DETHEMO. Teilprojekt 4: Untersuchungen zur antiviralen Wirksamkeit von de novo-designten Peptid- und RNA-Molekuelen Schlussbericht

One of the major causes of common cold, human rhinovirus 14 (HRV14), and its receptor, intercellular adhesion molecule-1 (ICAM-1), were used as a model for the development of antiviral compounds. Aim of the antiviral intervention was the cell protection against HRV14 infections. Peptide sequences for the blockade of the virus receptor interaction were derived from the 3D-structures and amino acid sequences of HRV14 and ICAM-1. The peptides were synthesized by the project partner WITA GmbH. The antiviral efficacy of the de novo designed peptides and high affinity RNA molecules (haRNAs) selected by in vitro evolution techniques were measured as inhibition of HRV14 reproduction in in vivo test systems. The sequence properties of the peptides and their antiviral effect were analyzed and optimized with new algorithms developed and applied by the bioinformatic working groups of the joint project. 253 peptides in total were characterized in the virology group. 38 of the 253 peptides showed a significant inhibition of HRV14 reproduction of more than -30%. One peptide resulted in an inhibition of virus reproduction of -74%. The best of the selected haRNA molecules exhibited an inhibition of HRV14 reproduction of up to -53%. (orig.)SIGLEAvailable from TIB Hannover: F99B1324+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Transgene Ratten als Modell zur Untersuchung der potenzierenden Effekte von Umweltstoffen mit oestrogener Wirkung Abschlussbericht

Available from TIB Hannover: F04B807 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung und Forschung (BMBF), Bonn (Germany)DEGerman

Entwicklung effizienter Verpackungssysteme fuer AAV-Vektoren Schlussbericht

SIGLEAvailable from TIB Hannover: DtF QN1(93,6) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung und Forschung, Berlin (Germany)DEGerman

HTS-SQUID-Systeme fuer die Kardiologie. Erstellung eines Referenz-Datensatzes der Mehrkanal-LTS-SQUID-Magnetokardiographie und Vergleich mit Befunden der HTS-SQUID-Magnetokardiographie Abschlussbericht

SIGLEAvailable from TIB Hannover: F99B29+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Laserforschung. Grundlagenuntersuchungen zur Wirkung der kombinierten Anwendung von NIR-Laserstrahlung und Hochfrequenzstrom Abschlussbericht

fState of research: Biological tissue is cut and coagulated by NIR-lasers and monopolar RF (radio frequency)-current. Bipolar RF-cutters are occasionally available. Bipolar interstitial coagulation has not been performed yet. The possibilities of the combination of both types of energy has not been investigated yet too. Reason for / aim of investigation: It has to be investigated if an improvement of surgical procedures (cutting, coagulation, interstitial coagulation) is possible by the combination of laser radiation and RF-current. Method: Construction of bipolar RF-cutting applicators and bip. RF-ITT (interstitial thermotherapy) probes (bringgn the RF-ITT to the same level of development as the LITT). Construction of combined instruments (cutting with RF, coagulating with the laser). In-vitro-investigation of the effect on biological tissue. Development of a computer model for the simulation of the interstitial application of laser radiation and RF current. Results: The bipolar RF-cutter and the RFITT probe respectivley, have proven to be a powerful alternative to the corresponding laser techniques (bare fiber / focusing hand piece, LITT). The combination of laser radiation and RF current does only make sense in those cases when both techniques are assigned different tasks (e.g. RF for cutting and laser for coagulation in case of bleedings). If both types of energy are used for the same purpose (e.g. ITT) no improvement can be achieved in comparison to a single application. Conclusion / possible applications: Bipolar RFITT is a new kind of therapy with versatile possibilities of application in the field of ENT (concha hyperplasia), urology (benign prostate hyperplasia) and interventional radiology / surgery (liver metastases). The constructive (and financial) effort building up combined instruments is not justified in most of the cases. Especially for use in ITT an applicator build up solely in bipolar RF technique is a skillful and good value instrument. (orig.)SIGLEAvailable from TIB Hannover: F99B779+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Erythropoietin und Serum-Transferrin-Receptor-Konzentrationen vor, waehrend und nach Aufenthalt unter Mikro-g Bedingungen unter besonderer Beruecksichtigung des zentralen Venendrucks Abschlussbericht ueber die Berichtsjahre 1.2.1996 bis 30.6.1998

