Arum macalatum bitkisinin yara iyileştirici aktivitesi

Objective: In this study, the antioxidant properties of Arum maculatum plant were evaluated. This study reported for the first time the wound healing activity of the methanol extract of A. maculatum fruits. This study aimed to assess and determine the possible pharmacological activities of A. maculatum and evaluate its potential to act as a wound care plant. Methods: The antioxidant and antimicrobial activities of A. maculatum were investigated using excisional in vivo and in vitro wound healing mouse models. A total of 32 Balb-c mice were used, which were equally, divided into four groups: saline control group, control group, A. maculatum group, and Centella asiatica extract group. Treatment applications were performed topically once per day. Wound area narrowing, wound healing percentage, and epithelialization time were analyzed. Results: A. maculatum application supported the healing process in in vivo and in vitro wound models. A. maculatum contributed to the healing process by promoting granulation tissue formation, epidermal regeneration, and angiogenesis. Conclusions: Wound healing is a complex and well-organized process that requires communication between cells. The antioxidant and antimicrobial activities of A. maculatum extract have been determined by current studies. A. maculatum extract may provide significant benefits in promoting the wound healing process.Amaç: Bu araştırmada antioksidan özelliklerini değerlendirmek için Arum maculatum bitkisi seçilmiştir. Bildiğimiz kadarıyla A. maculatum meyvelerinin metanol özünün yara iyileştirici aktivitesi ilk kez bu çalışmada rapor edilmiştir. Bu çalışma, A. maculatum’un olası farmakolojik aktivitelerini belirlemek, değerlendirmek ve bir yara tedavi edici bitki olarak etki gösterme potansiyelini değerlendirmek içindi. Yöntemler: A. maculatum’un antioksidan ve antimikrobiyal aktiviteleri, farelerde eksizyonel in vivo ve in vitro yara iyileşme modelleri kulanılarak araştırılmıştır. Toplamda 32 Balb-c fare kullanılmış olup salin kontrol grubu, kontrol grubu, A. maculatum uygulanan grup ve Centella asiatica özütü uygulanan grup olmak üzere 4 gruba ayrılmıştır. Tedavi uygulamaları günde bir kez topikal olarak gerçekleştirilmiştir. Skar alanı hacminde gerçekleşen değişim, yara iyileşme yüzdesi ve epitelizasyon süresi analiz edilmiştir. Bulgular: A. maculatum uygulaması in vivo ve in vitro yara modelinde iyileşme sürecini desteklemiştir. A. maculatum, granülasyon dokusunu artırarak iyileşme sürecine katkıda bulunmuş, epidermal rejenerasyonu ve anjiyogenezi artırmıştır. Sonuçlar: Yara iyileşmesi, hücreler arası iletişimi gerektiren karmaşık ve iyi organize edilmiş bir süreçtir. Mevcut çalışmalar doğrultusunda antioksidan ve antimikrobiyal aktivitesi belirlenmiş olan A. maculatum özü, yara iyileşme sürecinin desteklenmesinde önemli bir fayda sağlayabilir

The effects of topical carvacrol application on wound healing process in male rats

Carvacrol containing products such as Origanum onites have been used as phytotherapeutic agents in the treatment of serious skin injury. It has been suggested that carvacrol is the active component of these herbs because of its anti-microbial property. With the anti-microbial activity and as an ingredient of these herbs, carvacrol is a promising molecule for the treatment of skin injury. In the present study, we have evaluated the efficacy of carvacrol on healing progress after excisional skin injury. Here, Wistar-Albino rats were divided into two groups and treated with carvacrol and vehicle. Carvacrol was administrated topically at a concentration of %12.5 for the 5 consecutive days after excisional skin injury. Tissue samples were harvested on days 3rd, 8th and 12th after injury. Significant beneficial effect of carvacrol was observed at the end of the experiment. In the acute phase of the injury, carvacrol treatment increased tissue granulation and decreased wound depth moderately. These effect of carvacrol was associated with increased TNF-?. However, at the second half of the experiment the elevated level of TGF-B1 was observed as compared with control animals. The level of IL-1ß was increased in carvacrol treated animals only on day 8. Here, we provide evidence that carvacrol improves wound healing by regulating pro- inflammatory molecules TNF-?, IL-1ß and TGF-ß1

