Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids

A model immunoassay test system is proposed based on metal enhanced photoluminescence to analyze low concentrations of alpha-fetoprotein, a tumor marker. Antigen-antibody reaction was performed on polystyrene plates coated with silver nanoparticles to increase sensitivity of fluorescent immunoassay and signal-to-noise ratio as compared to silver-free system. The proposed test system model uses layer-by-layer assembly approach, LED excitation and nanowatt photodetection set-up, so it is characterized by smaller probe volume, fast analysis and simplicity. The proposed model system offers alpha-fetoprotein detection at concentrations used in clinical practice. Photostability and photoluminescence enhancement for labeled alpha-fetoprotein antibodies on a silver substrate were found to depend on antibodies concentration. Labeled antibodies photostability on substrates was found to deviate within 20% as compared to silver-free samples whereas photoluminescence intensity enhancement is typically in the range of 6-8 times.Published versio

Non-resonant enhanced Raman scattering of light by ZnO nanocrystals near silver and gold colloidal nanoparticles

International audienc

Non-resonant enhanced Raman scattering of light by ZnO nanocrystals near silver and gold colloidal nanoparticles

International audienc

Nonresonant Surface-Enhanced Raman Scattering of ZnO Quantum Dots with Au and Ag Nanoparticles

International audiencePronounced 104-fold enhancement of Raman scattering has been obtained for ZnO nanocrystals on substrates coated with 50 nm Ag nanoparticles under nonresonant excitation with a commercial red-emitting laser. This makes feasible beyond 10–18 mole detection of ZnO nanocrystals with a commercial setup using a 0.1 mW continuous wave laser and can be purposefully used in analytical applications where conjugated nanocrystals serve as Raman markers. For Au-coated surfaces the enhancement is much lower and the heating effects in the course of Raman experiments are pronounced

”Green” economy: from global concept to reality of local development

The publication was carried out on the initiative and assistance of the Project “Green Decisions of Business - Unity for Sustainable Development”, which is implemented by the Dnipropetrovsk Regional Organization of Employers, in partnership with the Dnipropetrovsk Regional Council, the Dnipropetrovsk Investment Agency with the support of the Program for the Promotion of Green Modernization of the Ukrainian Economy, implemented by the German Society for International Cooperation (GIZ) GmbH. The monograph is devoted to the study of various aspects of the development of the "green" economy both globally and locally. The theoretical foundations of the development of the "green" economy, the features of the functioning of the markets for "green" goods and services, the world experience in the development of the "green" economy, the prospects for the development of the "green" economy at the local and regional level are substantiated. The collective monograph will be interesting for the general public: students, teachers, scientists, businessmen, officials, government officials