2 research outputs found
Chloramphenicol-Incorporated Poly Lactide-co-Glycolide (PLGA) Nanoparticles: Formulation, Characterization, Technetium-99m Labeling and Biodistribution Studies
Chloramphenicol-loaded (CHL) poly-D,L-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) were prepared by
emulsification solvent evaporation technique either by using polyvinyl alcohol (PVA) as emulsion stabilizer or polysorbate-
80 (PS-80) as surfactant and characterised by transmission electron microscopy, zeta-potential measurements. The NPs were
radiolabeled with technetium-99m (99mTc) by stannous reduction method. Labeling conditions were optimised to achieve
high-labeling efficiency, in vitro and in vivo (serum) stability. The labeled complexes also showed very low transchelation as
determined by DTPA challenge test. Biodistribution studies of 99mTc-labeled complexes were performed after intravenous
administration in mice. The CHL-loaded PLGA NPs coated with PS-80 exhibited relatively high brain uptake with
comparatively low accumulation in bone marrow to that of free drug and CHL-loaded PLGA NPs (PVA, used as emulsion
stabilizer) at 24 h post injection time period. This indicates the usefulness of the above delivery system for prolonged use of the
antibiotic
Development and Physical Characterization of Chloramphenicol Loaded Biodegradable Nanoparticles for Prolonged Release
The objectives of our study were to prepare a biodegradable nanoparticulate system of chloramphenicol (CHL) and to evaluate its ability to prolong in vitro release of CHL compared to free drug suspension (FDS). CHL-loaded polylactide-co-glycolide nanoparticles (CHL-PLGA-NPs) were prepared by an emulsion/solvent evaporation method using ethyl acetate and polyvinyl alcohol. CHL-PLGA-NPs were
characterized by particle size, zeta potential, infrared spectra, drug entrapment efficiency and in vitro
release kinetics measurement. Sonication was done with an ultrasound pulse sonicator at 70 W, 30 kHz for 60 s to produce stable NPs of mean size range from 277 nm to 433 nm. Drug to polymer ratio (D : P) was selected as formulation variable and significantly influenced entrapment efficiency (�30% to 66%) and release (p < 0.05). Entrapment of CHL in biodegradable NPs significantly prolonged drug release compared to FDS and thus implies potential antibiotic delivery system for ocular application