Studies on Uveitis Part II: Hypotheses with case reports

A number of patients with and without uveitis are used to show that this study of aqueous microbiology suggests the following hypotheses: 1. Routine techniques currently used in most microbiology laboratories overlook classical and variant bacteria which might be isolated from clinical specimens with improved microbiological techniques. 2. Bacterial forms may be present in other ocular tissue when not present in the aqueous. 3. Polymicrobial infections probably occur rather frequently, especially in chronic or subacute inflammatory states. 4. Alterations in various host factors may subject some persons to a greater incidence of inflammatory episodes including uveitis. 5. Antibiotics used in a regimen similar to that advocated to reduce recurrences of chronic Staphylococcal or Streptococcal disease may ameliorate the pattern of uveitis in some patients, especially if such regimens take into account the cell wall defective forms of these organisms. 6. The eye in some uveitic situations may be as resistant to microbial eradication by chemotherapeutic or biologic mechanisms as is the kidney in glomerulonephritis or the bone in osteomyelitis. 7. Sarcoid and its attendant uveitis may be related to a variant Mycobacterium species

A Microtechnique for the Isolation of Cell Wall Deficient Organisms from Very Small Samples

A miniaturized procedure using a modified microtiter plate is described for the isolation of cell wall deficient organisms. It is particularly useful when the volume of a liquid specimen is very small

Ocular Irrigating Solutions: A Comparison Between Balanced Salt Solution and L-410 (PO-EIS)

The ability of two ocular irrigating solutions to reduce postoperative corneal edema after intracapsular cataract extraction was compared using ultrasonic pachymetry. All patients had anterior chamber intraocular lenses implanted. The solutions were balanced salt solution and solution L-410 (PO-EIS), an eye irrigation solution containing dextran 40 and bicarbonate. Corneas irrigated with either solution showed no significant difference in postoperative edema. The use of L-410 (as an alternative to balanced salt solution) may not be necessary in intracapsular cataract extraction

Aqueous Microbiology: The Possible Role of Cell Wall Deficient Bacteria in Uveitis

Samples of aqueous humor were obtained from the anterior chamber of patients with uveitis. Bacterial L-forms, or cell wall deficient forms, were recovered from 11 of 19 eyes with unexplained uveitis. These bacterial variants were cultured on newer, highly specialized media and stained with acridine orange. This stain demonstrates fluorescence of nucleic acids under UV microscopy

Use of the Intraocular Lens in the Aphakic Eye - Part 1: The anterior chamber intraocular lens

Th;s paper reviews some reasons why many ophthalmic surgeons do not implant intraocular lenses. It presents some parameters of a theoretically ideal lens, some of the disadvantages and advantages of the currently available anterior chamber intraocular lenses, and lastly, it offers designs for a new anterior chamber intraocular lens. While preserving the advantages of currently available anterior chamber intraocular lenses, the new designs may have certain advantages. Currently under investigation, the new lenses may be available for use in man within the next year

Studies on uveitis Part I: Aqueous studies

Aqueous microbiological and cytological studies were performed on 134 anterior chamber taps done on 118 patients both with and without uveitis. Cell wall defective bacterial forms (CWDF) were recovered from about 40% of these aqueous specimens and classical bacteria were recovered from 12%. A statistically significant difference could not be found between the incidence of bacterial cell wall defective forms in the various anatomical types of uveitis. There were hints from this work that the presence of CWDF In the aqueous was more frequent in younger patients with uveitis, early in the uveitis attack, if there were multiple foci of infection, or if there was a history of arthritis, sarcoid, or diabetes. Neither the number of previous attacks of uveitis nor the sex of the patient seemed related to the presence of CWDF The slit-lamp presence of many cells, dense aqueous flare, or of hypopyon appeared significant, as did the presence of mutton fat KP. The presence of aqueous CWDF seemed to be related to the presence of certain leukocytes