Challenging the growing rabbit with a moderately pathogenic E. coli under ad libitum or limited feed intake conditions: impact on digestive physiology, bacterial communities, and on post-weaning growth

[EN] The impact of a challenge with moderately pathogenic Escherichia coli O128:C6 on the digestive physiology and gut bacterial community of growing rabbits under two feeding programmes was analysed. Upon weaning (28 d old), 180 rabbits were allocated to four groups (9 cages of 5 rabbits per group) for two weeks: group C100 was non-inoculated and fed ad libitum; C70 was non-inoculated and feed intake was limited to 70% of C100; I100 and I70 were inoculated and fed ad libitum or restricted to 70%, respectively. At the age of 31 d (D0), rabbits were orally inoculated with E. coli (2.2×108 colony forming units/rabbit). The effects of inoculation spiked on D4, with a 28% lower growth rate for I100 than for C100. Limited feed intake reinforced the inoculation’s effects on growth: I70 had a 66% lower growth rate than C70. The morbidity rate peaked at 42% between D4 and D7 for inoculated groups, without significant effect of the feed intake level. E. coli concentration peaked on D5/D6 in the caecum of the I100 and I70 groups. Inoculation reduced by 30% (P<0.05) the villus height/crypt depth and villus/crypt area ratios in the ileum, with no significant effect of the intake level. Inoculation was associated with a tenfold increase in serum haptoglobin (P<0.001) for both ad libitum and restricted rabbits. On D5, the inoculation modified the structure of the ileal bacterial community (P<0.05), but not that of the caecum. The feed intake level did not affect either the structure or diversity of the bacterial community, both in the ileum and caecum.The authors would like to thank Alain Milon and Stéphane Bertagnoli (ENV Toulouse) who provided the E. coli O128:C6 strain we used. We are also grateful to ANSES staff in the “Service d’Elevage et d’Expérimentation en Pathologie Aviaire” (M. Amelot, L. Le Moal, T. Le Coq, D. Courtois and M. Morvan) and for the technical help of F. Lalande (HQPAP, ANSES) and L. Gordon.Martignon, M.; Burel, C.; Licois, D.; Reperant, E.; Postollec, G.; Valat, C.; Gidenne, TN. (2021). Challenging the growing rabbit with a moderately pathogenic E. coli under ad libitum or limited feed intake conditions: impact on digestive physiology, bacterial communities, and on post-weaning growth. World Rabbit Science. 29(1):1-10. https://doi.org/10.4995/wrs.2021.14089OJS110291Agnoletti F. 2012. Update on rabbit enteric diseases: despite improved diagnostic capacity, where does disease control and prevention stand? In: Proc. 10th World Rabbit Congress, World Rabbit Science Association (WRSA) publ., Sharm El Sheik, Egypt, 1113-1127.Allison S.D., Martiny J.B.H. 2008. Resistance, resilience, and redundancy in microbial communities. Proc. Nat. 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Network information and connected correlations

Entropy and information provide natural measures of correlation among elements in a network. We construct here the information theoretic analog of connected correlation functions: irreducible $N$--point correlation is measured by a decrease in entropy for the joint distribution of $N$ variables relative to the maximum entropy allowed by all the observed $N-1$ variable distributions. We calculate the ``connected information'' terms for several examples, and show that it also enables the decomposition of the information that is carried by a population of elements about an outside source.Comment: 4 pages, 3 figure

In vitro Validation of Quantitative Light-Induced Fluorescence for the Diagnosis of Enamel Fluorosis in Permanent Teeth

This study aimed to validate quantitative light-induced fluorescence (QLF) as a diagnostic tool for mild and moderate enamel fluorosis in permanent teeth, comparing it to visual diagnosis and histological assessment completed using polarized light microscopy (PLM). The buccal surfaces of 139 teeth were visually classified using the Thylstrup and Fejerskov Index (TFI) into sound (TFI 0; n = 17), mild (TFI 1-2; n = 69), and moderate (TFI 3-4; n = 43) fluorosis. Fluorosis was then assessed with QLF (variables ΔF, A, and ΔQ at 5-, 15-, and 30-radiance thresholds) using as reference areas the entire surface and a region of interest (ROI), identified as the most representative region of a fluorosis lesion. PLM images of longitudinal thin sections including the ROI were assessed for histological changes. Correlations among TFI, PLM, and QLF were determined. A receiver-operating characteristic curve was conducted to determine QLF's diagnostic accuracy when compared to the TFI and PLM assessments. This was used to assess the probability that the images were correctly ranked according to severity as determined by PLM and TFI. A positive correlation was found between QLF and PLM, and between QLF and TFI. QLF showed the highest sensitivity and specificity for the diagnosis of mild fluorosis. There was also a strong agreement between TFI and PLM. The selection of a ROI resulted in a stronger correlation with TFI and PLM than when the entire surface was used. The study results indicate that defining an ROI for QLF assessments is a valid method for the diagnosis of mild and moderate enamel fluorosis