Potential effect of 2-isopropyl-5-methylphenol (Thymol) alone and in combination with selenium on apoptosis, intracellular calcium, caspase 3 and 9 levels through activation of TRPV1 channel

3rd International Congress of the Turkish-Neuroendocrinology-Society -- JUN 29-JUL 01, 2018 -- Malatya, TURKEYWOS: 000445952400020…Turkish Neuroendocrinol Societ

Experimental data of labeling the heart and cardiac cultures with a retrograde tracer in vitro and in vivo

Retrograde dyes are often used in basic research to investigate neuronal innervations of an organ. This article describes the experimental data on the application of retrograde dyes on the mouse heart in vivo and on the cardiac or neuronal cultures in vitro. By providing this information, cardiac or inneinnervations can be evaluated in vivo. Therefore, unknown cellular and molecular mechanisms and systemic interactions in the body can be investigated. In particular, we provided practical tips to lower mortality risks following the cardiac surgery and evaluated the staining capacity and fluorescent characteristics of the Di-8-ANEPPQ dye in the cardiac tissue and cell cultures. First, primary cultures of mouse nodose ganglia (NG) neurons and mouse neonatal cardiomyocytes were stained with Di-8-ANEPPQ. The Di-8-ANEPPQ signal from live cultures were visualized using spinning disk confocal microscopy to verify the lipophilic and fluorescent labeling capacity of Di-8-ANEPPQ. Next, the excitation and emission data of Di-8-ANEPPQ were collected between 415 nm and 690 nm using power spectrum module of confocal microscopy. This spectrum analysis could be useful for the researchers who plan to use Di-8-ANEPPQ in combination with other fluorescent dyes to eliminate any florescent overlap. In order to label the heart tissue with tracer dyes Di-8-ANEPPQ or DiI in vivo, the heart was exposed without damaging lungs or other tissues following anesthetization, then the retrograde dye was applied as a paste for DiI or injected to the apex of the heart for Di-8-ANEPPQ and the operation area was sutured. The surgical procedure required intubation to control the respiratory reflex without the need to perform a tracheotomy and yielded high viability. Following labeling the heart in vivo, the heart was dissected, and images of injection area were captured using confocal microscopy. All fluorescent images of Di-8-ANEPPQ labeled cells were analyzed by using the Fiji software. Overall, these data provide applicable data to other investigators to trace the sensory neurons innervating not only the heart but also other organs using Di-8-ANEPPQ. These data support the original research article titled “Evaluation of bilateral cardiac afferent distribution at the spinal and vagal ganglia by retrograde labeling” that was accepted for publication in Brain Research Journal [1]

The effect of probiotic and omega-3 supplements on total oxidant and total antioxidant levels in experimental colitis

Objective: Treatment of inflammatory bowel disease (IBD) usually involves medical therapy. For this reason, it is recommended to seek alternative treatment methods such as nutritional therapy. The aim of the study is to evaluate the effects of probiotics and omega-3 fatty acids on total oxidant and total antioxidant levels in an experimental colitis model. Methods: Mice were randomly divided into five groups (n= 10/group) as healthy group, colitis group, group treated with probiotics (VSL#3), group treated with omega-3 (w-3), and group treated with both probiotics and omega-3. To induce experimental colitis, 200 mg/kg dinitrobenzene sulfonic acid (DNBS) + 30% ethanol combination was rectally administered to anesthetized mice. Total oxidant (TOS) and total antioxidant (TAS) levels were measured at the tissue level. Results: Lower concentrations of TOS were observed in the probiotics groups (2.11 +/- 0.23 mmol H2O2 Eq/L), probiotics+omega-3 (2.56 +/- 1.18 mmol H2O2 Eq/L), and omega-3 (3.02 +/- 1.88 mmol H2O2 Eq/L) groups compared to the colitis group (3.11 +/- 0.91 mmol H2O2 Eq/L) (p>0.05). Higher TOS and TAS level were observed in the control colitis group compared to other groups; however, the differences were not statistically significant. Conclusion: Our findings showed that TAS and TOS levels were positively affected by the use of probiotic supplements in IBD. It was determined that using w-3 alone was ineffective in decreasing TOS levels. Studies with higher dosages and longer treatment periods are needed to better observe the effects of nutritional supplements on TOS and TAS parameters in inflammatory bowel diseases

