Comparison of Fluorescein Isothiocyanate-Conjugated and Texas-Red-Conjugated Nucleotides for Direct Labeling in Comparative Genomic Hybridization

Fil: Larramendy, Marcelo Luis. Cátedra de Citología. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Elrifai, Wa`el. Department of Medical Genetics. Haartman Institute. University of Helsinki. Helsinki; FinlandFil: Knuutila, Sakari. Laboratory of Medical Genetics. Helsinki University. Central Hospital. Helsinki; Finlan

Optimization of comparative genomic hybridization using fluorochrome conjugated to Dctp and Dutp nucleotides

Fil: Elrifai, Wa`el. Department of Medical Genetics. Haartman Institute. University of Helsinki. Helsinki; FinlandFil: Larramendy, Marcelo Luis. Cátedra de Citología. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Björkqvist, Anna-Maria. University of Helsinki; FinlandFil: Hemmer, Samuli. Department of Oncology. Helsinki University Central Hospital. Helsinki; FinlandFil: Knuutila, Sakari. Laboratory of Medical Genetics. Helsinki University. Central Hospital. Helsinki; Finlan

Comparative Genomic Hybridization Reveals Differences in DNA Copy Number Changes Between Sporadic Gastric Carcinomas and Gastric Carcinomas from Patients with Hereditary Nonpolyposis Colorectal-Cancer

Comparative genomic hybridization was used to search for DNA copy number changes in samples of gastric cancer from 12 hereditary nonpolyposis colon cancer (HNPCC) patients and in samples of sporadic gastric carcinoma from 13 patients. The gastric cancer samples from HNPCC patients showed gains affecting 19q, Xp, and whole chromosome 22, each in a single patient. Neither high-level amplifications nor losses of DNA copy number were detected. On the other hand, 10 of the 13 (77%) sporadic gastric carcinoma samples had multiple DNA copy number changes. The most frequent gains occurred with minimal common overlapping regions at 1q22–q31, 8q23–qter, 17p11.2–q22, and 20q, all at a frequency of 31%. High-level amplifications were also seen at 17q21 in three cases (23%). Losses were rare, and the most frequent loss was with a minimal common overlapping region at 4q32 (23%). This suggests that multiple DNA copy number changes are needed for the development of sporadic gastric carcinoma but not for gastric carcinoma in HNPCC patients