Update of Laparoscopic Surgery in Borderline Ovarian Tumor: Systematic Review

Background: Borderline ovarian tumor (BOT) predominantly affects young women and is often diagnosed at an early stage. BOT accounts for 15% of all epithelial tumors. In this regard, a minimally invasive surgical approach and the ability to preserve fertility, without increasing the incidence of recurrences or worsening the prognosis, are crucial. This review aims to provide an update on the role and indications of laparoscopic surgery in BOTs. Methods: The electronic research was performed on Pubmed, Medline, and Embase. Articles published in the last 20 years (2004–2023) were included, and the following keywords were used: ‘borderline ovarian tumor’ and ‘laparoscopic surgery’, ‘borderline ovarian tumor’ and ‘minimally invasive surgery’, ‘borderline ovarian tumor’ and ‘fertility sparing’, ‘borderline ovarian tumor’ and ‘recurrence’ and ‘Borderline ovarian tumor’ and ‘relapse’. The agreement about potential relevance was reached by consensus of the researchers and according to PRISMA statement guidelines. We thoroughly reviewed all bibliographies to assess the inclusion of any further eligible studies. We excluded studies that did not align with the study’s objectives. Results: The electronic database search yielded 767 total studies. Of whom, 188 were published before 2004, 84 were case reports, and 45 were not in the English language. Of the remaining 450 studies, 148 were considered eligible for the study. We included 20 studies in this review. Conclusions: Despite the latest guidelines recommending an open approach for the treatment of BOT, the laparoscopic approach has gained popularity as a feasible and safe alternative. The use of an endo-bag, along with advanced laparoscopic skills, has made the minimally invasive approach increasingly safe, with oncological outcomes almost comparable to those of reference. Moreover, in the context of fertility-sparing surgery (FSS), laparoscopy seems to be associated with improved obstetrical outcomes, without detrimental effects on overall survival and disease-free survival. Therefore, the laparoscopic approach in the treatment of BOT appears to be a safe and effective option, especially in the case of FSS

CariesCare practice guide : consensus on evidence into practice

This CariesCare practice guide is derived from the International Caries Classification and Management System (ICCMS) and provides a structured update for dentists to help them deliver optimal caries care and outcomes for their patients. This '4D cycle' is a practice-building format, which both prevents and controls caries and can engage patients as long-term health partners with their practice. CariesCare International (CCI™) promotes a patient-centred, risk-based approach to caries management designed for dental practice. This comprises a health outcomes-focused system that aims to maintain oral health and preserve tooth structure in the long-term. It guides the dental team through a four-step process (4D system), leading to personalised interventions: 1st D: Determine Caries Risk; 2nd D: Detect lesions, stage their severity and assess their activity status; 3rd D: Decide on the most appropriate care plan for the specific patient at that time; and then, finally, 4th D: Do the preventive and tooth-preserving care which is needed (including risk-appropriate preventive care; control of initial non-cavitated lesions; and conservative restorative treatment of deep dentinal and cavitated caries lesions). CariesCare International has designed this practice-friendly consensus guide to summarise best practice as informed by the best available evidence. Following the guide should also increase patient satisfaction, involvement, wellbeing and value, by being less invasive and more health-focused. For the dentist it should also provide benefits at the professional and practice levels including improved medico-legal protection

Weak pairwise correlations imply strongly correlated network states in a neural population

Biological networks have so many possible states that exhaustive sampling is impossible. Successful analysis thus depends on simplifying hypotheses, but experiments on many systems hint that complicated, higher order interactions among large groups of elements play an important role. In the vertebrate retina, we show that weak correlations between pairs of neurons coexist with strongly collective behavior in the responses of ten or more neurons. Surprisingly, we find that this collective behavior is described quantitatively by models that capture the observed pairwise correlations but assume no higher order interactions. These maximum entropy models are equivalent to Ising models, and predict that larger networks are completely dominated by correlation effects. This suggests that the neural code has associative or error-correcting properties, and we provide preliminary evidence for such behavior. As a first test for the generality of these ideas, we show that similar results are obtained from networks of cultured cortical neurons.Comment: Full account of work presented at the conference on Computational and Systems Neuroscience (COSYNE), 17-20 March 2005, in Salt Lake City, Utah (http://cosyne.org

Non-invasive study of natural dyes on historical textiles from the collection of Michelangelo Guggenheim

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