Effect of cisplatin drug on nCLU-U7 LNCaP cells.

<p>(A) LNCaP cells were treated at the indicated doses for 24 hours and proliferation was assessed. (*): P value <0.05 (Student’s t-test) vs control cells. (B) Effect of cisplatin treatment on DNA fragmentation of LNCaP cells. LNCaP cells were treated with 50 µM cisplatin for 0, 4 and 24 hours. Cellular DNA was isolated and subjected to agarose gel electrophoresis.</p

nClu expression led to defective interaction between Bax and Ku70.

<p>(A) Coimmunoprecipitation of Bax and Ku70 following or not irradiation. No Bax- Ku70 coimmunoprecipitation was found at time 0 in not irradiated U7-nClu cells compare to U7 control cells. No coimmunoprecipitation of Bax and Ku70 was observed in all irradiated cells both at 4 hrs (not shown) and 24 hrs. (B) Defective expression of KU70 in nCLU-transduced cells. Note the upregulation of Rad17 protein following irradiation of nCLU-LNCap.</p

U7-nCLU vector construct and CLU mRNA expression in LNCaP.

<p>(A) (Top) Antisens sequence position to induce exon 2 skipping. (Bottom) Structure of the pHIV-1-U7-nCLU. U7snRNA including antisens sequence is placed under the control of its own promoter and its regulating domains 3′. SmOPT: sequence indicates the Sm protein binding site. LTR : long terminal repeats. (B) U7 vector integration in transduced LNCaP cell genome. Curves represent qPCR amplification of U7-nCLU genomic DNA in different transduction conditions (middle curves). Human β-actin amplification corresponded to positive control (Top curves) and genomic DNA of non transduced cells was used as negative control. (C) (Top) mRNA from transduced LNCaP cells with U7-nCLU vector was analyzed by RT-PCR at 3 weeks posttransduction. The 344 bp and 218 bp bands correspond to sCLU and skipped form nCLU mRNA respectively without alteration of the sequence. Controls were from RNA of LNCaP alone or LNCaP transduced with U7-control. (Bottom) Relative mRNA expression levels determined by densitometry. βactin used to normalize changes in specific gene expressions. (D) DNA sequence of the 218 bp band showed skipping of exon 2.</p

Increased UV-C induced death following U7-nCLU vector expression in LNCaP cells.

<p>FACS analysis was performed at 0, 2 and 4 hours after 10 min UV-C cell exposure. Cell death was assessed using propidium iodide (PI)/annexin V staining. The red rectangle surrounds PI−/Annexin V+cells. Histogram represent % of dead cells in each condition. Results are means +/− s.e.m. from 3 individual experiments. (*): P value <0.05 (Student’s t-test).</p

Responses of anti-CII T hybridomas upon stimulation with chemically modified CII256–270 glycopeptides

<p><b>Copyright information:</b></p><p>Taken from "Insights into spatial configuration of a galactosylated epitope required to trigger arthritogenic T-cell receptors specific for the sugar moiety"</p><p>http://arthritis-research.com/content/9/5/R92</p><p>Arthritis Research & Therapy 2007;9(5):R92-R92.</p><p>Published online 11 Sep 2007</p><p>PMCID:PMC2212564.</p><p></p> Data are expressed as means of two to four individual experiments. Blocking effects of alterations reaching the ε-primary amino group of Hylor strongly affecting the stereochemical position of sugar moiety. CII, collagen type II; CII256–270, immunodominant epitope of bovine type II collagen

Epistasis between <i>BANK1</i> rs3733197 and <i>BLK</i> rs13277113.

<p>OR for trend for the G allele of <i>BANK1</i> rs3733197 and the three <i>BLK</i> rs13277113 genotypes by population (France, Spain and Japan).</p

Results of single marker analysis in populations genotyped for both <i>BANK1</i> and <i>BLK</i>.

<p>Number of cases and controls, frequency of G allele, odds ratio (OR) and 95% confidence interval (95% CI) and <i>P</i>-value of the association under a multiplicative model for each population separately, for European Caucasians and for the combined dataset (France, Spain and Japan).</p

Epistasis between <i>BANK1</i> rs3733197 and <i>BLK</i> rs13277113 in rheumatoid arthritis: association of BANK rs3733197 G allele and BLK rs13277113 genotypes.

<p>Freq: frequency of the <i>BANK1</i> rs3733197 G risk allele, OR [95%CI]: odds ratio, 95% confidence interval.</p>*<p>France, Spain and Japan combined.</p

Distribution of BLK-rs13277113 and BANK1-rs3733197 multilocus genotypes in cases and control populations from Spain, France and Japan.

<p>Number of cases and controls, beta coefficient for logistic regression, corresponding p-value and risk category (“H” for high risk, “L” for low risk and “O” for unclassified) from Model Based Multifactor Dimensionality Reduction analysis for each multilocus genotype.</p

Meta-analysis of association of <i>BANK1</i> alleles and rheumatoid arthritis.

<p>Number of cases and controls; frequency of the G allele in cases and controls; and OR, 95% CI and <i>P</i>-value for the association for each population separately and for European Caucasians and non-European Caucasians and the combined dataset. All data were obtained from the literature, except for the France and Japan data, for which new genotyping was performed for this study.</p