Antioxidant Activity and Anticarcinogenic Properties of Sisik Naga(Drymoglossum Piloselloides Presl.)

The research was conducted to determine the anticarcinogenic properties of sisik naga(Drymoglossum piloselloides Presl.), by the microculture tetrazolium salt (MTT) assay on the human breast carcinoma dependenthormone (MCF-7) cell lines. The preliminary results showed that the sisik naga extract displayed the cytotoxic effects against MCF-7 with IC50 value of 83.63g/ml. The antioxidative activity of the extracts which could contribute to their cytotoxic properties was also studied. The sisik naga extract was found to have high antioxidant activity with IC50-value of 4.229 ppm. The strong cytotoxic properties of the sisik naga extract could be due to its high antioxidant activity

Chemical Constituents And Possible Anticancer Properties Of Lawsonia Inermis And Strobilanthes Crispus

Studies on the cytotoxicity effects, antitumour components and their mechanism of action of S. crispus and L. inermis were carried out in this study. The nutrients and non-nutrients composition of L. inermis were determined to investigate whether the leaves are suitable for herbal tea preparation. The crude protein, ether extract, ash and moisture contents were also determined. Ash samples were further analysed to investigate water insoluble ash, water-soluble ash and alkalinity according to the International standard ISO 1576- 1975 (E) and ISO 1 578- 1975 (E) methods, whereas acid insoluble ash was also determined. Carbohydrate content was determined following the Anthrone method. In fresh L. inermis leaves, the moisture content forms the bulk of tissue weight with a mean value of 69.4%. This plant was found to contain high amounts of minerals, such as potassium (29455 mg/100g), calcium (2654 mg/1 00g), sodium (4094 mg/ 100g), iron (266 mg/100g) and phosphorus (550 mg/ 100g)

Antioxidant Activity and Anticarcinogenic Properties of “Sisik Naga”(Drymoglossum piloselloides Presl.)

The research was conducted to determine the anticarcinogenic properties of “sisik naga”(Drymoglossum piloselloides Presl.), by the microculture tetrazolium salt (MTT) assay on the human breast carcinoma dependenthormone (MCF-7) cell lines. The preliminary results showed that the “sisik naga” extract displayed the cytotoxic effects against MCF-7 with IC50 value of 83.63µg/ml. The antioxidative activity of the extracts which could contribute to their cytotoxic properties was also studied. The “sisik naga” extract was found to have high antioxidant activity with IC50-value of 4.229 ppm. The strong cytotoxic properties of the “sisik naga” extract could be due to its high antioxidant activity

Pengaruh Pemberian Ekstrak Daun Kola (Cola Nitida) Pada Kualitas Sperma Manusia in Vitro

Studi terhadap tanaman yang berpengaruh terhadap proses reproduksi masih menjadi skala prioritas. Salah satunya adalah Kola (Cola nitida), yang dikenal mempunyai efek stimulan. Telah dilakukan studi untuk mengetahui pengaruh ekstrak daun Kola terhadap viabilitas, motilitas dan integritas membran spermatozoa manusia in vitro. Sampel sperma diperoleh dari 20 orang pria dengan kategori normozoospermia. Sampel semen dibagi menjadi 1 kelompok kontrol ( kontrol negatif) dan 2 kelompok perlakuan dengan ekstrak daun Kola (0.05% dan 0.025%). Hasil penelitian menunjukkan bahwa ekstrak daun Kola tidak berpengaruh terhadap viabilitas spermatozoa tetapi secara bermakna meningkatkan motilitas dan integritas membran spermatozoa (p<0.05). Hasil ini mengindikasikan bahwa ekstrak daun Kola berpengaruh terhadap kualitas spermatozoa manusia in vitro.Study on medicinal plants that have potential effect on reproductive process is still important to be done. One of the plant is Kola (Cola nitida) that has a stimulant effect. This study evaluated the effects of Kola leaf extract toward viability, motility and membrane integrity on human sperm in vitro. Normozoospermia semens from twenty volunteers were taken and divided into 3 groups: 1 group was treated as control and 2 groups were treated with Kola leaf extracts (0.05% dan 0.025%). The results showed that Kola leaf extract had no significant effect in viability but increased significantly (p<0.05) the motility and membrane integrity. It indicates that Kola leaf extract influences human semen quality in vitro

