The Relationship between Tumor Necrosis Factors-alpha (TNF-alpha)and Oxidative Stress in Iraqi Patients with Asthma

Asthma is the most common chronic disease of the airways, involving the imbalance of oxidative and antioxidative agents. There is an increased free radical generation and a decreased antioxidant enzyme activity. This study designed to find out the relation between TNF –alpha and oxidative stress in Iraqi patients with asthma. Seventy subject were enrolled in this study ,twenty patients of them with Asthma and twenty five subjects who are apparently healthy were taken as a control group. Serum TNF-alpha was determined by using enzyme- linked immunosorbent assay (ELISA).Serum levels of TNF-alpha  was highly  significantly increase in  asthma group compared with control group (p<0.01) and a significant positive correlation between TNF and malondialdehyde ( MDA) by affecting TNF  level in patients with Asthma  . Keywords : Asthma , TNF- ? , oxidative stres

Cardiovascular Diseases Correlation with Trace Elements in Hilla City

Cardiovascular disease (CVD) is a leading cause of global morbidity and mortality covers any disease of the circulatory system, however “trace elements” refers to chemical elements present in a natural material at concentrations  < 0.1 %wt, besides the minor elements (0.1 - 1 %wt) and the major elements (> 1%wt).This study include 60 patients  of  acute myocardial infraction ( AMI) and (40) healthy people . The results Showed that copper has increased in patients compared to control group , while  zinc ,magnesium, and selenium decreased in patients compared to control group. Keywords Cardiovascular diseases, Trace elements, Atomic absorption spectrophotometer, Toxicity

Antibacterial Activity of Citrullus Colocynthis against different types of bacteria

In the present study, antibacterial efficiency of citrullus colocynthesis (L.) schrad was Studied on seven types of pathogenic bacteria isolated from different sites infection, Escherichia. coli, Klebsiella pneumonia, Proteus mirabilis, Streptococcus pneumonia ,Streptococcus agalactia, Streptococcus mutans and Staphylococcus aureus. Water and ethanol extract were used for antibacterial assay. The inhibitory effects of this extracts were compared with standard antibiotics, cefotaxime. The ethanolic extract showed inhibitory activity against Escherichia. Coli, Proteus mirabilis, Staphylococcus aureus, Streptococcus agalactia. Water extract exhibited less or no activity against all types of bacteria. Results Indicated that ethanolic extract has a similar inhibitory effect with cefotaxime. Keywords: citrullus colocynthesis, ethanol extracts, cefotaxime, antibacterial activit

Inhibitory effect of new ligand 2, 3, 5, 6, 0, 0, 0, 0- tetra acetic acid-L- ascorbic acid on pathogenic bacteria

The inhibitory effect of new product (2, 3, 5, 6, 0, 0, 0, 0- tetra acetic acid-L- ascorbic acid)(V-C-4Ac) on growth of different types of pathogenic bacteria was investigated. Different concentrations were prepared from (V-C-4Ac) , (1%, 3%,  4% and 5%) nearly completely inhibited the growth of Streptococcus pneumoniae and Streptococcus agalactiae after 24 hrs incubation, and the same range of concentrations did not inhibit the growth of Streptococcus mutans, Klebsiella pneumonia and Proteus mirabilis. Also, this study shows  low effect of new ligand in the growth of Staphylococcus aureus and E. coli in high concentration (5%) after 2 hrs incubation. Keywords: L- ascorbic acid, New ligand synthesis (V-C,4Ac) , Antibacterial effect

Antibacterial Activity of 2- (2-Hydroxy phenylimino) Acetic Acid

The ability of 2-(2-hydroxy phenylimono) acetic acid [2-(2- HP-AA)]  to inhibit growth of different types of pathogenic bacteria was investigated.Different concentration were prepared from this chemical compounds( 40 mM ,50 mM and 70 mM). E.coli, staphylococcus aureus and streptococcus agalactiae nearly completely inhibited at 70 mM ,while the same concentration did not inhibit the growth of  Proteus mirabilis ,streptococcus mutans, Klebsiella pneumoniae and streptococcus pneumonia .after 24 h incubating in the present study we evaluate that 70 mM considered as the minimum inhibitory concentration. Key words: Acetic acid, Acetic acid derivatives 2-(2- HP-AA) , Antibacterial effect , Cytoplasmic P

Role of Leukotriene in Gastric Ulcer Induced by Acetyl Salicylic Acid in Male Rabbits : Gastroprotection by Montelukast