Role of erythropoietin and its receptor in the development of endometriosis in rats

WOS: 000459590500009PubMed ID: 29916217Objective: Besides its hematopoietic function, erythropoietin (EPO) may protect tissues from degenerative disorders. As such, EPO and its receptors were revealed in nonhematopoietic cells, including stromal and endometrial epithelial cells. However, the role of EPO in endometrial disorders is still unknown. Here, we aimed to examine the role of EPO and its receptor activation in the development of endometriosis in rats. Material and Methods: Animals were treated with EPO, darbepoietin (the synthetic form of EPO) or EPO's receptor activator, methoxy polyethylene glycol-epoetin beta (MIRCERA), after development of endometriosis. Endometriosis was induced by estrogen-administration following surgical attachment of endometrial surface on the inner abdominal wall. Treatments were started 3 weeks after induction of endometriosis and continued for the following 3 weeks. For the analysis of recurrence of endometriosis, additional analyses were conducted 3 weeks after cessation of treatments. Results: As compared with vehicle-treated animals, lesion size was reduced significantly and recurrence of endometriosis was not observed in all treatment groups. Histopathologic examination revealed that EPO and darbepoietin were more effective than MIRCERA- and vehicle-treated animals. Conclusion: Here we provide evidence that EPO is a promising candidate for the treatment of endometriosis. Our histopathologic results in particular indicate that EPO is more effective than its receptor activator MIRCERA in the development endometriosis.Turkish Academy of Sciences (TUBA)This work was funded by Turkish Academy of Sciences (TUBA). We would like to thank to Laboratory Technician Nurhayat Donek from Bezmialem University for help throughout this research

Jiný pohled na inhibitor agregace krevních destiček klopidogrel – vhodné antiedematikum v předklinickém modelu poranění mozku?

