Wplyw cyklosporyny A na przebieg miesniowej fazy wlosnicy u myszy

The authors tried to determine the role of TH lymphocytes in the course of muscular trichinellosis in mice. The investigations were carried out on the CFW mice infected with 200 Trichinella spiralis larvae and treated with Cyclosporine A to suppresse TH1 lymphocytes. In animals treated with the drug more numerous cells inside the larval capsules and more numerous granulomas compared to control were seen. At the same time the mean number of larvae was lower (69,2%) than in control. The results show that TH1 lymphocytes do not play the significant role in the destruction of muscular larvae. This is the first conclusion and requires of course futher investigations

INFLUENCE OF CYCLOSPORINE A ON THE COURSE OF MUSCULAR PHASE OF TRICHINELLOSIS IN MICE

The authors tried to determine the role of TH lymphocytes in the course of muscular trichinellosis in mice. The investigations were carried out on the CFW mice infected with 200 Trichinella spiralis larvae and treated with Cyclosporine A to suppresse TH1 lymphocytes. In animals treated with the drug more numerous cells inside the larval capsules and more numerous granulomas compared to control were seen. At the same time the mean number of larvae was lower (69,2%) than in control. The results show that TH1 lymphocytes do not play the significant role in the destruction of muscular larvae. This is the first conclusion and requires of course futher investigations

ZJAWISKO APOPTOZY W PRZEBIEGU DOŚWIADCZALNEJ WŁOŚNICY U MYSZY

Mice infected with 200 Trichinella spiralis larvae were killed at 3-69 days post infection (dpi) and the jejunum and masseter muscles were sectioned in a cryostat and examined in the Tuneli method with "In situ Cell Detected Kid POD" of Boehringer-Mannheim. Data concering the exact localization and dynamie of apoptic cells in both organs are presented. The authors conclude that apoptosis plays a important role in the pathogenesis of trichinellosis

The cells observed inside capsules of larvae in the course of experimental trichinellosis in mice

In the course of trichinellosis in mice the numerous cells inside larval capsules were observed. Beginning on 14 day post infection (dpi) they were seen in about 7% of infected muscles fibers but at 21 dpi the percentage of capsules with the cells amounted to 77. In the next stage of infection the number of capsules containing cells continued to increase and reach almost 100% at 60 dpi. The identification of the cells was carried out by the indirect immunoenzymatic method using anti-CD4⁺, anti-CD8⁺ and anti-macrophage (Mac-1 antigen) monoclonal antibodies. In course of observation the level of CD4⁺ cells decreased from 11.3 to 0.7, of CD8⁺ from 17.7 to 3.0 and of macrophages from 71.0 to 23.1 %. The most numerous CD4⁺ cells were seen at 14 dpi while the CD8⁺ and macrophages at 21 dpi. Beginning on 28 dpi negatively reacting cells inside larval capsules were also observed. The number of these cells increased from 23.8 to 73.2% at 60 dpi and their identification requires further investigations. The role and the function of all these cells are discussed

The phenomenon of apoptosis in the course of experimental trichinellosis in mice

Mice infected with 200 Trichinella spiralis larvae were killed at 3-69 days post infection (dpi) and the jejunum and masseter muscles were sectioned in a cryostat and examined in the Tuneli method with "In situ Cell Detected Kid POD" of Boehringer-Mannheim. Data concering the exact localization and dynamie of apoptic cells in both organs are presented. The authors conclude that apoptosis plays a important role in the pathogenesis of trichinellosis