Gastric mucosal damage is a common disorder of the gastrointestinal system. Non- steroidal anti-inflammatory drugs (NSAID) are known to be aggressive agents for gastric ulcer development. Leukotrienes  play an important role in gastric mucosal damage induced by NSAIDs. Montelukast , a selective reversible Cysteinyl leukotriene receptor 1 antagonist, was reported to have beneficial effects in management of experimental gastric mucosal ulceration. This study designed to evaluate  the role of leukotriene against acetyl salicylic acid-induced gastric mucosal damage and to evaluate the gastroprotective activity of montelukast . Thirty local domestic male rabbits had been used in this study , divided into five groups as follows: normal control group, acetylsalicylic acid ( ASA) treated group, Omeprazole pretreatment group, montelukast pretreatment group, montelukast alone  treatment group. At the end of the experiment, the stomach of rabbit  is removed to prepare the tissue homogenate .  The results revealed that after administration of ASA significantly increase the mean ulcer index along with the LTD4 concentration, LTB4 concentration . But in presence of  montelukast  there is a significant decreased in the mean ulcer index along with  LTD4 concentration, LTB4 concentration .  These results suggest that the leukotrienes play an important role in  acetyl salicylic acid induced gastric ulcerations and gastroprotective activity of montelukast can be attributed to decreased activity of leukotrienes in gastric mucosa . Keywords :  Gastric ulcer , H.pylori , Non-steroidal anti-inflammatory drugs , montelukas

Biological Application of New Organic Derivatives on Bacterial Growth

The growth of strains of deferent types of pathogenic bacteria isolated from different sites of infection , Escherichia coli ,klebisiella pneumonia ,staphylococcus aurous ,staphylococcus epidermis , streptococcus pneumonia, streptococcus agalactiae , streptococcus mutans, has been investigated about inhibitory effect of new synthesized complex organic compound consisting of ( Ni+2 or Co+2) . The results reveled that cobalt (II) complex which contain chelating dicarboxylate ligands has action inhibitory effect on such pathogenic bacteria while Ni ( II) Shows no effect even in highly concentration but only on the streptococcus agalactiae. Key word : Pathogenic bacteria , Diacetic acid  derivative , antibacterial activity .

The Relationship of Immunoglobulin-E and Oxidative Stress in Iraqi patients with asthma

Asthma is a disease characterized by airway chronic inflammation and bronchial hyperactivity, involving the imbalance of oxidative and antioxidative agents. There is an increased free radical generation and a decreased antioxidant enzyme activity. Fifty subject were enrolled in this study .twenty patients with Asthma . Thirty subjects who are apparently healthy were taken as a control group. Serum IgE was determined by using enzyme- linked immunosorbent assay (ELISA) .MDA was determined by using colorimetric method .Serum levels of IgE was highly  significantly increase in  asthma group compared with control group (p<0.01).A significant positive correlation between IgE and MDA. MDA affect IgE level in patients with Asthma . Key words : Immunoglobulin – E , Oxidative stress, Asthm

Novel Natural Anti Gout Medication Extract from Momdica charantia

Medicinal plants were evaluated for their Xanthine Oxidase (XO) inhibitory potential. Their aqueous extracts, prepared from used parts, were tested in vitro, at 100 ?g/mL concentration, for their inhibition potencies expressed as % inhibition of XO activity. Two of the test plants were found inhibition% activity of xanthine oxidase. Namely Zingiber officinat (81.56±3.76) , Momdica charantia (96.5±2.17).Total Phenolic of Momdica charantia (80.83±0.30) was more than Zingiber officinat (62.18±0.65).Fraction of Momdica charantia (coumarin) was achieved the highest activity (inhibition activity of xanthine oxidase 97%) based on analysis HPLC. Screening of xanthine oxidase activity by coumarin in terms of kinetic parameter revealed uncompetitive mode of inhibition where Km 25Mm-1,Vmax 0.38 Mm min-1.Data showed that coumarin (0.5 mg/kg) treatment cause significant reduction in the serum uric acid level of hyperuricemia in normal mice. These finding suggest that Momdica charantia extract possess prominent medicinal properties and can be exploited as natural drug to treat diseases associated with free radical formation , oxidative stress and xanthine oxidase activity. Keywords : Gout , Allpurinol , Xanthine Oxidase , Momdica charanti

Sperm Mediated Gene Transfer in Mammals; a Versatile Platform with Multiple Enhancements Techniques

In the field of animal transgenesis, many attempts have been made recently to simplify facilitate, and reduce the cost and labor required to do such tasks. Although several transgenesis techniques (such as DNA microinjection and somatic cell nuclear transfer) have been applied successfully to produce transgenic animals, these traditional techniques are so tedious and have several disadvantages. Retroviral mediated gene transfer has solved some of these usual problems but has, however, inevitable disadvantages represented most prominently by its biological hazard. Many researchers found that the most simple and non-cost effective way to produce transgenic animals is to focus on the natural ability of the sperm to “carry” the foreign DNA and to “fertilize” the oocyte. The most important breakthrough obtained in this aspect is the accumulated information that demonstrated the ability of foreign DNA to be internalized into the sperm head after simple incubation step. Accordingly, the only manipulation step is restricted into the head of the sperm. Then, nature will be allowed to fulfill its scheduled task of reproduction. This method known as sperm mediated gene transfer or SMGT. However, simple incubation of naked DNA with sperm head is not efficient enough to integrate the foreign DNA into the genome of the sperm. Thus, this review aims to pave the way for every effort to enable the researchers to undergo the transgenesis experiments in the routine laboratories. This is potentially can be done by testing the validity of the most modern enhancement approaches suggested on the original SMGT