Aim: The neuroprotective effects of clopidogrel have already been shown in various experimental models. Taking into account the fact that clopidogrel is well tolerated and approved for use in various clinical settings, it can be an attractive candidate for further clinical investigations, especially when the anti-oedema effect appears to be a reasonable adjuvant strategy, such as in brain injury (BI). Here we aimed to examine the neuroprotective role of clopidogrel in BI. Methods: To investigate the effects of clopidogrel, we induced BI in mice using a cold trauma model and evaluated the underlying cell survival/death mechanisms via cresyl violet, TUNEL staining and western blot analysis. Results: Clopidogrel at a dose of 3 mg/kg led to a significant reduction in brain swelling. Similar decreases were observed with 10 mg/kg and 30 mg/kg of clopidogrel. We also have shown that clopidogrel blocks the prominent inflammatory injury pathways and exerts a significant anti-apoptotic effect (3 and 30 mg/kg), which has boon associated with increased neuronal cell survival pathways. Clopidogrel (3, 10 and 30 mg/kg) dose-dependently altered the JNK, p-38, AKT, ERK and p53 levels. Conclusion: Our findings demonstrate that clopidogrel can be a novel candidate for the reduction of post-traumatic BI and oedema. We propose that it can be applied mainly in the acute phases of cerebral ischaemia, which is characterized by haemorrhagic transformation and brain oedema.Cíl: Neuroprotektivní účinky klopidogrelu již byly prokázány na různých experimentálních modelech. Vzhledem k tomu, že klopidogrel je dobře tolerován a schválen k používání v různých klinických podmínkách, může být atraktivním kandidátem pro další klinická zkoumání, zejména v situaci, kdy se antiedematický účinek jeví jako vhodná adjuvantní strategie, například při poranění mozku. Naším záměrem bylo prozkoumat neuroprotektivní roli klopidogrelu při poranění mozku. Metody: Pro zkoumání účinků klopidogrelu jsme navodili poranění mozku s použitím modelu chladového traumatu u myší a zkoumali jsme základní mechanizmus přežití/odumírání buněk s použitím kresolové violeti, barvení TUNEL a analýzy western blot. Výsledky: Klopidogrel v dávce 3 mg/kg vedl k signifi kantnímu snížení otoku mozku. Podobný pokles byl pozorován při dávce klopidogrelu 10 mg/kg a 30 mg/kg. Rovněž jsme prokázali, že klopidogrel blokoval prominentní cesty poškození zánětem a vykazoval silný antiaptotický účinek (3 a 30 mg/kg), který byl spojen se zvýšenou možností přežití neuronových buněk. Klopidogrel (3, 10 a 30 mg/kg) měnil dávkově dependentním způsobem hladiny JNK, p-38, AKT, ERK a p53. Závěr: Naše nálezy prokazují, že klopidogrel může být novým kandidátem pro zmírnění posttraumatického poranění mozku a edému. Domníváme se, že může být aplikován hlavně v akutních fázích cerebrální ischemie, která je charakterizována hemoragickou transformací a edémem mozku

Squalene attenuates the oxidative stress and activates AKT/mTOR pathway against cisplatin-induced kidney damage in mice

WOS: 000471268100003The clinical use of cisplatin, which is a first-line anticancer agent, is highly restricted due to its adverse effects on kidneys that lead to nephrotoxicity. Therefore, some potential reno-protective substances have been used in combination with cisplatin to cope with nephrotoxicity. Due to its high antitumor activity and oxygen-carrying capacity, we investigated the molecular effects of squalene against cisplatin-induced oxidative stress and kidney damage in mice. Single dose of cisplatin (7 mg/kg) was given to male Balb/c mice. Squalene (100 mg/kg/day) was administered orogastrically to mice for 10 days. Following sacrification, molecular alterations were investigated as analysis of the levels of oxidative stress index (OSI), inflammatory cytokines and cell survival-related proteins in addition to histopathological examinations in mice kidney tissue. The level OSI and Interferon-gamma (IFN-gamma) decreased in the cisplatin and squalene cotreated mice compared to cisplatin-treated mice. Squalene treatment also increased the activation of protein kinase B (AKT). Furthermore, cisplatin-induced inactivation of mammalian target of rapamycin (mTOR) and histopathological damages were reversed by squalene. It may be suggested that squalene ameliorated the cisplatin-induced histopathological damages in the kidney through activation of AKT/mTOR signaling pathway by regulating the balance of the redox system due to its antioxidative effect

Sodium pentaborate pentahydrate and pluronic containing hydrogel increases cutaneous wound healing in vitro and in vivo

After a disruption of skin integrity, the body produces an immediate response followed by a functional and comparable regeneration period, referred to as wound healing. Although normal wounds do not need much attention during the healing period, chronic (non-healing) wounds are the major challenge of current dermatological applications. Therefore, developing new, safe, and effective wound healing drugs has always been an attractive area of international research. In the current study, sodium pentaborate pentahydrate (NaB), pluronics (Plu; F68 and F127), and their combinations were investigated for their wound healing activities, using in vitro and in vivo approaches. The results revealed that NaB significantly increased migration capacity and superoxide dismutase activity in primary human fibroblasts. Combinations of optimized concentrations for pluronic block co-polymers further increased cell migration, and the messenger RNA (mRNA) expression levels of important growth factor and cytokines (vascular endothelial growth factor (VEGF), transforming growth factor beta (TGF-ß), and tumor necrosis factor alpha (TNF-?)). NaB containing hydrogel co-formulated with pluronics was also investigated for their wound healing activities using a full thickness wound model in rats. Macroscopic and histopathological analysis confirmed that wounds in combination gel-treated groups healed faster than those of control groups. NaB/Plu gel application was found to increase wound contraction and collagen deposition in the wound area. Therefore, our results suggest that NaB, and its pluronics combination, could be used in dermatological clinics and be a future solution for chronic wounds. However, further studies should be conducted to explore its exact action of mechanism and effects of this formulation on chronic wounds

