Comparison of Antioxidant and Antihyaluronidase Activities in Extract of Basil Leaves (Ocimum americanum L.) with Eugenol Compound

Aging is a slow progressive loss of the ability of tissue to repair or regenerate and maintain its normal structure and function. Free radicals has been one of the aging factor, because free radicals can damage molecule in the body like lipid and protein. Antioxidant can help protect the body from free radicals. Has been known from many studies that there is many fruits and vegetables that contain antioxidant and antiaging (antihyaluronidase). One of that is basil leaves (Ocimum americanum L.). And one of the compound which can be found quite much in basil leaves is eugenol. This study aims to reveal comparison of the antioxidant and antihyaluronidase potential possessed in etanol extract of basil leaves compare to Eugenol compound. The hydrogen peroxide (H2O2) radicals scavanging antioxidant activity was measured based on the reaction method of ferrous ammonium sulphate and phenanthroline with little modification. The antihyaluronidase activity was detected by measuring the amount of Hyaluronic Acid based on method that has been told by Sigma Aldrich and Tu & Tawata (2015) with little modification. Eugenol had an IC50 value for H2O2  radicals scavenging activity as of 100,82 ± 8.60 µg/mL and basil leaves extract was 171,68 ± 2.21 µg/mL. While hyaluronidase inhibition activity from eugenol was 26,69 ± 2.89 µg/mL and basil leaves extract was 65,37 ± 3.96 µg/mL. In summary, eugenol compound which can be found in extract of basil leaves (Ocimum americanum L.) has better H2O2 radicals scavanging activity and  hyaluronidase enzyme inhibitory activity than the extract of basil leaves (Ocimum americanum L.). Thus, present study described maybe eugenol is the main antioxidant and antiaging potential compound  in basil leaves extract

Comparison Activities of Peel and Extract of Lime (Citrus amblycarpa) as Antioxidant and Antielastase

Aging of the skin is a physiological process that occurs in all living things, along with increasing age, hormones, nutrition, and exposure to sunlight both directly and indirectly. This process will form Reactive Oxygen Species ROS, free radicals that interfere with one of the most important enzymes in the cell matrix, namely the elastase enzyme. Free radicals can be neutralized by the presence of antioxidants. Lime plants have natural phytochemical compounds that act as antioxidants and anti-aging. This study was to compare the antioxidant activity and anti-elastase from the peel and lime seed extract (Citrus amblycarpa) by conducting H2O2 trapping tests and inhibition of elastase. The test results of antioxidant activity and anti-aging trapping of H2O2 lime seed extract had an IC50 value lower than lime peel extract, with yields of 194.82 μg / ml better than those of lime skin extract of 246.84 μg / ml. The results of the elastase inhibition activity test showed that lime peel extract had a lower IC50 value of 36.94 μg / ml compared to lime seed extract with an IC50 value of 62.39 μg / ml. A lime seed extract has antioxidant activity through better H2O2 trapping compared to lime peel extract while lime skin extract has antiaging activity through inhibition of elastase which is better than lime seed extract