Incorporation of radiophosphate in the inorganic and organic acid soluble phosphate fractions of the rat sciatic nerve during Wallerian degeneration. I. After nerve section (neurotmesis)

Plasma inorganic phosphate incorporation into inorganic and organic acid soluble phosphate fractions of the rat sciatic nerve increased markedly during Wallerian degeneration. This high level of incorporation persisted from the 3rd to the 30th day of degeneration. These experiments confirm the possibility of an axonal inhibitory control on the Schwann cell metabolism.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

DEGENERESCENCE WALLERIENNE DU NERF SCIATIQUE DU RAT. ABSENCE D'ACTION DE LA CYCLOHEXIMIDE ET DE L'ACTINOMYCINE D SUR L'ACCROISSEMENT DE L'INCORPORATION DU RADIOPHOSPHATE DANS LES PHOSPHATES ACIDOSOLUBLES OBSERVE APRES SECTION DU NERF

Plasma inorganic phosphate incorporation into inorganic and organic acid soluble phosphate fractions of the rat sciatic nerve increases markedly during Wallerian degeneration. Administration of cycloheximide and actinomycin D remains without effect on this specific increase which appears thus independent of cytoplasmic protein neosynthesis.SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe

DEGENERESCENCE TRAUMATIQUE ET DEGENERESCENCE WALLERIENNE DU NERF SCIATIQUE DE RAT. INCORPORATION DU RADIOPHOSPHATE DANS LES PHOSPHATES ACIDOSOLUBLES DES SEGMENTS PROXIMAL ET DISTAL APRES SECTION DU NERF

In the central stump, the incorporation of plasma radiophosphate in acid soluble phosphates of nerve segments is increased within 1 hr after nerve section. The area of increased incorporation extends more centrally than the area of morphological changes. Traumatic degeneration in the central stump is not to be considered as a retrograde Wallerian degeneration.SCOPUS: NotDefined.jinfo:eu-repo/semantics/publishe

APERCU SUR LA PATHOGENIE ET LE TRAITEMENT DE L'OEDEME CEREBRAL

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Réparation du nerf périphérique :intérêt des colles biologiques et de la suture épipérineurale dans les interventions tardives. Etude expérimentale chez le rat

In order to approximate as close as possible genuine clinical conditions, the sciatic nerve of the rat was divided and then repaired after a delay of one or seven days either by application of a biological glue or by an epiperineural suture technique. The metabolic activity of the sciatic nerve Schwann cells - whether located in the distal or the proximal ends - and that of the (fast acting) white gastrocnemius and (slow acting) red soleus muscle were assessed using 32P-radiolabeled acid-soluble phosphates. Delayed repair, as judged by our biochemical criteria, was equivalent whatever method, biological glue or suture, was used. The frozen and lyophilized forms of the biological glue provided similar results. © 1985 Société d'Édition de l'Association d'Enseignement Médical des Hôpitaux de Paris.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Effect of increasing duration of denervation on the rate of entry of inorganic phosphate into rat gastrocnemius muscle

SCOPUS: ar.jinfo:eu-repo/semantics/publishe

INCORPORATION DU RADIOPHOSPHATE PLASMATIQUE DANS LES FRACTIONS PHOSPHATES MINERAL, ORGANIQUES ACIDO SOLUBLES ET PHOSPHOLIPIDIQUES DES NERFS SCIATIQUES DE LAPIN, NORMAL ET EN VOIE DE DEGENERESCENCE WALLERIENNE

Inorganic phosphate exchanges between plasma and sciatic nerve were measured in the rabbit using a 32PO4 tracer technique. Inorganic phosphate is taken up at the rate of 0.13 μg per hr and per 100 mg fresh weight. Incorporation of plasma radiophosphate is markedly increased into the inorganic acid soluble phosphate fractions of the distal part of the sectioned sciatic nerve. This increase is already significant within 1 hr after surgicial division, spreading at least 3 cm distally within 6 hr. This high level of incorporation persists until the 29th day of degeneration. These results favour the hypothesis that the axonal continuity maintains the metabolic activity of the Schwann cells at an inframaximal level. The authors confirm the rapid decrease in total phospholipid concentration in the nerve undergoing Wallerian degeneration as well as the marked increase in their specific activity. The authors show however that this increase in specific activity is due partly to the increased specific activities of the precursors (organic acid soluble phosphates), partly to the disappearance of a metabolically inert pool (myelin phospholipids). The Schwann cells of the nerve undergoing Wallerian degeneration do not have a more active phospholipid metabolism than their normal counterparts.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Sutures of fibrin glue for divided rat nerves. Schwann cell and muscle metabolism

A comparative study using 32P-labeling techniques on Schwann cell and skeletal muscle metabolism after repair of divided rat sciatic nerve by anastomosis with either microneurosurgical sutures or fibrinogen tissue adhesive shows no significant difference between the two repair modes. The successful measurement of functional muscle regeneration in the experimental study of nerve repair techniques is still hampered by the absence of definitive functional and physiologic criteria, despite an apparent normal anatomic restitution of the muscle.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Nerve and muscle regeneration after axonal division in rats treated with isaxonine phosphate (isopropylamino-2-pyrimidine phosphate)

Isaxonine phosphate (isopropylamino-2-pyrimidine phosphate), a neurotrophic agent, was tested on Schwann cell and muscle metabolism as evidenced by radiophosphate uptake in the inorganic phosphate and organic-bound aacid-soluble phosphate fractions. It had no demonstrable effect either on sciatic nerve Schwann cells above and below an experimental crush or on the corresponding gastrocnemius and soleus muscles in their denervated and reinnervated state (7th to 28th postoperative day). It also failed to influence the end results (150th postoperative day) at nerve and muscle level after surgical division of the nerve and repair by either microsuture or fibrinogen adhesive.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Alkaline phosphatase activities in normal and denervated, slow and fast rat muscles. Bearing on the possible participation of the enzyme in the transmembrane transport of phosphate ions.

Alkaline phosphatase (AP) activities are present in rat gastrocnemius:48.7, plantaris: 68.9, tibialis anterior: 69.1 and soleus: 96.7 nmol phenol. min-1. 100 mg muscles-1. These concentrations are one and two orders of magnitude lower than those observed in duodenum and placenta, but similar to those observed in liver. Response to activators/inhibitors and electrophoretic behaviour assign the muscle AP activities to the rat liver/placenta isoenzyme group. Motor denervation does not affect significantly the total muscle AP content within the first 30 postoperative days, however the concomitant variations in muscle weight are responsible for wide differences in AP concentrations between innervated, denervated and reinnervated muscles. Parallel determinations of radiophosphate uptake and AP activities failed to document a necessary link between the two variables, i.e. between enzyme concentration and phosphate ion transport.SCOPUS: ar.jinfo:eu-repo/semantics/publishe