104 research outputs found
Aldehyde dehydrogenase 7A1 (ALDH7A1) attenuates reactive aldehyde and oxidative stress induced cytotoxicity.
High-yield method for isolation and culture of endothelial cells from rat coronary blood vessels suitable for analysis of intracellular calcium and nitric oxide biosynthetic pathway.
Oxaliplatin-induced neuropathy: oxidative stress as pathological mechanism. Protective effect of silibinin
EFFECTS OF RELAXIN ON VASCULAR SMOOTH MUSCLE AND ENDOTHELIAL CELLS IN NORMOTENSIVE AND HYPERTENSIVE RATS
Losartan ameliorates diabetic vascular hyper-reactivity to angiotensin II by reducing ROCK1 expression and activity
RELAXANT EFFECT OF A WATER SOLUBLE CARBON MONOXIDE-RELEASING MOLECULE (CORM-3) ON SPONTANEOUSLY HYPERTENSIVE RAT AORTAS
Different apoptotic pathways activated by oxaliplatin in primary astrocytes vs. colo-rectal cancer cells
Oxaliplatin-based chemotherapy improves the outcomes of metastatic colorectal cancer patients. Its most significant and dose-limiting side effect is the development of a neuropathic syndrome. The mechanism of the neurotoxicity is unclear. The limited knowledge about differences existing between neurotoxic and antitumor effects hinders the discovery of effective and safe adjuvant therapies. In vitro, we suggested cell-specific activation apoptotic pathways in normal nervous cells (astrocytes) vs. colon-cancer cells (HT-29). In the present research we compared the apoptotic signals evoked by oxaliplatin in astrocytes and HT-29 analyzing the intrinsic and extrinsic apoptotic pathways. In astrocytes, oxaliplatin induced a mitochondrial derangement measured as cytosolic release of cytochrome C, increase in superoxide anion levels and decreased expression of the antiapoptotic protein Bcl-2. Caspase-8, a main initiator of the extrinsic process remained unaltered. On the contrary, in HT-29 oxaliplatin increased caspase-8 activity and Bid expression, thus activating the extrinsic apoptosis, while the Bcl-2 increased expression blocked the mitochondrial damage. Data suggest the preferred activation of the intrinsic apoptosis as oxaliplatin damage signaling in normal nervous cells. The extrinsic pathway prevails in tumor cells indicating a possible strategy for planning new molecules to treat oxaliplatin-dependent neurotoxicity without negatively influence chemotherapy
Influence of resting tension on protease-activated receptor-mediated relaxation in guinea-pig tracheas.
Relaxin up regulates inducile nitric oxide synthase expression and nitric oxide generation in rat coronary endothelial
Relaxin activates the L-arginine-nitric oxide pathway in vascular smooth muscle cells in culture.
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