121 research outputs found
Role for the Mammalian Swi5-Sfr1 Complex in DNA Strand Break Repair through Homologous Recombination
In fission yeast, the Swi5-Sfr1 complex plays an important role in homologous recombination (HR), a pathway crucial for the maintenance of genomic integrity. Here we identify and characterize mammalian Swi5 and Sfr1 homologues. Mouse Swi5 and Sfr1 are nuclear proteins that form a complex in vivo and in vitro. Swi5 interacts in vitro with Rad51, the DNA strand-exchange protein which functions during HR. By generating Swi5β/β and Sfr1β/β embryonic stem cell lines, we found that both proteins are mutually interdependent for their stability. Importantly, the Swi5-Sfr1 complex plays a role in HR when Rad51 function is perturbed in vivo by expression of a BRC peptide from BRCA2. Swi5β/β and Sfr1β/β cells are selectively sensitive to agents that cause DNA strand breaks, in particular ionizing radiation, camptothecin, and the Parp inhibitor olaparib. Consistent with a role in HR, sister chromatid exchange induced by Parp inhibition is attenuated in Swi5β/β and Sfr1β/β cells, and chromosome aberrations are increased. Thus, Swi5-Sfr1 is a newly identified complex required for genomic integrity in mammalian cells with a specific role in the repair of DNA strand breaks
Crosslinking of viral nanoparticles with βclickableβ fluorescent crosslinkers at the interface
Hyaluronic acid fillers for correcting midface volume deficit in BarraquerβSimons syndrome
IgG is bound by antigen-antibody bonds and some IgG and albumin are bound by intermolecular disulfide bonds to cartilage in rheumatoid arthritis and osteoarthritis
Porcine heart valves produce a protein that induces cell-mediated connective tissue degradation: I. Biological properties.
The normal occurrence of two molecular forms of the eight complement component (C8) and their concentrations in a family with C8 deficiency
Porcine heart valves produce a protein that induces cell-mediated connective tissue degradation: II. Biochemical properties of the partially purified protein.
Passive Role of Articular Chondrocytes in the Pathogenesis of Acute Meniscectomy-induced Cartilage Degeneration
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