Polypodium vulgare ile yara iyileşme alanına alternatif bir yaklaşım

Objective: In this study, we examined the effects of Polypodium vulgare L. (Polypodiaceae) as a candidate to be used for wound healing scarred area. We investigated the antibacterial, and antioxidant activity of P. Vulgare on both in vivo, and in vitro wound healing using an excisional wound model in mice.Method: We used 32 Balb-c mice equally divided into four groups: Group 1 control, Group 2 vehicle, Group 3 Polypodium vulgare, and Group 4 Centella asiatica extract (CAE). All treatments were applied topically once in a day. The scar area, percentage wound closure and epithelization time were measured. PDGF, VEGF, and collagen immunohistochemical staining were used for evaluation.Results: CAE and P. vulgare extract groups were observed to be more effective than the control and vehicle groups in terms of new vascular, epidermal and granulation tissue organization. PDGF, VEGF, and collagen immunohistochemical staining was stronger in the P.vulgare extract and CAE groups compared to the control and vehicle groups. In the P. vulgare and CAE groups, PDGF staining intensity was stronger than the control and vehicle groups, but VEGF and collagen staining in P. vulgare group was not different from the control group.Conclusion: P. vulgare had an effect on the injured area by regenerating the epidermis and increasing vascularization. P. vulgare extract with known antioxidant, and antimicrobial activities may be helpful as a supportive treatment in wound healing.Amaç: Bu çalışmada Polypodium vulgare L. (Polypodiaceae), yara iyileşme adayı olma potansi yeli açısından değerlendirildi. Farelerde eksizyonel bir yara modeli kullanılarak hem in vivo hem de in vitro yara iyileşmesinde P.vulgare’nin antimikrobiyal ve antioksidan aktivitesini araştırdık. Yöntem: Eşit olarak dört gruba ayrılmış toplam 32 Balb-c faresi kullandık; Grup 1 kontrol, grup 2 taşıyıcı grup 3 Polipodyum vulgare, grup 4 Centella asiatica özütü (CAE). Tüm tedaviler topikal olarak günde bir kez uygulandı. Yara izi alanı, yara kapanma yüzdesi ve epitelizasyon süresi ölçül dü. İmmünhistokimyasal değerlendirmede PDGF, VEGF ve kollajen boyaları kullanıldı. Bulgular: C A E v e P. v u l g a r e e k s t r a k t g r u p l a r ı n ı n y e n i v a s k ü l e r o r g a n i z a s y o n , e p i d e r m i s v e granülasyon doku organizasyonu açısından kontrol ve taşıyıcı gruplarından daha etkili olduğu gözlenmiştir. PDGF, VEGF ve kollajen immünohistokimyasal boyama, P.vulgare ekstraktı ve CAE gruplarında kontrol ve taşıyıcı gruplarına göre daha kuvvetliydi. P. vulgare ve CAE grubunda PDGF boyama yoğunluğu, kontrol ve taşıyıcı gruplarından daha kuvvetliydi, ancak P. vulgare grubundaki VEGF ve kollajen boyama, kontrol grubundan farklı değildi. Sonuç: P. vulgare yara iyileşmesini ve granülasyon dokusunu, epidermal rejenerasyonu ve anjiyogenezi arttırdı. Antioksidan ve antimikrobiyal aktiviteleri ile bilinen P. vulgare ekstraktı, yara iyileşmesini desteklemek için yararlı olabilir