Blood samples were taken before, during and after the MIR'97 Mission as well as under terrestrial simulations to analyze the regulation of erythropoietin in humans under different environmental conditions. These studies could be finished successfully, meanwhile. Due to a fire onboard of the MIR station a set of serum samples of the present project were kept for a long time at the Medical Board to evaluate - eventually - whether poisonous substances were deliberated into the stations' atmosphere during this emergency case. Already during the early space missions a decreased number of erythroblastic cells were observed in humans. Therefore, we analyzed in the present project the erythropoietin production and transferrin-receptor concentration during a mid-term space mission as well as under terrestrial conditions. The terrestrial studies have shown that short-term physical exercise combined with severe hypoxia does not increase erythropoietin production whereas as the vascular endothelial growth factor (VEGF), which plays an important rule in vascularisation of the tissues in the body, showed a significant increase. Under micro-g conditions erythopoiesis was impaired due to a decreased erythopoietin production paralleled by a low serum transferrin receptor concentrations. These results are important to understand and develop efficient counter-measures for astronauts/cosmonaut as well as for hospitalized patients (de-conditioning, clinical relevance). Additional terrestrial studies have shown that changes in central venous pressure might modulate erythropoietin production and release in humans. (orig.)Available from TIB Hannover: F99B266 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany); DLR Deutsches Zentrum fuer Luft- und Raumfahrt e.V., Bonn (Germany)DEGerman

Neue Laserverfahren fuer die Endoskopie. Teilvorhaben: Untersuchungen eines diagnostischen Laser-Scan-Endoskops (LSE) Abschlussbericht

The intention and first results of a research project called DELAS (Diagnostic Endo Laser Scanner) will be presented. This paper is focused on the technology development of a scanning imaging method for endoscopy. This work is granted by the Bundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie (BMBF, FKZ 13 N 6796). The current endoscopic imaging technique is restricted to the visible spectral range. An extension of the usable wavelength range leads to new application fields of endoscopes, such as the use of fluorescence or other spectroscopic methods, e.g. to perform diagnostic investigations. The aim of the project is to investigate the feasibility of a scanning imaging technique on the basis of microsystem technology. The optical components will be realized by using lithographic methods in addition to single fibers and microscanning mirrors being applied to avoid the spectral limitations of conventional image guides. The integration of the microscanning device into an imaging setup of an endoscope is planned in a further project part. Initially, an experimental setup using conventional components has to be realized in order to simulate a situation considering the requirements of an endoscopic utilization. Using this setup the verification of the imaging method regarding potential applications and an improvement of the imaging parameters are performed. These investigations lead to specific requirements of a microscanning device for a future prototype manufactured by using silicon technologies. First results of the investigations are discussed in terms of lateral and depth resolution, image distortion, and non linear behavior of the scanning mirrors. They are compared to the results obtained by conventional microscopy and evaluated with regard to the requirements of diagnostic applications. (orig.)SIGLEAvailable from TIB Hannover: F00B625+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung und Forschung (BMBF), Bonn (Germany)DEGerman

Verbund Neurotrauma Magdeburg/Berlin: Zellulaere und systemische Sekundaerdegeneration und Kompensation nach Schaedigung des ZNS/Grundlagen und klinische Perspektiven Schlussbericht

Available from TIB Hannover: F00B433 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung und Forschung (BMBF), Bonn (Germany)DEGerman

Brain-Computer Interface (BCI) Schlussbericht

SIGLEAvailable from TIB Hannover: F02B1420 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung und Forschung, Berlin (Germany)DEGerman

Die Versorgung von Schlaganfallpatienten in Berlin Schlussbericht

SIGLEAvailable from TIB Hannover: F02B847 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung und Forschung, Berlin (Germany)DEGerman