno de mecanismos que posee el organismo para contrarrestar la ruptura de la homeostasis es la producción de proteínas de estrés o heat shock proteins (HSP). Aumentos en la temperatura corporal, en la concentración de Ca2+ y de ciertos corticoides así como disminuciones del pH, de la glucemia y del glucógeno se presentan, junto a la isquemia, como los principales agentes desencadenantes de la respuesta de HSP. La práctica de ejercicio físico integra gran parte de estos factores, lo que provoca una clara liberación de HSP muscular tras diferentes tipos de esfuerzos. Factores como la edad, el género, el nivel de entrenamiento, la intensidad y el volumen del ejercicio realizado o el tipo de fibra muscular, parecen tener una relación directa con las adaptaciones biológicas relacionadas con las HSP. Son necesarios más estudios para definir con mayor exactitud la función biológica de las HSP vinculada con el ejercicio físico

Using whole body vibration to improve jump ability in young recreational sportmen

The whole body vibrations (WBV) it is nowadays one of the most widely used methods for improvingthe explosive strength. In this study, 12 subjects participating in recreational physical activity were allocated to a5 sets of 60 s training, using a frequency of 30 Hz, an amplitude of 2.5 mm. and a isometric position (110 °bending knees) on a Galileo Fitness ® (Novotech, Germany) platform. The results showed an increase in SJ(+1.76 ± 4.05 cm) and CMJ (+1.10 ± 3.20 cm) in the post-test conducted just after the vibration. The values of the post-test performed 30 minutes after the squat jump remained above the ones of pre-test but just below the ones of the immediate post-test (+0.42 ± 4.43 cm). By contrast the values in the counter movement jump drop below the pre-test ones (-0.12 ± 2.45 cm). Based on these data it seems that when the frequency is not high it is necessary to use a greater amplitude in order to achieve the desired effects. The effect achieved after the vibration is transient, not remaining after 30 minutes

HIV test-seeking before and after the restriction of anonymous testing in North Carolina.

OBJECTIVES: This study assessed the impact on HIV test-seeking of North Carolina's restriction of anonymous testing to 18 of its 100 counties as of September 1, 1991. METHODS: Trends from 4 months prerestriction to the 16-month restriction period in counties retaining vs counties eliminating anonymous testing were compared. RESULTS: HIV testing increased throughout the state, but more rapidly where anonymous testing was retained than elsewhere: 64% vs 44%. These differences held for all sociodemographic subgroups and were most pronounced among adolescents and African Americans and other non-Whites. CONCLUSIONS: The data are consistent with a detrimental effect of elimination of anonymous testing, although confounding from differences in AIDS awareness or in repeat tests is possible