Direct immuno- fluorescence for the diagnosis of legionellosis

DJM HALDANE, R PEPPARD, RK SUMARAH. Direct immunofluor escenc e for t he d iagnosis of legionellosis. Can J Infect Dis 1993;4(2):101-104. Culture and direct immunofluorescent m icroscopy (DFA) results for Legionellapneumophila were reviewed over a two-year period. In the first year, a positive result was defined as having at least one morphologically typical fluorescing organism. In the second year. a positive was defined as at least five typical fluorescing organisms. Despite these stricter criteria and other measures to reduce the possibility of reagent contamination, there was no statistically significant difference in the sensitivity or specificity of the DFA in the two years for sputa, deep specimens or overall. Of 37 sputum specimens from infected patients, 16 were positive on DFA. Thirty-two of38 positive patients were detected by sputum culture. DFA can provide rapid diagnostic information but cannot be used to rule out the diagnosis. Sputum is a useful specimen for the initial laboratory investigation of patients with legionellosis. de donner des renseignements diagnostiques rapides. mais elle ne peut etre utilisee pour eliminer les diagnostics. L'expectoration est un prelevement utile pour le premier examen de laboratoire des patients atteints de legionellose