Pengaruh Pemberian Ekstrak Daun Kola (Cola nitida) Pada Kualitas Sperma Manusia In Vitro

Studi terhadap tanaman yang berpengaruh terhadap proses reproduksi masih menjadi skala prioritas. Salah satunya adalah Kola (Cola  nitida), yang dikenal mempunyai efek stimulan. Telah dilakukan studi untuk mengetahui pengaruh ekstrak daun Kola terhadap viabilitas, motilitas dan integritas membran spermatozoa manusia in vitro. Sampel sperma diperoleh dari 20 orang pria dengan kategori normozoospermia. Sampel semen dibagi menjadi 1 kelompok kontrol ( kontrol negatif) dan 2 kelompok perlakuan dengan ekstrak daun Kola (0.05% dan 0.025%). Hasil penelitian menunjukkan bahwa ekstrak daun Kola tidak berpengaruh terhadap viabilitas spermatozoa tetapi secara bermakna meningkatkan motilitas dan integritas membran spermatozoa (p0.05). Hasil ini mengindikasikan bahwa ekstrak daun Kola berpengaruh terhadap kualitas spermatozoa manusia in vitro.Study on medicinal plants that have potential effect on  reproductive process is still important to be done. One of the plant is Kola (Cola nitida) that has a stimulant effect. This study evaluated  the effects of Kola  leaf  extract  toward viability, motility and membrane integrity on human sperm in vitro. Normozoospermia semens from  twenty volunteers were taken and divided into 3 groups: 1 group  was  treated  as control  and 2 groups were treated with Kola leaf extracts  (0.05% dan 0.025%). The results showed that Kola leaf extract  had no significant effect in viability but increased significantly (p0.05) the motility and membrane integrity. It indicates that Kola leaf extract influences human semen quality in vitro

Effect of flow resistance in open rectangular channel

The determination of flow resistance for open channels remains a challenge in practices. In this research, an experimental study was carried out to investigate the hydraulic roughness characteristics in an open channel. The experimental flume (10 m length, 0.30 m width and 0.46 height) was carried out with two conditions ; contain gravel bed and without gravel bed (as controller). Blocks of concrete foam were fabricated and laid on the bed surface for providing uniformly roughened along the open channel. A velocity flow meter was used to quantify the average velocity, and other parameters such as cross-section, hydraulic radius, wetted perimeter, and channel slopes were also calculated. Finally, the flow resistance, n obtained using the flume with gravel bed surface is higher than the flume without gravel bed surface. The flow conditions were declared as subcritical as the Froude number is less than 1 for both conditions in the flume. As a conclusion, it was identified that the hydraulic roughness n, was influenced by the type bed roughness, flow rate and channel slope

Potential anticancer effect of red spinach (Amaranthus gangeticus) extract.

The objective of this study was to determine the anti cancer effects of red spinach (Amaranthus gangeticus Linn) in vitro and in vivo. For in vitro study, microtitration cytotoxic assay was done using 3-(4,5-dimethylthiazol-2-il)-2,5- diphenil tetrazolium bromide (MTT) kit assay. Results showed that aqueous extract of A gangeticus inhibited the proliferation of liver cancer cell line (HepG2) and breast cancer cell line (MCF-7). The IC50values were 93.8 μg/ml and 98.8 μg/ml for HepG2 and MCF-7, respectively. The inhibitory effect was also observed in colon cancer cell line (Caco-2), but a lower percentage compared to HepG2 and MCF-7. For normal cell line (Chang Liver), there was no inhibitory effect. In the in vivo study, hepatocarcinogenesis was monitored in rats according to Solt and Farber (1976) without partial hepatectomy. Assay of tumour marker enzymes such as glutathione S-transferase (GST), gamma-glutamyl transpeptidase (GGT), uridyl diphosphoglucuronyl transferase (UDPGT) and alkaline phosphatase (ALP) were carried out to determine the severity of hepatocarcinogenesis. The result found that supplementation of 5%, 7.5% and 10% of A. gangeticus aqueous extract to normal rats did not show any significant difference towards normal control (P <0.05). The exposure of the rats to chemical carcinogens diethylnitrosamine (DEN) and 2-acetylaminofluorene (AAF) showed a significant increase in specific enzyme activity of GGT, GST, UDPGT and ALP compared to normal control (P <0.05). However, it was found that the supplementation of A. gangeticus aqueous extract in 5%, 7.5% and 10% to cancer-induced rats could inhibit the activity of all tumour marker enzymes especially at 10% (P 0.05). Supplementation of anti cancer drug glycyrrhizin at suggested dose (0.005%) did not show any suppressive effect towards cancer control (P <0.05). In conclusion, A. gangeticus showed anticancer potential in in vitro and in vivo studies

Determination of total antioxidant activity in three types of local vegetables shoots and the cytotoxic effect of their ethanolic extracts against different cancer cell lines.

