Association of VEGF gene +405C/G polymorphism with the risk of breast cancer in northern Iran

Background and Objective: Breast cancer is a cancer in women with high prevalancy worldwide. Vascular endothelial growth factor (VEGF) is one of the most important Pro-angiogenic factors. +405C/G is one of the common VEGF polymorphism which may have an impact on the level of gene expression and over loading of gene products. This study was done to evaluate the association between VEGF +405C/G gene polymorphism and breast cancer risk in north of Iran. Methods: This case-control study was carried out on 50 patients with breast cancer and 50 normal aged-matched controls in north of Iran. Genomic DNA was extracted from peripheral blood cells. To determine the genotype of +405 C/G VEGF gene polymorphism, PCR-RFLP method was used. Results: The prevalence of genotypic frequencies of GG, GC and CC in controls were 42%, 48% and 10%, respectively  and in patients were 22%, 46% and 32%, respectively (P<0.05). The +405C allele was considered as a risk factor in breast cancer (P<0.05). Conclusion: It seems +405 C/G VEGF gene polymorphism may be associated with the breast cancer in northern Iran

The association of ApE1 gene Asp148Glu polymorphism and breast cancer risk in Guilan population

Abstract Background: Breast cancer is the most common cancer and one of the main causes of cancer-related death all over the world that has become a major public health concern. Human apurinic/apyrimidinic endonuclease (ApE1) is a multifunctional protein that has an important role in the base excision repair (BER) pathway. Single- nucleotide polymorphism T>G found in exon 5 led to substitution of Asp>Glu at codon 148 (Asp148Glu). In this case-control study, we aimed to evaluate the association of this polymorphism on the risk of breast cancer in Guilan population. Materials and Methods: To study gene polymorphism APE1 (Case- Control), blood samples were collected from 75 patients diagnosed with breast cancer and 75 control subjects, and genotyped by allele-specific PCR (AS-PCR). To estimate the association between genotype and allele frequencies in cases and controls, Chi-Square (χ2) analysis was used. Results: Analysis revealed no significant differences were found in genotype and allele distributions of ApE1 Asp148Glu polymorphism between breast cancer patients and controls (p=0.1, p=0.6 respectively) in this population. Conclusion: Data from this study indicated no significant association between the Asp148Glu polymorphism and breast cancer risk (p=0.1). Further study needed to clarify the impact of Asp148Glu polymorphism on the breast cancer