49 research outputs found
Expression of the SST receptor 2 in uveal melanoma is not a prognostic marker
Introduction: Uveal melanoma (UM) cells and neurohormone-producing cells both originate from the neural crest. Somatostatin receptors subtype 2 (SSTR2) are over-expressed in several tumors, often from neuroendocrine origin, and synthetic antagonists like octreotide and octreotate are being used as diagnostic or therapeutic agents. We investigated the SSTR2 expression in UM, and determined whether this expression was related to prognosis of the disease. Materials and methods: UM cell lines and fresh primary UM samples were tested for SSTR2 expression by autoradiography (AR) using 125I-Tyr3-octreotate. Furthermore, UM cell lines were analyzed for SSTR2 mRNA expression with quantitative real-time RT-PCR. Results: Using AR, cell-surface SSTR2 expression was demonstrated in two UM metastatic cell lines, but no expression was detected in three cell lines derived from primary UM. However, all primary and metastatic UM cell lines showed mRNA expression levels for SSTR2 using quantitative real-time RT-PCR. Only three of 14 primary UM demonstrated moderate SSTR2 expression, and this expression was not significantly associated with tumor-free survival or any tested prognostic factor. Conclusions: Based on the rare and low expression of SSTR2 found in primary UM specimens and in UM cell lines, we conclude that SSTR2 is not widely expressed in UM. Furthermore, SSTR2 expression was not associated with tumor-free survival and prognostic factors. Therefore SSTR2 is not suited as prognostic marker or therapeutic target in UM
An Evaluation of Column-to-Column Retention Variability for Compounds Related to Panadiplon (U-78,875) Using Three Different Reverse-Phase HPLC Separation Modes
Bifeprunox: Relationship between antipsychotic potential, EPS liability and dopamine D2 receptor occupancy in rats
The association between antipsychotic capability, EPS incidence, and dopamine D-2 receptor occupancy of bifeprunox in rats
Symbolic Class Struggles and the Intersection of Socioeconomic, Cultural and Moral Categorisations
COMPARING DISTRIBUTIONS OF SHELF LIVES FOR DRUG PRODUCTS WITH TWO COMPONENTS UNDER DIFFERENT DESIGNS
RAPESEED REFERENCE MATERIALS FOR GLUCOSINOLATE ANALYSIS - DEVELOPMENT OF RAPESEED BCR RM-190 AND THE RESULTS OF THE INTERCOMPARISON OF METHODS
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