Generation of a large scale repertoire of Expressed Sequence Tags (ESTs) from normalised rainbow trout cDNA libraries

BACKGROUND: Within the framework of a genomics project on livestock species (AGENAE), we initiated a high-throughput DNA sequencing program of Expressed Sequence Tags (ESTs) in rainbow trout, Oncorhynchus mykiss. RESULTS: We constructed three cDNA libraries including one highly complex pooled-tissue library. These libraries were normalized and subtracted to reduce clone redundancy. ESTs sequences were produced, and 96 472 ESTs corresponding to high quality sequence reads were released on the international database, currently representing 42.5% of the overall sequence knowledge in this species. All these EST sequences and other publicly available ESTs in rainbow trout have been included on a publicly available Website (SIGENAE) and have been clustered into a total of 52 930 clusters of putative transcripts groups, including 24 616 singletons. 57.1% of these 52 930 clusters are represented by at least one Agenae EST and 14 343 clusters (27.1%) are only composed by Agenae ESTs. Sequence analysis also reveals that normalization and especially subtraction were effective in decreasing redundancy, and that the pooled-tissue library was representative of the initial tissue complexity. CONCLUSION: Due to present work on the construction of rainbow trout normalized cDNA libraries and their extensive sequencing, along with other large scale sequencing programs, rainbow trout is now one of the major fish models in term of EST sequences available in a public database, just after Zebrafish, Danio rerio. This information is now used for the selection of a non redundant set of clones for producing DNA micro-arrays in order to examine global gene expression

Projet pilote "Mali-Sud" bas-fond de Kambo : rapport de synthèse : hydrologie-hydrogéologie

L'objectif de l'étude faite dans ce rapport sur le projet pilote de Kambo vise à quantifier le bilan hydrologique d'un bas-fond jugé représentatif de la région sur des critères morphologiques, pédologiques et climatiques en vue d'une meilleure mise en valeur agricole. La mise en évidence des paramètres déterminants du processus hydraulique doit permettre un avis objectif sur l'efficacité de l'aménagement du bas fond étudié et la mise au point d'un outil de diagnostic et d'aide à la décision pour l'aménagement des autres bas fonds de la régio