Antioxidants play an important role in inhibiting and scavenging radicals, thus providing protection to humans against infections and degenerative diseases. Literature shows that the antioxidant activity is high on herbal and vegetable plants. Realizing the fact, this research was carried out to determine total antioxidant activity and the potential anticancer properties in three types of selected local vegetable shoots such as Diplazium esculentum (paku shoot), Manihot utillissima (tapioca shoot) and Sauropous androgynus (cekur manis). The research was also done to determine the effect of boiling, on total antioxidant activity whereby samples of fresh shoots are compared with samples of boiled shoots. In every case, antioxidant activity is compared to alpha-tocopherol and two methods of extraction used are the organic and the aqueous methods. Besides that, two research methods used were the ferric thiocyanate (FTC) and thiobarbituric acid (TBA) with absorbance of 500nm and 532nm respectively. Oneway ANOVA test at P D. esculentum (fresh) > S.androgynus (fresh) > M.utilissima (boiled) > D. esculentum (boiled) > S.androgynus (boiled). It also was found that S.androgynus shoots ethanolic extract was able to inhibit the viability of the breast cancer cell lines, MDA-MB-231 with the IC50 value of 53.33 μg/ml. However, S.androgynus shoots and D. esculentum shoots ethanolic extracts did not inhibit the viability of MDA-MB-231 cell line. While, the tapioca shoot ethanolic extract was able to inhibit the viability of MCF-7 cell line with the IC50 value of 52.49 μg/ml. S.androgynus shoots and D.esculentum shoots ethanolic extracts did not give an IC50 value against the MCF-7 cell line. S.androgynus, tapioca and D.esculentum shoots ethanolic extracts did not show cytotoxic effect against the Caco-2 and HepG2. There was no IC50-value from any sample against Chang Liver cell line. In conclusion, the antioxidant activity of both fresh and boiled samples were higher than alpha-tocopherol, although fresh vegetable shoots were found to be higher in antioxidant activity compared to boiled shoots. This study also suggested that S.androgynus shoots and tapioca shoots have potential as an anticancer agent against certain breast tumours

Peningkatan Pengetahuan Faktor-Faktor yang Memengaruhi Perilaku Seks dalam Upaya Cegah Seks Pranikah pada Siswa-Siswi SMP N 77 dan SMA N 77 Jakarta Pusat

ABSTRACT The premarital sex behavior is a problem and social phenomenon that is  increasingly prevalent in society. At a young age, the premarital sex should be a major concern to reduce pregnancy rates unintended, sexually transmitted diseases, school drop out, abortion and even a death. One effort to prevent the premarital sex behavior for students in schools with methods of providing knowledge about sexual behavior, reproductive health, an understanding of religious and the early marriage law. Evaluation of  indicators usingpre test and post test data that are  analyzed with paired t and regression test. The results show that after counseling, the knowledge of junior high school students about sexual behavior, reproductive health and religious knowledge increased significantly (p <0.05), while high school students of religious knowledge and early marriage law increased significantly.  Keywords: premarital sex; reproductive health; sexual behavio

Effects of strobilanthes crispus extract on the apoptotic pathway of human liver carcinoma cell lines

Previous study has been shown the potential anticarcinogenic effect of Strobilanthes crispus, a plant native to countries from Madagascar to Indonesia, on human liver cancer (HepG-2) cell lines. The objective of the present study was to investigate the mechanism of anticarcinogenic effectof S.crispus extract through apoptotic pathway. Exposure of HepG-2 cells to S.crispus extract resulted in induction of apoptosis in a dose-dependent manner as measured by Fluoresence Microscopy and Confocal Laser Scanning Microscopy analysis. These findings provide important new insight into the possible molecular mechanisms of the anticancer activity of S.crispus