In Vitro

Jaboticaba is a fruit from a native tree to Brazil, Plinia peruviana. Jaboticaba peels are an important source of antioxidant molecules such as phenolic compounds. This study aimed to evaluate in vitro the activity of a hydroalcoholic extract of jaboticaba fruit peels (HEJFP) in wound healing processes and antioxidant activity in murine fibroblasts (L929 cell line). HEJFP concentrations (0.5, 1, 5, 10, 25, 50, 100, and 200 µg/mL) were tested in MTT assay and cell proliferation was verified at 100 µg/mL after 24 h and at 25, 50, and 100 µg/mL after 48 h of extract exposure. Evaluation of antioxidant activity was performed at 0.5, 5, 25, 50, and 100 µg/mL HEJFP concentrations. Cell treatment with HEJFP at 25, 50, and 100 µg/mL for 24 h followed by H2O2 exposure for 3 h showed a strong cytoprotective effect. In vitro scratch wound healing assay indicated that none of tested HEJFP concentrations (0.5, 5, 25, 50, and 100 µg/mL) were capable of increasing migration rate after 12 h of incubation. These results demonstrate a positive effect of HEJFP on the wound healing process on L929 fibroblasts cell line, probably due to the antioxidant activity exhibited by phytochemicals in the extract

Fentanyl-induced reward seeking is sex and dose dependent and is prevented by D-cysteine ethylester

Introduction: Despite their inclination to induce tolerance, addictive states, and respiratory depression, synthetic opioids are among the most effective clinically administered drugs to treat severe acute/chronic pain and induce surgical anesthesia. Current medical interventions for opioid-induced respiratory depression (OIRD), wooden chest syndrome, and opioid use disorder (OUD) show limited efficacy and are marked by low success in the face of highly potent synthetic opioids such as fentanyl. D-Cysteine ethylester (D-CYSee) prevents OIRD and post-treatment withdrawal in male/female rats and mice with minimal effect on analgesic status. However, the potential aversive or rewarding effects of D-CYSee have yet to be fully characterized and its efficacy could be compromised by interactions with opioid-reward pathology.Methods: Using a model of fentanyl-induced conditioned place preference (CPP), this study evaluated 1) the dose and sex dependent effects of fentanyl to induce rewarding states, and 2) the extent to which D-CYSee alters affective state and the acquisition of fentanyl-induced seeking behaviors.Results: Fentanyl reward-related effects were found to be dose and sex dependent. Male rats exhibited a range-bound dose response centered at 5 µg/kg. Female rats exhibited a CPP only at 50 µg/kg. This dose was effective in 25% of females with the remaining 75% showing no significant CPP at any dose. Pretreatment with 100 mg/kg, but not 10 mg/kg, D-CYSee prevented acquisition of fentanyl seeking in males while both doses were effective at preventing acquisition in females.Discussion: These findings suggest that D-CYSee is an effective co-treatment with prescribed opioids to reduce the development of OUD

Áreas de produção de algodão em Mato Grosso: nematoides, murcha de fusarium, sistemas de cultivo, fertilidade e física de solo.

A produção de algodão no estado de Mato Grosso é comprovadamente eficiente e rentável. A cada safra aumentam a tecnificação da cultura e o conhecimento acumulado. Reflexo disso é o crescimento da área ocupada por essa cultura em Mato Grosso, que representa mais de 50% do algodão plantado no país. No entanto, problemas relacionados à ocorrência de nematoides e doenças a eles associadas vêm crescendo e preocupando os produtores. Existem vários exemplos de inviabilidade de áreas em função desses parasitas, principalmente em locais que foram tradicionais no cultivo do algodoeiro. Práticas de manejo deverão ser incentivadas para reduzir ou pelo menos manter estável o problema. Para que a melhor estratégia possa ser utilizada, além de quantificar quais são as espécies presentes, é fundamental conhecer o sistema de produção no qual atua o produtor, o histórico da área, bem como as condições físicas e químicas do solo. O trabalho aqui relatado enfoca um esforço nesta direção, realizado em parceria entre o Instituto Mato-grossense do Algodão (IMAmt), a Associação Mato-grossense dos Produtores de Algodão (AMPA), o Instituto Brasileiro do Algodão (IBA), a Associação dos Produtores de Sementes de Mato Grosso (Aprosmat) e quatro unidades da Empresa Brasileira de Pesquisa Agropecuária (Embrapa): a Embrapa Agrossilvipastoril, Embrapa Algodão, Embrapa Instrumentação e Embrapa Agropecuária Oeste.bitstream/item/115841/1/cpamt-2014-matos-producao-algodao-mt.pd