SEASONAL VARIATION IN THE CONTENT OF MANGIFERIN IN LEAVES OF MANGIFERA INDICA L.

Objective: Mangifera indica L., a member of the genus of evergreen trees, is cultivated extensively in India. The various parts of the tree find its use in the Ayurvedic system of medicine against diseases like diarrhoea, dysentery, dyspepsia, sprue and carbuncle. The major constituent of the stem bark and leaves of M. indica is mangiferin and the various activities associated with the stem bark or the leaves are reported to be due to this component. Mangiferin is currently of interest due to its activities like antidiabetic, hypolipidemic, gastroprotective, antibacterial, chemopreventive and many more. The objective of the current study was to evaluate the variation in the mangiferin content with time, in leaves of M. indica, throughout the year.Methods: Samples of M. indica leaves were collected every month for a period of one year and the content of mangiferin was assessed at these time points. Mangiferin was quantified using the HPLC method.Results: Seasonal variation was seen in the mangiferin content of the leaves. Highest content was seen during the month of April.Conclusions: Content of mangiferin changes with the different seasons and reaches its highest value during fruit ripening.Â

Fate of β-asarone in Ayurvedic Sodhana process of Vacha

Background: Calamus (Acorus calamus Linn., Araceae) rhizome synonymously called sweet flag or Vacha is an aromatic herb indigenous to Central Asia and Eastern Europe. It has been used by the Ayurvedic practitioners since time immemorial for diseases ranging from weakness of memory to being used as an anthelminthic. Reports of its use have been found in books like Charak Samhita, Sushruta Samhita, etc., The major constituent of the oil of Vacha is a phenyl propanoid called β-asarone, which is reported to show carcinogenic properties. Due to the toxic effects of β-asarone, sodhana prakriya (detoxification process) has been prescribed for Vacha before its inclusion in the Ayurvedic medicines. Shodhanaprakriya (S.prakriya) of Vacha has been mentioned in the Ayurvedic texts. Objectives: This study was undertaken with an aim to find out the mechanism involved in the S. prakriya of Vacha and also to suggest an alternate method for the conventional one.Materials and Methods: The conventional method was studied in the laboratory and equivalent alternate methods were designed based on the mechanism involved. Vacha samples were subjected to the conventional method as well as the alternate methods and the content of β-asarone in the different samples was monitored using Gas Chromatography technique. Results: Various alternate methods have been devised based on the mechanism involved in theS. prakriya which have given results comparable with those of the conventional method. Conclusion: The scientific mechanism involved in the S.prakriya of Vacha has been established and alternate methods have been proposed