The prophylactic effect of Acetobacter syzygii probiotic species against squamous cell carcinoma

Background. Squamous cell carcinoma is a prevalent carcinoma of the oral cavity. Recently anti-proliferative effect of probiotics has been considered and assessed against different cancers. The aim of this study was to evaluate the cytotoxicity of Acetobacter syzygii strain supernatant on KB human oral cancer cell line and KDR human epithelial normal cell line. Methods. The cytotoxicity assessments were performed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) as well as through qualitative (4',6-diamidino-2-phenylindole staining) and quantitative (flow cytometry assessments using the BD Biosciences Annexin V-FITC Kit) evaluations of apoptosis. Results. A. syzygii secretions exhibited significant cytotoxicity against KB cancer cell lines similar to cisplatin (75.7% apoptosis) while the rate of apoptosis in KDR normal cells was only 9.36%. The prophylactic effects of Lactobacillus acidophilus (PTCC 1643), as a reference bacterium, was similar to A. syzygii, indicating beneficial effects of useful bacteria on prevention of oral diseases. Conclusion. The anticancer bioactivity of A. syzygii strain secretions depends on the induction of apoptosis in KB cancer cells. However, several investigations should be conducted to precisely determine effective compounds to be used as anticancer therapeutics in the future

Serum Level of Interleukin-17 in Patients with Erosive and Non-erosive Oral Lichen Planus

Background and aims. Oral lichen planus is a relatively common chronic oral mucosal disease of unknown etiology. Regarding numerous studies on the immunologic factors involved in the etiology of lichen planus, the present study evaluated the serum interleukin-17 (IL-17) level in patients with erosive and non-erosive oral lichen planus. Materials and methods. This descriptive analytical study included 24 patients with erosive oral lichen planus (EOLP), 24 patients with non-erosive oral lichen planus (NEOLP) and 24 healthy volunteered as control. Blood samples of the subjects underwent ELISA, using special kits, to determine serum interleukin-17 levels. Data was analyzed using with descriptive statistic, chi-square test, and one-way ANOVA and Tukey post-hoc test with SPSS 16 software. Results. EOLP patients showed a high level of serum IL-17 compared with NEOLP patents and control groups (EOLP=184.16 ± 12.41 pg/mL, NEOLP=106.09±10.78 pg/mL, control=15.50 ± 4.34 pg/mL, P - 0.001). Conclusion. High level of serum IL-17 in erosive oral lichen planus patients compared to the non-erosive type and healthy individuals may be the reason for higher inflammation and atrophy in the erosive type

Influence of the Display Monitor on Observer Performance in Detection of Dental Caries

<p><strong>Background and aims.</strong> Digital imaging continues to gain acceptance in dentistry and video display used for this becomes important. The aim of this study was to assess the influence of the display monitor on observer performance on caries detection. </p><p><strong>Materials and methods.</strong> Artificial enamel lesions were created in 40 extracted teeth at random using 1/4 and 1/2 round burs. Teeth were mounted in dental stone blocks to simulate a hemi-dentition. Approximate exposures were recorded at 70 kVp using a Planmeca (Planmeca Co, Helsinki, Finland) digital imaging system. Three oral and maxillofacial radiologists rated each image on a five-point scale for the presence or absence of lesion. Radiographic images were viewed on the following monitors: (1) LG Flatron 700p (LG Electronics Co., South Korea); (2) Samsung Magicgreen (Samsung Electronics Corp., South Korea); (3) Hansol 710p (Hansol Electronics Corp., South Korea) and (4) Toshiba satellite laptop (Toshiba Computer Corp., Philippines). Examiners were allowed to magnify and adjust density and contrast of each image at will. Receiver Operating Characteristic (ROC) analysis was performed. Data was subjected to repeated measures analysis of variance and ordinal logistic regression to test for significance between variables and to determine odds ratios. </p><p><strong>Results.</strong> Mean ROC curve areas ranged from 0.8728 for the LG monitor to 0.8395 for the Samsung. Repeated measures analysis of variance showed significant differences between observers (P< 0.0001), lesion size (P< 0.0001), examiner/monitor interaction (P< 0.033) and examiner/block interaction (P< 0.013). However, no significant difference was found between monitors. </p><p><strong>Conclusion.</strong> This study suggests that observer performance is independent of the visual characteristics of the display monitor.</p&gt

Effect of Low-power Laser on Treatment of Orofacial Pain

Low-power lasers are a group of lasers with a power less than 250 mW and unlike high-power lasers they have no effect on tissue temperature; they produce light-dependent chemical reactions in tissues. These lasers have analgesic features with their ability to trigger reactions that reduce pain and inflammatory mediators. Low-power lasers can also be used instead of needles in acupuncture to decrease pain. Due to these features they have been used in the treatment of orofacial pain, including tooth hypersensitivity, post-operative flare-ups, mucositis, facial myalgia, temporomandibular joint disorders and neuralgia. In this article we review the effects of low-power lasers and their success rate in different studies. As the name implies (LASER: Light Amplification by the Stimulated Emission of Radiation), laser amplifies light by stimulated and excited radiation; in other words, it is amplification of excited light emission. Such radiation usually has some characteristic features, including mono-chromaticity, coherency, high intensity and polarity. There are various classifications for lasers based on their active material (solid, fluid and gas), wavelength, emission type and power

Efficacy of Elaeagnus angustifolia Topical Gel in the Treatment of Symptomatic Oral Lichen Planus

Background and aims. The purpose of this study was to determine efficacy of 19% Elaeagnus angustifolia (EA) topical gel in the treatment of symptomatic oral lichen planus. Materials and methods. Patients with symptomatic oral lichen planus referring to the Department of Oral Medicine, Faculty of Dentistry at Shahid Beheshti University of Medical Sciences were asked to participate in the study. Twenty-eight patients who were histopathologically diagnosed with lichen planus were divided into two groups (15 in the case and 13 in the control groups). The subjects were randomly assigned to either topical gel of EA or placebo in a double-blind manner. They were then instructed to apply the medication on dried lesions three times daily. Pain and size of the lesions were evaluated after 2 weeks. Data were analyzed by SPSS 12.0 software, using t-test, paired t-test, Fisher’s exact test and chi-square test. Results. Twenty-eight patients (m/f: 7/21) with symptomatic oral lichen planus participated in the study. Fifteen patients (m/f: 4/11) received E A gel and 13 patients (m/f: 3/10) received placebo. There was a 75% decrease in pain (33.3% in the case and 7.7% in the control groups), and a decrease of 50% in size (33.3% in the case group) and 75% only in 7.6% of the case group. Conclusion. The results suggest that 19% EA gel is efficient in the treatment of symptomatic oral lichen planus, with antiinflammatory and analgesic effects, as well